Keemun black tea extract contains potent fatty acid synthase inhibitors and reduces food intake and body weight of rats via oral administration

Abstract

The inhibitory effects of a black tea extract on fatty acid synthase were measured through inhibition kinetics. The Keemun black tea extract showed more potent inhibitory activity on fatty acid synthase than green tea extract. Additionally, the inhibitory ability of the black tea extract depended on the extracting solvent and the conditions used. Only 10-23% of the inhibitory activity from the black tea was extracted by the general method of boiling with water. The results suggested that the main fatty acid synthase inhibitors in black tea might be theaflavins. Which were more potent than epigallocatechin gallate or C75. The reaction site on the fatty acid synthase and the inhibition kinetics for the extract were different from those of epigallocatechin gallate or C75. In addition, Keemun black tea extract significantly reduced food intake, body weight and blood triglyceride of diet-induced obesity SD rats via oral administration.

Novel and potent inhibitors of fatty acid synthase derived from catechins and their inhibition on MCF-7 cells

“…The double-reciprocal plot ( Figure 2B) indicates that EG-2 is also a competitive inhibitor to NADPH; and the inhibition constant Ki was 0.40 mg/mL. Compared to EGCG, the Ki values of EG-2 are much lower, which indicates that EG-2 is a much stronger inhibitor of FAS [14].…”

Theaflavin Synthesized in a Selective, Domino-Type, One-Pot Enzymatic Biotransformation Method with <i>Camellia sinensis</i> Cell Culture Inhibits Weight Gain and Fat Accumulation to High-Fat Diet-Induced Obese Mice

“…14) TF (1) also inhibited both body weight gain and visceral fat accumulation, with no significant difference observed in the amount of food consumption, urination and faeces between the experimental and control mice. Further studies are warranted to elucidate the underlying mechanisms of TF (1).…”

“…1). These compounds are reported to elicit a variety of physiological effects, including anti-obesity, 1) glucose-lowering 2,3) and anticancer effects. 4) Therefore, TF (1) has become recognized as both a natural coloring agent and a bioactive substance.…”

Inhibitory Effects of Allium Vegetable Extracts on Fatty Acid Synthase

“…The weight-control and anti-cancer functions of teas and tea-polyphenols incarnate the double target character of FAS. The extracts of green tea and black tea, and their components gallated catechins and theaflavin had been reported to be effective inhibitors of FAS (Du et al, 2005;Wang and Tian, 2001;Zhang et al, 2006).…”

“…The weight-control and anti-cancer functions of teas and tea-polyphenols incarnate the double target character of FAS. The extracts of green tea and black tea, and their components gallated catechins and theaflavin had been reported to be effective inhibitors of FAS (Du et al, 2005;Wang and Tian, 2001;Zhang et al, 2006).Besides the well known garlic (Allium sativum L.) and onion (Allium cepa L.), several other Allium species are widely grown for culinary use, such as scallion (Allium fistulosum L.), chive (Allium schoenoprasum L.) and Chinese chive (Allium tuberosum L.). Since ancient times, these Allium vegetables have been used as common foods and for the treatment and prevention of many diseases, such as cancer, coronary heart disease, obesity, hyperlipidemia, diabetes, hypertension, cataract and disturbances of the gastrointestinal tract (Augusti, 1996).…”