KeraSkin™-VM: A novel reconstructed human epidermis model for skin irritation tests

Jung, Kyoung Mi; Lee, Su Hyon; Jang, Won Hee; Jung, Haeng Sun; Heo, Yong; Park, Young Ho; Bae, Seung Jin; Lim, Kyung Min; Seok, Seung Hyeok

doi:10.1016/j.tiv.2014.02.014

Cited by 64 publications

(44 citation statements)

References 12 publications

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“…Of note, the activities of 1000 μg/mL (-)-4OH-ST ( 1 ) were comparable to those of 20,000 μg/mL kojic acid, suggesting that (-)-4OH-ST ( 1 ) may be active in humans. The toxicity of (-)-4OH-ST was further investigated in a 3D reconstituted human epidermis model, Keraskin™ (Biosolution Co, Seoul, Korea) [21], which showed little effects on tissue viability (Figure 6c).…”

Section: Resultsmentioning

confidence: 99%

Anti-Pigmentary Effect of (-)-4-Hydroxysattabacin from the Marine-Derived Bacterium Bacillus sp.

et al. 2017

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Bioactivity-guided isolation of a crude extract from a culture broth of Bacillus sp. has led to the isolation of (-)-4-hydroxysattabacin (1). The inhibitory effect of (-)-4-hydroxysattabacin (1) was investigated on melanogenesis in the murine melanoma cell line, B16F10, and human melanoma cell line, MNT-1, as well as a pigmented 3D-human skin model. (-)-4-Hydroxysattabacin treatment decreased melanin contents in a dose-dependent manner in α-melanocyte stimulating hormone (α-MSH)-stimulated B16F10 cells. Quantitative real time PCR (qRT–PCR) demonstrated that treatment with (-)-4-hydroxysattabacin down-regulated several melanogenic genes, including tyrosinase, tyrosinase-related protein 1 (TRP-1), and tyrosinase-related protein 2 (TRP-2) while their enzymatic activities were unaffected. The anti-melanogenic effects of (-)-4-hydroxysattabacin were further demonstrated in a pigmented 3D human epidermal skin model, MelanodermTM, and manifested as whitening and regression of melanocyte activation in the tissue.

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Section: Resultsmentioning

confidence: 99%

Anti-Pigmentary Effect of (-)-4-Hydroxysattabacin from the Marine-Derived Bacterium Bacillus sp.

et al. 2017

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“…The testing methods base on these four models have been referred to define the alternative test methods endorsed by OECD to assess skin corrosion and skin irritation . In addition, other culture skin models, such as the Leiden reconstructed human epidermal model, the KeraSkin ™ model and epiCS ™ model, have been reported in the evaluation and validation efforts for in vitro skin tolerance testing according to ECVAM performance standard .…”

Section: Discussionmentioning

confidence: 99%

“…The testing methods base on these four models have been referred to define the alternative test methods endorsed by OECD to assess skin corrosion and skin irritation [11,12,14,16,22,[27][28][29][30]. In addition, other culture skin models, such as the Leiden reconstructed human epidermal model, the KeraSkin TM model and epiCS TM model, have been reported in the evaluation and validation efforts for in vitro skin tolerance testing according to ECVAM performance standard [10,31,32]. China EpiSkin model was reconstructed with keratinocytes of Chinese origin, and this is the first Chinese reconstructed human epidermis model to meet the high quality standard with industrialized production criteria.…”

Section: Discussionmentioning

confidence: 99%

Establishment and characterization of a reconstructed Chinese human epidermis model

Qiu¹,

Zhong²,

Zhou³

et al. 2015

Intern J of Cosmetic Sci

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“…Four RhE models, EpiSkin ™ , EpiDerm ™ SIT (EPI-200), the SkinEthic ™ RhE, and LabCyte EPI-MODEL24 SIT were accepted as OECD TG 439 (85) and Keraskin ™ was in the pre-validation stage (86). Generally RhE SIT is similar in procedure with some minor difference to optimize the predictive capacity (Table 2).…”

Section: Alternatives To Draize Rabbit Skin Irritation Testmentioning

confidence: 99%

“…After exposed for 45 min, the tissues were rinsed with D-PBS. Tissues were post-incubated for 42 hr and then evaluated viability with MTT assay (86). …”

Section: Alternatives To Draize Rabbit Skin Irritation Testmentioning

confidence: 99%

Alternatives toIn VivoDraize Rabbit Eye and Skin Irritation Tests with a Focus on 3D Reconstructed Human Cornea-Like Epithelium and Epidermis Models

2017

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Human eyes and skin are frequently exposed to chemicals accidentally or on purpose due to their external location. Therefore, chemicals are required to undergo the evaluation of the ocular and dermal irritancy for their safe handling and use before release into the market. Draize rabbit eye and skin irritation test developed in 1944, has been a gold standard test which was enlisted as OECD TG 404 and OECD TG 405 but it has been criticized with respect to animal welfare due to invasive and cruel procedure. To replace it, diverse alternatives have been developed: (i) For Draize eye irritation test, organotypic assay, in vitro cytotoxicity-based method, in chemico tests, in silico prediction model, and 3D reconstructed human cornea-like epithelium (RhCE); (ii) For Draize skin irritation test, in vitro cytotoxicity-based cell model, and 3D reconstructed human epidermis models (RhE). Of these, RhCE and RhE models are getting spotlight as a promising alternative with a wide applicability domain covering cosmetics and personal care products. In this review, we overviewed the current alternatives to Draize test with a focus on 3D human epithelium models to provide an insight into advancing and widening their utility.

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KeraSkin™-VM: A novel reconstructed human epidermis model for skin irritation tests

Cited by 64 publications

References 12 publications

Anti-Pigmentary Effect of (-)-4-Hydroxysattabacin from the Marine-Derived Bacterium Bacillus sp.

Anti-Pigmentary Effect of (-)-4-Hydroxysattabacin from the Marine-Derived Bacterium Bacillus sp.

Establishment and characterization of a reconstructed Chinese human epidermis model

Alternatives toIn VivoDraize Rabbit Eye and Skin Irritation Tests with a Focus on 3D Reconstructed Human Cornea-Like Epithelium and Epidermis Models

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