Kemampuan Konsorsium Bacillus pada Pupuk Hayati dalam Memfiksasi N2, Melarutkan Fosfat dan Mensintesis Fitohormon Indole 3-Acetic-Acid

Husna, Muhimatul; Sugiyanta, Sugiyanta; Pratiwi, Etty

doi:10.21082/jti.v43n2.2019.117-125

Cited by 5 publications

(6 citation statements)

References 14 publications

(15 reference statements)

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“…Spesies Bacillus yang dapat meningkatkan ketersediaan unsur hara nitrogen dan fosfat dalam tanah antara lain yaitu: B. subtilis, B. amiloliquefaciens dan B. pumilus. Menurut Mahdiah [19] Bacillus sp. menghasilkan asam organik seperti asam formiat, asam asetat, dan asam laktat yang berfungsi melarutkan fosfat dalam bentuk yang sulit larut.…”

Section: Identifikasi Dan Jumlah Mikroorganismeunclassified

Dekomposer Alami Berbahan Limbah Sayur Dengan Penambahan Whey Keju Sebagai Sumber Protein

Anita¹,

Faradila²,

Hendratama³

et al. 2022

ATMOSPHERE

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Timbunan limbah sayuran yang tidak terkendalikan dapat berdampak negatif yang akan memengaruhi berbagai segi kehidupan, baik secara langsung maupun tidak langsung. Banyak penelitian yang menggunakan limbah sayur sebagai bahan baku pembuatan pupuk dengan menambahkan EM4 sebagai sumber mikroorganisme pendegradasi. Penggunaan EM4 sebagai sumber mikroorganisme bisa digantikan dengan mikroorganisme lokal dari campuran limbah sayur kubis, whey keju, molase, bekatul dan air kelapa. Untuk itu dalam penelitian ini digunakan limbah sayuran yaitu kubis, dengan variasi waktu fermentasi dan rasio limbah kubis dan whey keju sebagai decomposer alami dari campuran limbah sayuran dan whey keju ini diproses secara fermentasi sehingga dapat digunakan sebagai sumber mikroorganisme dalam pembuatan pupuk organik cair (POC). Penelitian ini bertujuan untuk mempelajari bagaimana pengaruh waktu fermentasi dan pengaruh rasio dalam proses pembuatan decomposer alami dari limbah sayur kubis dan whey keju. Pada penelitian ini digunakan rasio 30:70; 20:80; 10:90; 0:100 pada campuran whey keju dan limbah sayur kubis (WLs) dengan waktu fermentasi selama 7, 14, 21 hari. Dari hasil analisa kadar Nitrogen diperoleh bahwa hasil tertinggi terdapat pada rasio WLs 20:80 sebesar 1,54 dengan waktu fermentasi 14 hari, sedangkan pada analisa kadar Fosfor diperoleh hasil tertinggi dengan rasio WLs 10:90 sebesar 0,77 pada waktu fermentasi 21 hari serta pada analisa kadar Kalium diperoleh hasil tertinggi dengan rasio WLs 30:70 sebesar 0,41 pada waktu fermentasi 21 hari. Sementara pada hasil analisa identifikasi dan jumlah mikroorganisme menunjukkan bahwa pembuatan larutan decomposer alami dengan waktu fermentasi 7 hari mengandung 1 jenis isolat bakteri asam laktat yaitu Bacillus subtilis serta tidak adanya bakteri patogen seperti E.coli dan salmonella dan didapatkan jumlah koloni mikroorganisme paling sedikit pada variabel WLs 20:80 yaitu sebesar 15 × 104 koloni/100 mL.

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Section: Identifikasi Dan Jumlah Mikroorganismeunclassified

Dekomposer Alami Berbahan Limbah Sayur Dengan Penambahan Whey Keju Sebagai Sumber Protein

Anita¹,

Faradila²,

Hendratama³

et al. 2022

ATMOSPHERE

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“…Bakteri pelarut fosfat dan pemfiksasi nitrogen diaplikasikan ke benih padi berupa pupuk hayati cair. Uji kemampuan bakteri untuk melarutkan fosfat dan memfiksasi nitrogen dalam pupuk hayati cair telah diteliti terlebih dahulu dan disajikan pada Husna et al (2019). Pupuk hayati yang digunakan terdiri dari bakteri Bacillus sp.…”

Section: Pendahuluanunclassified

“…Berdasarkan hasil penelitian sebelumnya oleh Husna et al (2019), bakteri Bacillus sp. yang diaplikasikan pada penelitian ini dapat memfiksasi nitrogen melalui uji nitrogenase, Bacillus sp.…”

Section: Pengaruh Aplikasi Bakteri Pelarut Fosfat Dan Fiksasi Nitrogen Terhadap Sifat Kimia Tanah Sawahunclassified

