Keragaman Jenis Khamir Penghasil Etanol Yang Diisolasi Dari Makanan Fermentasi Di Kepulauan Riau

Sumerta, I Nyoman; Kanti, Atit

doi:10.47349/jbi/13012017/61

Cited by 6 publications

(5 citation statements)

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“…Goldhawke et al [54] reported that PCR amplification in the DNA ribosomal region D1/D2 using NL1/NL4 primers would produce DNA bands of 500 -700 bp in size. Sumerta and Kanti [55] reported that yeast isolates amplified using the same primer produced DNA bands of 600 bp in size. The nucleotide base sequences of a yeast isolate UNJCC Y-83, UNJCC Y-123 and UNJCC Y-84 were compared with those stored in the National Center for Biotechnology Information (NCBI) genebank.…”

Section: Identification Yeast Based On Morphological and Molecular Ch...mentioning

confidence: 99%

“…Meanwhile, a difference of 6 or more nucleotides (1 %) indicates that the species differ [53]. The Neighbor-joining method was used to reconstruct the phylogenetic tree with a bootstrap value of 1,000 replications in an effort to determine the lowest evolutionary gap between each yeast species [56,55]. The findings revealed that the yeast UNJCC Y-123 was in a monophyletic clade with yeast P. manshurica IFO 10726 type strain with 100 % bootstrap value, while the yeast UNJCC Y-83 and UNCC Y-84 were in a monophyletic clade with yeast S. cerevisiae NRLL Y 12632 type strain with a bootstrap value of 92 % (Figure 3) [57,58].…”

Section: Identification Yeast Based On Morphological and Molecular Ch...mentioning

confidence: 99%

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The Potential of Cellulolytic Yeast Pichia manshurica UNJCC Y-123, Saccharomyces cerevisiae UNCC Y-84, and Saccharomyces cerevisiae UNJCC Y-83 to Produce Cellulase Enzyme by Using Substrate Skin Delignification of Cocoa (Theobroma cocoa)

Sukmawati,

Balqis,

Adisyahputra

et al. 2023

Trends Sci

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Exploring the possibility of using agricultural waste as a substrate for the synthesis of cellulase enzymes for fuel and renewable energy is the main goal of this study, which is in line with the notions of sustainable development and environmental stewardship. The product of delignification of cocoa peel can be used as a substrate for cellulase enzymes producing by yeast isolated from Balinese palm wine. Cellulase enzymes made from yeast from Balinese palm wine can be produced using the delignified cocoa peel as a substrate. This study aims to analyze the cellulase enzyme activity of yeast from Bali's palm wine on delignified cocoa bark substrates. The tests carried out were delignification of cocoa bark (solvent variations: HCl, H2SO4 and NaOH; concentrations: 1, 1.5 and 2 %; biomass 1:15 and 1:20 (w/v)); screening of cellulolytic yeast from Balinese palm wine; molecular identification and morphological characteristics of yeast; and cellulase enzyme activity (variation of yeast isolates and fermentation time of 48, 72 and 96 h). The results of delignification of cocoa shells showed that the use of 1.5 % NaOH solvent with cocoa shell powder biomass of 1:15 (9.59 ± 0.11) significantly differed from the value of reducing sugar content. Based on the screening results of 6 yeast isolates, 3 yeast isolates with the highest cellulolytic index values were selected, namely UNJCC Y-83 (0.29 ± 0.01 mm), UNJCC Y-123 (0.24 ± 0.01 mm) and UNJCC Y-84 (0.23 ± 0.01 mm). For testing cellulase enzyme activity. S. cerevisiae UNJCC Y-83 (4.11 ± 0.41 U/mL); S. cerevisiae UNJCC Y-84 (4.11 ± 0.33 U/mL) and P. manshurica UNJCC Y-123 (4.06 ± 0.12 U/mL) at 96 h of fermentation had significantly different cellulase enzyme activity. The results of identification of yeast rDNA in the D1/D2 region with NL1/NL4 primers obtained the identity of Saccharomyces cerevisiae UNJCC Y-83; Saccharomyces cerevisiae UNJCC Y-84 (99.66 % homology); and Pichia manshurica (100 % homology). Theobroma cocoa rind can be used as a yeast substrate to produce cellulase enzymes for fuel and renewable energy. HIGHLIGHTS Screening of 6 yeast isolates, 3 yeast isolates with the highest cellulolytic index values were selected, namely UNJCC Y-83 (0.29 ± 0.01 mm), UNJCC Y-123 (0.24 ± 0.01 mm) and UNJCC Y-84 (0.23 ± 0.01 mm) for testing molecular identification and cellulase enzyme activity The result of rDNA sequence analysis in the D1/D2 region showed that the yeast isolates UNJCC Y-83 and UNJCC Y-84 had a homology level of 99.66 % as Saccharomyces cerevisiae with a bootstrap value of 92 %. UNJCC Y-123 yeast isolate has a homology level of 100 % as Pichia manshurica with a bootstrap value of 100 % cerevisiae UNJCC Y-83 (4.11 ± 0.41 U/mL); S. cerevisiae UNJCC Y-84 (4.11 ± 0.33 U/mL); and P. manshurica UNJCC Y-123 (4.06 ± 0.12 U/mL) at 96 h of fermentation had significantly different cellulase enzyme activity Theobroma cocoa rind can be used as a yeast substrate to produce cellulase enzymes for fuel and renewable energy GRAPHICAL ABSTRACT

