Establishment and characterization of a reconstructed Chinese human epidermis model

Qiu, Jie; Zhong, Lingyan; Zhou, Meng; Chen, D; Huang, Xin-Zhu; J, Chen; Chen, M; Ni, Haiyan; Cai, Zhenzi

doi:10.1111/ics.12249

Cited by 16 publications

(26 citation statements)

References 35 publications

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“…Reconstructed human epidermis such as EpiSkin has been proposed to study skin permeability, irritancy, corrosiveness and phototoxicity . Aside from its histological structure and cell morphology akin to native epidermis, RHE can also express markers of epidermal proliferation and differentiation . Therefore, we further investigate the effect of Ad‐HA‐FoxO1 infection on keratinocyte differentiation and involucrin expression in EpiSkin.…”

Section: Resultsmentioning

confidence: 99%

FoxO1 enhances differentiation and apoptosis in human primary keratinocytes

Shi

Liao

Cai

et al. 2018

Experimental Dermatology

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Forkhead box-O1 (FoxO1) is a key nutrient- and growth factor-dependent regulator of metabolism, but its functional role in human primary keratinocytes (HPKs) is less known. To investigate the role of FoxO1 in HPKs and effect of insulin-like growth factor 1 (IGF-1) and isotretinoin on FoxO1 expression, HPKs were treated with 1.2 mmol/L calcium chloride, 1-20 ng/mL IGF-1 and 0.1-10 μmol/L isotretinoin. Recombinant adenovirus expressing FoxO1 or FKHR shRNA lentivirus transfection was introduced to upregulate or silence FoxO1 expression. Epidermal FoxO1 immunostaining was lower in acne lesion than in normal skin. FoxO1 overexpression induced involucrin expression, G2/M arrest and apoptosis but suppressed proliferation, while FoxO1 silencing decreased involucrin expression but increased proliferation, S phase and viable cells in HPKs. IGF-1 downregulated FoxO1 and involucrin but upregulated p-Akt expression in HPKs, which was blocked by pretreatment with LY294002. Isotretinoin enhanced FoxO1, p53 and p21 but inhibited p-FoxO1 and involucrin expression in HPKs. These results demonstrate that FoxO1 promotes differentiation and apoptosis in HPKs. IGF-1 may reduce keratinocyte differentiation through PI3K/Akt/FoxO1 pathway, while isotretinoin can reinforce FoxO1 expression. FoxO1 may be involved in acne pathogenesis and could serve as a potential therapeutic target.

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Section: Resultsmentioning

confidence: 99%

FoxO1 enhances differentiation and apoptosis in human primary keratinocytes

Shi

Liao

Cai

et al. 2018

Experimental Dermatology

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“…The skin is the largest organ in the human body, representing up to 16% of body weight 22 . It is the boundary between an organism and the environment, acting as a defense organ protecting against the penetration of pathogens and external toxins, controlling the damage caused by the UV rays and preventing desiccation 23 . The skin consists of three main compartments: the epidermis, the dermis and the hypodermis 24 .…”

Section: Skin Structure and Functionsmentioning

confidence: 99%

Approaches to the development of 3d bioprinted skin models: the case of natura cosmetics

Millás

Lago

Vasquez-Pinto

et al. 2019

IJAMB

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We are close to achieving the production of a biomimetic functional skin and this advance is mainly due to the demand that is not limited to the field of regenerative medicine, the need for transplantation of this organ due to the aging of the population, but for ethical reasons related to the tests of safety and efficacy of new formulas in animal models by the cosmetic and pharmaceutical industries. The limitations involved in traditional 2D cell culture approaches and manual techniques for biomimetic generation have driven the use of innovative technologies such as 3D bioprinting. One of the main advantages of the bioprinted skin is the authenticity, scalability and reproducibility of tissues compared to conventional constructs, via precise positioning of multiple cell types and the inclusion of appendages. The models of bioprinted skins will serve as a platform for the development of new formulations, molecule testing, disease simulation, as well as an alternative to chronic wound biocuratives and clinical transplants. This paper reviews the state-of-the-art approaches available for skin model bioprinting, discusses the context of the drug-cosmetic industry in the adoption of these models and presents the characteristics of the project under development at Natura Cosmetics.

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“…Наиболее успешно в настоящее время разрабатываются реконструированные эквиваленты кожи человека. Например, такие коммерчески доступные модели как EpiSkinÔ, EpiDermÔ, SkinEthicÔ RHE и epiCS® широко используются для оценки специфической в отношении здоровья человека токсичности химических веществ и их смесей, фармацевтических препаратов, а также некоторых видов косметических продуктов [9]. Тесты in vitro на таких моделях одобрены Организацией экономического сотрудничества и развития (ОЭСР), включая исследования коррозии и раздражения кожи, абсорбции веществ через кожу и другие.…”

Section: Introductionunclassified

Quality control study of engineered skin tissue

Nakhod

Русанов²,

Druzhilovskiy

et al. 2018

BIOMED KHIM

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OMERO service was used to annotate the cell line HaCaT microscope images by two independent expert groups. The images were obtained in the course of developing tissue-engineered epithelium which consisted of several layers of the keratinocytes. Evaluation of expert opinions was performed by calculation of specificity, sensitivity and accuracy. The best convergence of opinions (91%) was achieved for the confluence of the cell monolayers. Accuracy 70% was observed in determining the extent of cell differentiation after 10 days of incubation. The paper illustrates the usefulness of OMERO service for dynamic cross-validation of quality in the development and standardization of cell preparations.

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Establishment and characterization of a reconstructed Chinese human epidermis model

Abstract: This is the first reconstructed Chinese human epidermis model reported to meet the high quality standard with industrialized production criteria. This China EpiSkin model can be used for both skin research and safety assessment in vitro.

Cited by 16 publications

References 35 publications

FoxO1 enhances differentiation and apoptosis in human primary keratinocytes

FoxO1 enhances differentiation and apoptosis in human primary keratinocytes

Approaches to the development of 3d bioprinted skin models: the case of natura cosmetics

Quality control study of engineered skin tissue

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