KERAGAAN GENOTIPE DAN FENOTIPE IKAN UCENG Nemacheilus fasciatus (Valenciennes, 1846) ASAL BOGOR, TEMANGGUNG, DAN BLITAR

Ath-thar, Muhammad Hunaina Fariduddin; Ambarwati, Arifah; Soelistyowati, Dinar Tri; Kristanto, Anang Hari

doi:10.15578/jra.13.1.2018.1-10

Cited by 7 publications

(6 citation statements)

References 5 publications

(7 reference statements)

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“…Selain itu, sumber genetik yang digunakan terbatas sehingga potensi inbreeding bisa terjadi. Keragaman genetik yang menurun akibat pola migrasi yang lebih sempit terjadi pada ikan gurami dari berbagai strain pada heterozigositas dengan kisaran 0,0203-0,1735 (Nugroho et al, 2016), ikan tengadak memiliki heterozigositas dengan kisaran 0,09-0,18 , pada ikan mas rajadanu 0,12-0,20 (Cahyanti et al, 2014), dan pada ikan uceng memiliki kisaran nilai heterozigozitas 0,075-0,153 (Ath-thar et al, 2018). Berbeda dengan ikan hias rainbow Kurumoi hasil budidaya yang memiliki keragaman genetik yang tinggi dengan nilai heterozigositas yang tinggi, yaitu berkisar 0,2091-0,2479 (Hayuningtyas et al, 2016) dan pada ikan belida budidaya keragaman genetiknya juga tinggi yaitu 0,2922-0,2978 (Nugroho et al, 2019).…”

Section: Badan Tengah Midsectionunclassified

VARIASI GENETIK TIGA GENERASI IKAN HIAS CUPANG ALAM ENDEMIK DARI ACEH Betta rubra, Perugia 1893 (Pisces: Osphronemidae), HASIL BUDIDAYA

Hayuningtyas¹,

Kusrini²,

Sinansari³

et al. 2021

Jurnal Riset Akuakultur

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Betta rubra merupakan salah satu spesies ikan cupang alam endemik dari Aceh. Keberadaannya yang hampir dinyatakan punah sebelum ditemukan kembali pada tahun 2007. Tujuan dari penelitian ini adalah mengkaji keragaman genetik dan potensi genetik dari ikan Betta rubra dari tiga generasi yang sudah dibudidayakan untuk perbaikan genetik di Balai Riset Budidaya Ikan Hias (BRBIH), Depok, Jawa Barat, Indonesia. Jumlah sampel yang digunakan pada populasi G-0 adalah enam ekor, sedangkan pada populasi G-1 dan G-2 masing-masing 10 ekor. Ikan uji yang digunakan diambil sirip ekornya untuk analisis secara genotipe dengan randomly amplified polymorphic DNA (RAPD) menggunakan primer yaitu OPZ-9, OPB-6, dan OPZ-13. Sebelum diambil sirip ekornya ikan terlebih dahulu difoto di atas millimeter block untuk data truss morfometrik (fenotipe). Hasil menunjukkan ikan Betta rubra populasi alam (G-0) memiliki nilai heterozigositas 0,1872 dan derajat polimorfisme 47,06% yang lebih rendah dibandingkan generasi G-1 dengan heterozigositas 2,421 dan derajat polimorfisme 64,71%. Populasi G-2 memiliki nilai heterozigositas 0,1577 dan derajat polimorfisme 44,12%. Koefisien keragaman secara fenotipe populasi G-1 memiliki variasi lebih tinggi dibanding populasi G-0 dan G-2. Hubungan kekerabatan antara G-1 dengan G-0 dan G-2 berbeda nyata (P<0,05), sedangkan hubungan antara G-1 dengan G-2 tidak berbeda nyata (P>0,05), sehingga antara populasi G-0 dan G-2 membentuk cluster terpisah dengan G-1. Keragaman genetik pada tiga generasi Betta rubra memiliki pola yang sama baik secara fenotipe maupun genotipe.Betta rubra is one of the endemic species of Betta fish from Aceh. The fish was almost declared extinct before it was rediscovered in 2007. The purpose of this study was to examine the genetic diversity and genetic potential of Betta rubra from three generations which have been reared for genetic improvement at the Research Institute for Ornamental Fish Culture, Depok, West Java, Indonesia. The number of fish for G-0 population used in the study was six fish whilst G-1 and G-2 populations were 10 fish. Tail fins from each fish were sampled for genotype analysis using randomly amplified polymorphic DNA (RAPD) using primers OPZ-9, OPB-6, and OPZ-13. Before tail fin collection, the fish was photographed on a millimeter block for truss morphometric data measurement (phenotype). The results showed that the Betta rubra wild population (G-0) had heterozygosity of 0.1872 and polymorphism of 47.06% which were lower than the G-1 population with heterozygosity of 2.421 and polymorphism of 64.71%. The G-2 population had heterozygosity of 0.1577 and polymorphism of 44.12%. The phenotype coefficient of variation in the G-1 population higher than the G-0 and G-2 populations. The kinship relationship between G-1 with G-0 and G-2 was significantly different (P<0.05), while the relationship between G-1 and G-2 was not significantly different (P>0.05). This research concludes that the populations of G-0 and G-2 have formed a separate cluster to G-1. The genetic diversities in the three Betta rubra populations have similar phenotype and genotype patterns.