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Section: Identification Yeast Based On Morphological and Molecular Ch...mentioning

confidence: 99%

Section: Identification Yeast Based On Morphological and Molecular Ch...mentioning

confidence: 99%

The Potential of Cellulolytic Yeast Pichia manshurica UNJCC Y-123, Saccharomyces cerevisiae UNCC Y-84, and Saccharomyces cerevisiae UNJCC Y-83 to Produce Cellulase Enzyme by Using Substrate Skin Delignification of Cocoa (Theobroma cocoa)

Sukmawati,

Balqis,

Adisyahputra

et al. 2023

Trends Sci

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“…Pohon filogenetik direkonstruksi menggunakan Neighbour joining dan diuji dengan menggunakan Bootstrap method (Sumerta et al, 2017).…”

Section: Pohon Filogenetikunclassified

Identifikasi Molekular Bakteri Glukanolitik Indigenous KE-B6 dari Saluran Pencernaan Bekicot (Achatina fulica)

2019

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Glucanolitic bacteria are bacteria that have the ability to break down glucan into glucose monomer units. The ability of the bacteria is caused by the presence of glucanase enzymes. The choice of glucanase derived from bacteria is based on the ability and speed of bacterial growth in terms of producing glucanase enzymes. The presence of bacteria and protozoa in the digestive tract symbiotic with each other to digest cellulose or concomitant materials . Based on the ability of the way of life to digest forage and leaf litter, it is suspected that snails (Achatina fulica) have the ability to produce glucanase biocatalysts, especially in the digestive tract. To find out the characteristics and characteristics of indigenous bacteria snail canals, identification of KE-B6 bacteria is carried out molecularly so that accurate and accurate results are obtained. The Basic Alignment Search Tools BLAST results of KE-B6 bacterial isolates based on 16S rDNA sequence data with 27F (Forward) and 1492R (Reverse) primers showed that these bacterial isolates had homology of 99.64% to Serratia marcescens. Key words: bacteria, glucanolitic, A. fulica, Serratia marcescens.

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“…Yeast has been long utilized in various fermented food and beverage productions, such as beer, tapai, and wine. Yeast in the fermentation process acts to degrade substrates to formulate the structure, aroma, and texture in food and beverages (Sumerta & Kanti, 2017). Along with Jurnal Riset Biologi dan Aplikasinya https://journal.unesa.ac.id/index.php/risetbiologi technological advances, yeast utilization expands from the industrial sector to the energy sector that develops renewable energy such as bioethanol and biofuel as alternatives to fossil fuel.…”

Section: Introductionmentioning

confidence: 99%

Optimization of Various ITS rDNA Amplification Protocol of Yeast Isolated from Giant Honey Beehives (Apis dorsata)

S.Si

Firdhausi

Tyastirin

et al. 2021

JRBA

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Indonesia is a country with high variability of microorganisms, including bacteria, yeast, and fungi. Yeast isolates could be isolated from the honeycomb of Apis dorsata. Molecular approaches were used to identify yeast using ribosomal DNA gene sequences, called the ITS gene. The optimum condition for DNA extractions and amplifications are needed for the successfully of molecular identification. Therefore, it is necessary to optimize the DNA extraction and amplification of several protocols to obtain good identification results. This study aimed to compare the effects of DNA extraction with various temperatures and different amplification protocols. LIPI reference DNA extraction protocol with the boiling method and variations in incubation time of 10, 15, and 20 minutes at a temperature of 98° C. Meanwhile, for the amplification of yeast DNA using a variety of different amplification protocols. The results showed the optimal time of incubation was 10 minutes in K1 isolates with DNA purity of 1.896. meanwhile, for isolates K2, K3, and K4 each with a purity of 2.246, 2.335, and 1.748. optimal DNA amplification results were indicated by the presense of DNA bands for each sample K1, K2, K3, and K4, namely 503, 542, 492, and 526 bp. In this study, it can be concluded that the optimal incubation time for the extraction process is 10 minutes. In addition, the optimal amplification protocol was shown in the DNA bands in all sample.

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Keragaman Jenis Khamir Penghasil Etanol Yang Diisolasi Dari Makanan Fermentasi Di Kepulauan Riau

Cited by 6 publications

References 0 publications

The Potential of Cellulolytic Yeast Pichia manshurica UNJCC Y-123, Saccharomyces cerevisiae UNCC Y-84, and Saccharomyces cerevisiae UNJCC Y-83 to Produce Cellulase Enzyme by Using Substrate Skin Delignification of Cocoa (Theobroma cocoa)

The Potential of Cellulolytic Yeast Pichia manshurica UNJCC Y-123, Saccharomyces cerevisiae UNCC Y-84, and Saccharomyces cerevisiae UNJCC Y-83 to Produce Cellulase Enzyme by Using Substrate Skin Delignification of Cocoa (Theobroma cocoa)

Identifikasi Molekular Bakteri Glukanolitik Indigenous KE-B6 dari Saluran Pencernaan Bekicot (Achatina fulica)

Optimization of Various ITS rDNA Amplification Protocol of Yeast Isolated from Giant Honey Beehives (Apis dorsata)

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