“…Berdasarkan hasil uji sekresi asam organik oleh bakteri yang digunakan pada penelitian ini, bakteri Bacillus sp. mensekresi empat jenis asam organik yaitu asam asetat, asam oksalat, asam laktat dan asam malat (Husna et al 2019). Asam oksalat lebih efektif melarutkan hara P daripada asam malat, selain itu asam organik juga mempengaruhi penyerapan hara P bagi tanaman (Panhwar et al 2013).…”

Section: Pengaruh Aplikasi Bakteri Pelarut Fosfat Dan Fiksasi Nitrogen Terhadap Sifat Kimia Tanah Sawahunclassified

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Respons Hasil Padi dan Hara Tanah Sawah terhadap Bakteri Pelarut Fosfat dan Pemfiksasi Nitrogen

Husna

Sugiyanta

Pratiwi³

2021

Agrotechnology Res J

Self Cite

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The condition of unfertile paddy field due to excessive inorganic fertilizer application causing soil degradation. Fertilizing techniques using phosphate solubilizing and nitrogen fixer bacteria are breakthroughs that should be developed in paddy cultivation to increase fertility soil and harvest of rice. This research aims to study on phosphate solubilizing and nitrogen fixer bacteria ability to increase the harvest of rice by increasing chemical and biological fertility of soil. The research was conducted in the greenhouse of Sawah Baru experimental garden, IPB University. The design used was a completely randomized design with one factor that was dosage of inorganic fertilizers (NPK) with seven levels that were control, NPK 100%, NPK 0% + bacteria, NPK 25% + bacteria, NPK 50% + bacteria, NPK 75% + bacteria and NPK 100% + bacteria. Each stage was repeated four times, so there were 28 units. The results of phosphate solubilizing and nitrogen fixer bacteria could produce more rice grain 0.32 ton.ha<sup>-1</sup> than NPK 100% (recommended dose). Biological soil fertility improved with the increased population of bacteria, but there was no improved soil chemical fertility of soil.

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“…OAs such as citric, malonic, oxalic, tartaric, and ketogluconic acids released by the roots can chelate Al and Fe ions to release P into the available form by replacing the position of the phosphate anion. Combined with cations Ca, Al, and Fe, they form complex compounds that are difcult to dissolve [26,27]. Some of the OAs produced by bacterial and fungal activity are citric, malic, fumaric, succinic, pyruvic, tartrate, oxaloacetic acids, 2-ketogluconate, lactosalt, propionate, and butyrate [28][29][30][31].…”

Section: Introductionmentioning

confidence: 99%

Improving Upland Acid Soil Properties And Increasing Maize Yield By Phosphate Rock Application With Organic Acids

Sutriadi

Anwar

Mulyanto

et al. 2022

International Journal of Agronomy

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Problems encountered in the management of upland acid soils, besides soil pH, are also low in phosphorus (P) content and less available to plants. The addition of organic acids (OAs) to the direct application of phosphate rock (PR) has not been widely studied yet and is expected to improve upland acid soil properties. The research aims to determine the effect of adding OAs on the direct application of PR to improve upland acid soil and increase maize yield. It was a greenhouse experiment using a randomized complete design in a factorial arrangement with three replications. The treatments consisted of four types of OA (citric, humic, succinic, and oxalic acid) and five OA concentration levels (0, 25, 50, 100, and 200 ppm). The hybrid maize (Zea mays L) was used as the test crop. The parameters observed were soil properties (pH, exchangeable H (H+), exchangeable Al (Al3+), exchangeable Ca (Ca2+), cation exchange capacity (CEC), available P (as P-Bray1)) and maize growth (plant heights and yield). The results show that the types of OA treatments had no significant effect on soil properties and maize yield; in contrast, the concentrations of OA levels were able to improve soil properties as indicated by a significant effect on pH, H+, Al3+, Ca2+, CEC, available P, and increased maize yield. An increase in soil pH due to the addition of OAs to the direct application of PR could increase Ca2+ and CEC and decrease Al3+ and H+, while increasing available P for plants. Although not significantly different, oxalic acid was able to improve the observed soil properties and maize yield, followed by citric, humic, and succinic acids. The maximum OA concentration was 126.9 ppm. We can say that the main finding of this research is that oxalic, citric, humic, and succinic acids at a concentration of 126 ppm can be applied to phosphate rock. In Indonesia, humic acid has been widely applied by farmers because it is easy to obtain. Therefore, it can be applied to phosphate rock.

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Kemampuan Konsorsium Bacillus pada Pupuk Hayati dalam Memfiksasi N2, Melarutkan Fosfat dan Mensintesis Fitohormon Indole 3-Acetic-Acid

Cited by 5 publications

References 14 publications

Dekomposer Alami Berbahan Limbah Sayur Dengan Penambahan Whey Keju Sebagai Sumber Protein

Dekomposer Alami Berbahan Limbah Sayur Dengan Penambahan Whey Keju Sebagai Sumber Protein

Respons Hasil Padi dan Hara Tanah Sawah terhadap Bakteri Pelarut Fosfat dan Pemfiksasi Nitrogen

Improving Upland Acid Soil Properties And Increasing Maize Yield By Phosphate Rock Application With Organic Acids

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