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Section: Badan Tengah Midsectionunclassified

VARIASI GENETIK TIGA GENERASI IKAN HIAS CUPANG ALAM ENDEMIK DARI ACEH Betta rubra, Perugia 1893 (Pisces: Osphronemidae), HASIL BUDIDAYA

Hayuningtyas¹,

Kusrini²,

Sinansari³

et al. 2021

Jurnal Riset Akuakultur

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“…Penelitian tentang ikan uceng terkait dengan proses domestikasi telah dilakukan (Tjahjo et al, 2017;Ath-thar et al, 2018). Penelitian tentang padat tebar pada ikan uceng yang diadaptasikan di lingkungan ex situ juga telah dilakukan dengan tingkat kepadatan yang masih rendah (Prakoso et al, 2016).…”

Section: Pendahuluanunclassified

PENGARUH PERBEDAAN PADAT TEBAR LARVA TERHADAP PERTUMBUHAN DAN SINTASAN PADA IKAN UCENG (Nemacheilus fasciatus)

et al. 2019

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Proper stocking density on larval rearing of barred loach (Nemacheilus fasciatus Valenciennes, 1846) is very important in terms of obtaining the best growth and survival rate. This study was aimed to observe the growth and survival rate of barred loach subjected to different larval stocking density. Fish larvae were obtained by artificial spawning from in aquarium. Larvae were stocked on aquariums (50 × 40 × 30 cm) with three stocking density treatments (5, 10, and 15 individuals/L) and three replications. The experimental fish larvae were fed by Artemia and commercial feed (crude protein content: 30%) and feeding frequency twice per day (ad libitum). The length and weight were reassured every 10 days during 70 days of rearing period. Temperature, pH, and dissolved oxygen were measured at each treatment. Data obtained from measurements were analysed to assess the length gain, weight gain, specific growth rate (SGR), biomass gain, and survival rate. The results showed length gain of 3.35 ± 0.01, 2.94 ± 0.10, and 2.82 ± 0.15 cm and weight gain of 0.573 ± 0.023, 0.436 ± 0.018, and 0.417 ± 0.043 g in larval stocking density of 5, 10 and 15 individuals/L, respectively. Specific growth rates and biomass growth were of 6.22 ± 0.41, 5.93 ± 0.21, and 5.68 ± 0.41 %/day and 94.04 ± 5.39, 146.80 ± 3.81, and 167.24 ± 17.48 g in larval stocking density of 5, 10 and 15 individuals/L, respectively. Meanwhile, the survival rates obtained in this study were 89.13 ± 7.25, 93.13 ± 5.13, and 76.50 ± 3.54 % in larval stocking density of 5, 10 and 15 individuals/L, respectively. Increased stocking density has a negative impact on the growth and survival rate of barred loach.

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“…Tarwinangsih et al (2011) states that the high level of genetic diversity can be indicated from the diversity of haplotype (Hd) which is the more diverse haplotypes it shows the higher of the genetic diversity. Ath-thar et al (2018) states genetic diversity affects the ability of a population to respond to environmental changes. The low haplotype diversity of this study concludes that Zebrafish has less adaptability to environmental changes.…”

Section: Haplotype Diversitymentioning

confidence: 99%

Phylogenetic Study of Zebrafish (Amatitlania nigrofasciata) as an Introduction Spesies in Buyan Lake

Pratama

Julyantoro

Pratiwi

2018

J. Mar. Aquat. Sci.

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Buyan Lake has some fishery commodities which were often encountered include the Zebrafish (Amatitlania nigrofasciata). The origin existence of Zebrafish in Buyan Lake is not yet known then it needs study in order to know the certainty of species, phylogenetic, haplotype diversity, and phenotype characteristic. The study was conducted from January to February 2018. Molecular identification was done at Laboratory of Biodiversity Indonesia Bali. This research used quantitative and qualitative descriptive methods. Sampling was done by simple random sampling from fisherman catches. Zebrafish’s fin samples were used for molecular identification and Zebrafish’s morphological for morphological identification. The results showed that the entire sequence samples were successfully amplified with the Cytochrome Oxidase subunit I (COI) gene of 620 bp. The genetic distance in the entire Zebrafish sequences in Buyan Lake of 0 which means that the sequence of all samples are exactly similiar as species of Amatitlania nigrofasciata with the genetic distance calculation of 0. Sequence of Zebrafish in Buyan Lake is closely related to Amatitlania nigrofasciata KU568740, Amatitlania nigrofasciata KU568739, Amatitlania nigrofasciata KU568737, Amatitlania nigrofasciata KJ552531, and Amatitlania nigrofasciata KU568738 with genetic distance of 0 and has a distant relation with Rocio ostofasciata EU751752 with genetic distance of 0.113. The haplotype diversity of Zebrafish sequences in Buyan Lake showed the value of 0 which belongs to a low haplotype diversity. Black and Black Zebrafish with Red Spotted are in a different clade with Albino and Albino Zebrafish with Red Spotted with bootstrap value of 100, means they have phenotypically differences.

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KERAGAAN GENOTIPE DAN FENOTIPE IKAN UCENG Nemacheilus fasciatus (Valenciennes, 1846) ASAL BOGOR, TEMANGGUNG, DAN BLITAR

Cited by 7 publications

References 5 publications

VARIASI GENETIK TIGA GENERASI IKAN HIAS CUPANG ALAM ENDEMIK DARI ACEH Betta rubra, Perugia 1893 (Pisces: Osphronemidae), HASIL BUDIDAYA

VARIASI GENETIK TIGA GENERASI IKAN HIAS CUPANG ALAM ENDEMIK DARI ACEH Betta rubra, Perugia 1893 (Pisces: Osphronemidae), HASIL BUDIDAYA

PENGARUH PERBEDAAN PADAT TEBAR LARVA TERHADAP PERTUMBUHAN DAN SINTASAN PADA IKAN UCENG (Nemacheilus fasciatus)

Phylogenetic Study of Zebrafish (Amatitlania nigrofasciata) as an Introduction Spesies in Buyan Lake

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