Keragaman Genetik Gen Hormon Pertumbuhan (GH|MboII) pada Itik Sikumbang Janti Menggunakan Penciri PCR-RFLP

Nova, Tertia Delia; Yurnalis, Yurnalis; Sari, Anita Mayang

doi:10.25077/jpi.18.1.44-52.2016

Cited by 8 publications

(12 citation statements)

References 3 publications

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“…This study's primary length of the GH gene amplified was 879 base pairs (bp). The outcomes of this observe are not same from those of [23], who stated that the amplified primary length of the GH gene was 801 bp. This is due to the use of different primers.…”

Section: Discussioncontrasting

confidence: 59%

“…Mutation occurs around the cutting position. The outcomes of this observe are not a lot exclusive from the research of [23], who stated that diversity of GH genes in exon 1, which was cut with the MboII enzyme, showed that the GH gene of Sikumbang Janti ducks was polymorphic characterized by the appearance of genotypes (+/+), (+/-), and (-/-) and two alleles + and -. Sutopo et al [26] stated that the population of Demak and Pekalongan ducks found two alleles on the GH gene, (+) and (-), which formed homozygous characters (+/+) and (-/-) and heterozygous (+/) characters.…”

Section: Discussionmentioning

confidence: 77%

“…The results of DNA extraction showed that there were thick and thin bands indicating that the concentration of DNA produced was high and low. This is to the statement of several researchers who stated that high concentrations of DNA produced thick and bright bands, while a low concentration of DNA produced a thin and opaque band [22,23]. DNA extraction is said to be successful if there is no smear from the DNA bands [24].…”

Section: Discussionmentioning

confidence: 86%

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Morphometric characterization and effect of growth hormone (GH) gene polymorphism on growth traits of Kerinci duck (Anas platyrhynchos)

2022

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Objective: To determine the association of GH gene diversity with growth traits and to obtain the morphometric characteristics in Kerinci ducks.Methods: The research method is an experiment using 96 blood samples of Kerinci duck blood. The methods included data collection on quantitative characteristics (body weight, body weight gain, body measurements), blood samples from Kerinci ducks, DNA extraction activities, PCR amplification and restriction with AluI enzyme. Data analysis included t-test, T2-Hotelling, principal component analysis, genotype frequency, allele, Hardy-Weinberg balance, heterozygosity, and PIC.Results: The quantitative characteristics of male Kerinci ducks were significantly different (P<0.05) higher than female Kerinci ducks. Analysis of the Kerinci duck GH|AluI gene was polymorphic. The population of Kerinci ducks was in Hardy-Weinberg equilibrium (P>0.05). Diversity Value of Kerinci Duck Ho<He. Quantitative characteristics of GH gene of Kerinci duck genotype +/+ were significantly different (P<0.05) higher than genotype +/- and -/-.Conclusions: The quantitative characteristics of male Kerinci ducks were higher than female Kerinci ducks. Characteristics of the body size of Kerinci duck are the length of sternum, length of shank, and circumference of shank, and identifier of body shape is length of wings. The Kerinci duck GH|AluI gene is polymorphic and has associations with quantitative characteristics, with the best genotype being the +/+ genotype.

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Section: Discussioncontrasting

confidence: 59%

Section: Discussionmentioning

confidence: 77%

Section: Discussionmentioning

confidence: 86%

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Morphometric characterization and effect of growth hormone (GH) gene polymorphism on growth traits of Kerinci duck (Anas platyrhynchos)

2022

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“…DNA extraction aims to obtain DNA with excellent quality, which has high purity. DNA purification is a process to separate DNA from cell lysates (proteins, carbohydrates, lipids) and other contaminants (Hernandez et al, 2006;Nova et al, 2016). Bangkok chicken DNA extraction results from 50 blood samples were electrophoresed using 1.5% Agarose, which were visualized using UV light through Gel Doc presented in Figure 3.…”

Section: Dna Extractionmentioning

confidence: 99%

Phenotype Diversity and Gene Myostatin (MSTN) of Bangkok Chicken using PCR-RFLP

2021

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This study aims to characterize the phenotype and determine the diversity of the Myostatin (MSTN) gene at Bangkok chickens using the Polymerase Chain Reaction- Restriction Fragment Length Polymorphism (PCR-RFLP) method. This study used 50 Bangkok chicken blood samples taken from the axillary vein on the wing. DNA was extracted using the protocol Genomic DNA Purification Kit from Promega and then amplified by PCR (polymerase chain reaction) using a pair of primers F: 5'GGT TTT GAC GAC ATG AGC CT3' R: 5'CAG GTG GAA TGT CAT GCA GA3' with product length 955 bp. Amplification products were cut using restriction enzyme MboI with cutting site ↓GATC. MSTN|MboI fragments of the Bangkok chicken were electrophoresed using 2% agarose gel and visualized using doc gel. The average difference test (T-test) on body weight and weight gain of Bangkok chickens from DOC to 3 months by gender. Polymorphism analysis includes allele frequency and genotype. Male and female Bangkok chickens have low phenotype diversity. The MSTN|MboI gene fragment is monomorphic with band positions of 492 bp, 244 bp, and 219 bp resulting in a genotype of ++, and there is one type of allele with a + allele frequency of 100%.

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“…Salah satu gen kandidat yang berperan dalam peforma yang bersifat ekonomis yaitu Gen Growth hormone Receptor atau GHR (Yurnalis dan Sarbaini 2014;Nova et al 2016). GHR diduga mempengaruhi keragaman itik Sikumbang Janti.…”

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Keragaman Gen Growth Hormone Receptor (GHR) Ekson 10 pada itik Sikumbang Janti

Masti

Yurnalis

Nova

et al. 2021

JIPTHP

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Growth Hormone Receptor (GHR) gene play important roles in duck performances due to their crucialfunctions in growth and has been considered as a candidate gene for growth traits. This reasearch wasaimed to indification polymorphism of Growth Hormon Receptor (GHR) gene in Sikumbang Janti duckand this study used 68 blood duck. Polymerase chain reaction (PCR) and sequencing analysis wereconducted to detect SNP existence in exon 10 of GHR gene. The result of this study showed that wasfound two SNP at g.1112 A>G and g.1304 C>T with transision mutation in exon 10 GHR gene. Genotypesfound in the g.1112 A>G were AA and GG with types of Allele A and G. Genotypes found in the g.1304T>C the were TT and CC with types of Allele T and C. Polymorphism of GHR gene were polymorphicand Population of Sikumbang Janti duck in Hardy-Weinberg equilibrium. It could be concluded thatGHR gene has potential to can be marker genetic in Sikumbang Janti ducks.

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Keragaman Genetik Gen Hormon Pertumbuhan (GH|MboII) pada Itik Sikumbang Janti Menggunakan Penciri PCR-RFLP

Cited by 8 publications

References 3 publications

Morphometric characterization and effect of growth hormone (GH) gene polymorphism on growth traits of Kerinci duck (Anas platyrhynchos)

Morphometric characterization and effect of growth hormone (GH) gene polymorphism on growth traits of Kerinci duck (Anas platyrhynchos)

Phenotype Diversity and Gene Myostatin (MSTN) of Bangkok Chicken using PCR-RFLP

Keragaman Gen Growth Hormone Receptor (GHR) Ekson 10 pada itik Sikumbang Janti

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