Keratin-tannic acid complex nanoparticles as pH/GSH dual responsive drug carriers for doxorubicin

Du, Jinsong; Wang, Lijuan; Han, Xiao; Dou, Jun; Yuan, Jiang; Shen, Jian

doi:10.1080/09205063.2021.1906074

Cited by 11 publications

(7 citation statements)

References 36 publications

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“…The MTT assay, conducted on murine fibroblast cells, demonstrated that the toxicity of the free drug was higher than the cytotoxicity of DKNPs. Furthermore, the in vitro cytotoxicity test on lung carcinoma cells confirmed the anti- Recently, in 2021, Du et al [85] investigated the preparation of keratin-tannic acid complex nanoparticles, to produce a pH/GSH dual-responsive drug carrier for doxorubicin. In particular, tannic acid (TA) is usually employed as a non-toxic crosslinker for proteins [86,87], and in this work it formed complex nanoparticles together with keratin via a self-assembly process with non-covalent interactions, including hydrogen bonding and hydrophobic interaction [88].…”

Section: Self-assembly And/or Aggregationmentioning

confidence: 86%

“…Recently, in 2021, Du et al [85] investigated the preparation of keratin-tannic acid complex nanoparticles, to produce a pH/GSH dual-responsive drug carrier for doxorubicin. In particular, tannic acid (TA) is usually employed as a non-toxic crosslinker for proteins [86,87], and in this work it formed complex nanoparticles together with keratin via a self-assembly process with non-covalent interactions, including hydrogen bonding and hydrophobic interaction [88].…”

Section: Self-assembly And/or Aggregationmentioning

confidence: 99%

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Bioactive Keratin and Fibroin Nanoparticles: An Overview of Their Preparation Strategies

et al. 2022

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In recent years, several studies have focused their attention on the preparation of biocompatible and biodegradable nanocarriers of potential interest in the biomedical field, ranging from drug delivery systems to imaging and diagnosis. In this regard, natural biomolecules—such as proteins—represent an attractive alternative to synthetic polymers or inorganic materials, thanks to their numerous advantages, such as biocompatibility, biodegradability, and low immunogenicity. Among the most interesting proteins, keratin extracted from wool and feathers, as well as fibroin extracted from Bombyx mori cocoons, possess all of the abovementioned features required for biomedical applications. In the present review, we therefore aim to give an overview of the most important and efficient methodologies for obtaining drug-loaded keratin and fibroin nanoparticles, and of their potential for biomedical applications.

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Section: Self-assembly And/or Aggregationmentioning

confidence: 86%

Section: Self-assembly And/or Aggregationmentioning

confidence: 99%

Bioactive Keratin and Fibroin Nanoparticles: An Overview of Their Preparation Strategies

et al. 2022

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“…DOX-Cu-MOFs@Keratin was incubated in simulated TME consisting of 10 mM GSH and 1 mg/mL trypsin at low pH (5). Drug release curve were measured as previously described (Du et al, 2021a).…”

Section: Drug Releasementioning

confidence: 99%

“…Human hair keratin is an ideal shell for MOFs due to its biodegradability and stability under normal OPEN ACCESS EDITED BY physiological conditions. Compared with PEG and zwitterionic polymer, human hair keratin can effectively avoid the immune response and allergic reactions (Du et al, 2021a). On the other hand, it can easily disintegrate in the tumor microenvironment (TME) in response to low pH, and high levels of glutathione (GSH) and trypsin, which is conducive to release of the drug cargo in the tumor tissues (Du et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

Multi-stimuli responsive Cu-MOFs@Keratin drug delivery system for chemodynamic therapy

Chen

Yuan

et al. 2023

Front. Bioeng. Biotechnol.

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Although the potential of metal-organic framework (MOF) nanoparticles as drug delivery systems (DDS) for cancer treatment has been established by numerous studies, their clinical applications are still limited due to relatively poor biocompatibility. We fabricated a multifunctional Cu-MOFs@Keratin DDS for loaded drug and chemodynamic therapy (CDT) against tumor cells. The Cu-MOFs core was prepared using a hydrothermal method, and then loaded with the anticancer drug DOX and wrapped in human hair keratin. The Cu-MOFs@Keratin was well characterized by transmission electron microscopy (TEM), fourier transform infrared spectroscopy (FTIR), dynamic light scattering (DLS), and X-ray photoelectron spectroscopy (XPS). Characterization and pharmacokinetic studies of Cu-MOFs@Keratin were performed in vitro and in vivo. The keratin shell reduced the cytotoxicity and potential leakage of Cu-MOFs to normal cells, and allowed the drug-loaded nanoparticles to accumulate in the tumor tissues through enhanced permeability and retention effect (EPR). The particles entered the tumor cells via endocytosis and disintegrated under the stimulation of intracellular environment, thereby releasing DOX in a controlled manner. In addition, the Cu-MOFs produced hydroxyl radicals (·OH) by consuming presence of high intracellular levels of glutathione (GSH) and H2O2, which decreased the viability of the tumor cells.

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“…Very recently, Shen and colleagues described the production of nanoparticles made of a keratin-tannic acid complex loaded with DOX [52]. Tannic acid is a natural polyphenol generally used as a non-toxic cross-linker for proteins.…”

Section: Doxorubicin-loaded Human Hair Keratin-based Nanoparticlesmentioning

confidence: 99%

Keratin-Based Nanoparticles as Drug Delivery Carriers

Ferroni

Varchi

2021

Applied Sciences

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Keratin is a structural protein of mammalian tissues and birds, representing the principal constituent of hair, nails, skin, wool, hooves, horns, beaks, and feathers, and playing an essential role in protecting the body from external harassment. Due to its intrinsic features such as biocompatibility, biodegradability, responsiveness to specific biological environment, and physical–chemical properties, keratin has been extensively explored in the production of nanocarriers of active principles for different biomedical applications. In the present review paper, we aimed to give a literature overview of keratin-based nanoparticles produced starting from human hair, wool, and chicken feathers. Along with the chemical and structural description of keratin nanoparticles, selected in vitro and in vivo biological data are also discussed to provide a more comprehensive framework of possible fields of application of this protein. Despite the considerable number of papers describing the production and use of keratin nanoparticles as carries of anticancer and antimicrobial drugs or as hemostatic and wound healing materials, still, efforts are needed to implement keratin nanoparticles towards their clinical application.

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Keratin-tannic acid complex nanoparticles as pH/GSH dual responsive drug carriers for doxorubicin

Cited by 11 publications

References 36 publications

Bioactive Keratin and Fibroin Nanoparticles: An Overview of Their Preparation Strategies

Bioactive Keratin and Fibroin Nanoparticles: An Overview of Their Preparation Strategies

Multi-stimuli responsive Cu-MOFs@Keratin drug delivery system for chemodynamic therapy

Keratin-Based Nanoparticles as Drug Delivery Carriers

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