Keratin film made of human hair as a nail plate model for studying drug permeation

In vitro evaluation of the antifungal efficacy of poloxamer 407-based formulations in an infected nail plate model

“…1. In brief, bovine hoof plates (thickness: 100 µm) and keratin films (KF) (thickness: 120 µm, manufactured according to Lusiana et al, (2011)) were established as artificial nail plate models and infected with the dermatophyte fungus T. rubrum (strain DSM 19959, obtained from German Collection of Microorganisms and Cell Cultures, DSMZ, Braunschweig, Germany) on a potato glucose agar. After 7 days of incubation, the infected membranes were transferred onto a Sabouraud dextrose agar and a polyamide ring was glued with silicone paste (Baysilone-Paste, high viscous from GE Bayer Silicones, delivered by Carl Roth GmbH, Karlsruhe, Germany) onto the membrane.…”

Transungual permeation: current insights

“…An easy, stable, reproducible model with minimum variation is desirable for studying transungual delivery. To fulfill this requirement, Lusiana et al [85] developed keratin films derived from human hair for studying transungual permeation and antifungal efficacy. The permeability of film was studied by examining permeation of three markers, sodium fluorescein, rhodamine B, and fluorescein isothiocyanate, and was compared with bovine hooves, respectively.…”

Analysis of keratin films as screening tools for predicting the efficacy of potential hair dyes

“…Keratin can be extracted from a variety of sources such as human hair [7][8][9][10][11][12][13][14][15][16][17][18][19][20][21][22][23][24][25][26] and nails, 12 chicken feathers, 27 and wool, [28][29][30][31][32][33][34] and fabricated into thin films. Keratin films have been principally studied for their use as biomaterials, [7][8][9][10]12,15,[20][21][22][23][24][25][27][28][29][30][31][32][33][34][35][36][37]…”