Keratin-dopamine conjugate nanoparticles as pH/GSH dual responsive drug carriers

Han, Xiao; Wang, Lijuan; Du, Jinsong; Dou, Jun; Yuan, Jiang; Shen, Jian

doi:10.1080/09205063.2020.1803182

Cited by 9 publications

(6 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The new C–S peak was not noted in reactions A1 and A6, which showed only slight polymerization. For all samples, the peak intensity at 3290 cm –1 increased dramatically, likely due to an addition of a hydroxyl group in the newly synthesized catechol …”

Section: Resultsmentioning

confidence: 99%

“…One group developed PDA-coated keratin films for improved photoactivity and keratin–PDA hydrogels for enhanced antioxidant properties. , A recent study focused on improving the mechanical properties of keratin hydrogels using oxidized dopamine as a cross-linker for keratin . Another research created keratin–dopamine nanoparticles by amide bond formation as drug carriers . To the best of our knowledge, no studies on esthetic properties and skin applications have been performed on the combination of keratin and melanin.…”

Section: Introductionmentioning

confidence: 99%

“…4 Another research created keratin−dopamine nanoparticles by amide bond formation as drug carriers. 31 To the best of our knowledge, no studies on esthetic properties and skin applications have been performed on the combination of keratin and melanin. This article describes the formation of novel keratin− melanin (KerMel) molecules that form submicron particles with tunable colors that comply with skin tones.…”

Section: ■ Introductionmentioning

confidence: 99%

See 2 more Smart Citations

In Situ Synthesis of Keratin and Melanin Chromophoric Submicron Particles

Nowogrodski,

Damatov,

Sapru

et al. 2023

ACS Omega

View full text Add to dashboard Cite

In humans, melanin plays an esthetic role, dictating hair and skin color and traits, while keratin is the protein that comprises most of the epidermis layer. Eumelanin and pheomelanin are types of melanin synthesized from the same building blocks via enzymatic oxidation. Pheomelanin has an additional building block, cysteine amino acid, which affects its final structure. Keratin contains high cysteine content, and by exploiting free thiols in hydrolyzed keratin, we demonstrate the formation of keratin–melanin (KerMel) chromophoric submicron particles. Cryo-TEM analyses found KerMel particle sizes to be 100–300 nm and arranged in the form of a central keratin particle with polymerized l-dopa chains. Attenuated total reflection (ATR)-FTIR, UV–vis, and fluorescence measurements identified new chemical bonds, indicating the formation of KerMel particles. Finally, KerMel replicated natural skin tones and proved cytocompatibility for human epidermal keratinocytes at concentrations below 0.1 mg/mL. Taken together, KerMel is a novel, tunable material that has the potential to integrate into the cosmetic industry.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: ■ Introductionmentioning

confidence: 99%

See 1 more Smart Citation

In Situ Synthesis of Keratin and Melanin Chromophoric Submicron Particles

Nowogrodski,

Damatov,

Sapru

et al. 2023

ACS Omega

View full text Add to dashboard Cite

show abstract

“…On the same topic, Shen and co-workers reported the preparation of keratin-PDA conjugates for the production of biodegradable nanoparticles carrying DOX [48] by exploiting the ability of PDA to polymerize under mild conditions. For their work, the authors extracted keratin from hair in the form S-carboxymethyl keratin that was subsequently reacted with dopamine under EDC•HCl/NHS standard conditions.…”

Section: Doxorubicin-loaded Human Hair Keratin-based Nanoparticlesmentioning

confidence: 99%

Keratin-Based Nanoparticles as Drug Delivery Carriers

Ferroni

Varchi

2021

Applied Sciences

View full text Add to dashboard Cite

Keratin is a structural protein of mammalian tissues and birds, representing the principal constituent of hair, nails, skin, wool, hooves, horns, beaks, and feathers, and playing an essential role in protecting the body from external harassment. Due to its intrinsic features such as biocompatibility, biodegradability, responsiveness to specific biological environment, and physical–chemical properties, keratin has been extensively explored in the production of nanocarriers of active principles for different biomedical applications. In the present review paper, we aimed to give a literature overview of keratin-based nanoparticles produced starting from human hair, wool, and chicken feathers. Along with the chemical and structural description of keratin nanoparticles, selected in vitro and in vivo biological data are also discussed to provide a more comprehensive framework of possible fields of application of this protein. Despite the considerable number of papers describing the production and use of keratin nanoparticles as carries of anticancer and antimicrobial drugs or as hemostatic and wound healing materials, still, efforts are needed to implement keratin nanoparticles towards their clinical application.

show abstract

“…Wang et al fabricated a DDS consisting of sulfhydryl-linked keratin, which was highly stable in a simulated blood environment . Furthermore, Han et al coated drug-loaded nanoparticles with keratin for targeted release in the TME (Han et al, 2020). In a previous study, we used keratin to package drug-loaded mesoporous silica nanoparticles, and found that the keratin shell prevented drug leakage in a simulated physiological environment (Du et al, 2021b).…”

Section: Introductionmentioning

confidence: 99%

Multi-stimuli responsive Cu-MOFs@Keratin drug delivery system for chemodynamic therapy

Chen

Yuan

et al. 2023

Front. Bioeng. Biotechnol.

Self Cite

View full text Add to dashboard Cite

Although the potential of metal-organic framework (MOF) nanoparticles as drug delivery systems (DDS) for cancer treatment has been established by numerous studies, their clinical applications are still limited due to relatively poor biocompatibility. We fabricated a multifunctional Cu-MOFs@Keratin DDS for loaded drug and chemodynamic therapy (CDT) against tumor cells. The Cu-MOFs core was prepared using a hydrothermal method, and then loaded with the anticancer drug DOX and wrapped in human hair keratin. The Cu-MOFs@Keratin was well characterized by transmission electron microscopy (TEM), fourier transform infrared spectroscopy (FTIR), dynamic light scattering (DLS), and X-ray photoelectron spectroscopy (XPS). Characterization and pharmacokinetic studies of Cu-MOFs@Keratin were performed in vitro and in vivo. The keratin shell reduced the cytotoxicity and potential leakage of Cu-MOFs to normal cells, and allowed the drug-loaded nanoparticles to accumulate in the tumor tissues through enhanced permeability and retention effect (EPR). The particles entered the tumor cells via endocytosis and disintegrated under the stimulation of intracellular environment, thereby releasing DOX in a controlled manner. In addition, the Cu-MOFs produced hydroxyl radicals (·OH) by consuming presence of high intracellular levels of glutathione (GSH) and H2O2, which decreased the viability of the tumor cells.

show abstract

Keratin-dopamine conjugate nanoparticles as pH/GSH dual responsive drug carriers

Cited by 9 publications

References 29 publications

In Situ Synthesis of Keratin and Melanin Chromophoric Submicron Particles

In Situ Synthesis of Keratin and Melanin Chromophoric Submicron Particles

Keratin-Based Nanoparticles as Drug Delivery Carriers

Multi-stimuli responsive Cu-MOFs@Keratin drug delivery system for chemodynamic therapy

Contact Info

Product

Resources

About