Theaflavin Synthesized in a Selective, Domino-Type, One-Pot Enzymatic Biotransformation Method with &lt;i&gt;Camellia sinensis&lt;/i&gt; Cell Culture Inhibits Weight Gain and Fat Accumulation to High-Fat Diet-Induced Obese Mice

Takemoto, Masumi; Takemoto, Hiroaki; Saijô, Ryoyasu

doi:10.1248/bpb.b16-00284

Cited by 17 publications

(17 citation statements)

References 12 publications

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“…(7) In recent years, theaflavins of black tea have also attracted considerable interest, as they are shown to have various physiological actions, including antioxidant, (8) anticancer, (9) anti-atherosclerotic, (10) anti-inflammatory, (11) antiviral, (12) and anti-periodontitis (13) effects, as well as the prevention of osteoporosis (14) . Furthermore, these compounds have been shown to have human health benefits including glucose-lowering (15) and anti-obesity actions, (16) as a prevention of lifestyle-related diseases. In addition, theaflavin and thearubigin has also shown to possess antibacterial properties both in-vivo and in-vitro.…”

Section: B a C K G R O U N Dmentioning

confidence: 99%

Antibacterial Activity of Black Tea Extract against S. mutans, S. aureus, L. acidophilus, Klebsiella and E. coli

Goswami¹,

Kalita²,

Bhuyan³

2020

jemds

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BACKGROUND Drinking tea has antioxidant, anti-inflammatory and anti-carcinogenic properties. In addition, tea is also considered beneficial for cardiovascular health and oral health. Health benefits of green tea are attributed to its polyphenol content. Catechins are the major polyphenols in green tea and in black tea the catechins are oxidized to theaflavins. Polyphenols present in tea have exhibited antimicrobial effects against a wide range of pathogenic bacteria. Studies have shown that green tea catechins are bioavailable in both plasma and urine. The aim of the present study is to evaluate the antibacterial activity of black tea extract against standard strains of S. mutans, S. aureus, L. acidophilus, Klebsiella and E. coli. METHODS Black tea extract was prepared by boiling black tea leaves in distilled water. It was then filtered, and the filtrate was treated with chloroform and ethyl acetate. The ethyl acetate was evaporated in a rotary evaporator and a brown coloured residue was obtained. Its antibacterial activity was studied against standard strains of five common bacteria i.e.; S. mutans, L. acidophilus, S. aureus, Klebsiella spp, and E. coli and the MIC of the black tea extract was determined using serial dilution method. RESULTS Black tea extract showed sufficient antibacterial activity against the tested bacteria. The MIC of black tea extract was lowest against Staphylococcus aureus. CONCLUSIONS Tea extracts have significant antimicrobial activity at varying concentrations against different bacterial pathogens.

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Section: B a C K G R O U N Dmentioning

confidence: 99%

Antibacterial Activity of Black Tea Extract against S. mutans, S. aureus, L. acidophilus, Klebsiella and E. coli

Goswami¹,

Kalita²,

Bhuyan³

2020

jemds

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“…In recent years, TFs have attracted considerable interest, as they have been shown to have various physiological actions, including antioxidant [ 12 ], anticancer [ 13 ], anti-atherosclerotic [ 14 ], anti-inflammatory [ 15 ], antiviral [ 16 , 17 , 18 ], and anti-periodontitis [ 19 , 20 ] effects, as well as the prevention of osteoporosis [ 21 , 22 ]. Furthermore, these compounds have been shown to have human health benefits including glucose-lowering [ 23 , 24 , 25 , 26 ] and anti-obesity actions [ 10 , 27 , 28 ], as a prevention of lifestyle-related diseases.…”

Section: Introductionmentioning

confidence: 99%

“…Recently, however, several biosynthetic methods suitable for the mass production of TFs have been reported [ 26 , 28 , 29 , 30 ]. Furthermore, a method for producing TFs at the food manufacturing stage has been developed [ 31 , 32 , 33 , 34 , 35 ].…”

Section: Introductionmentioning

confidence: 99%

Synthesis of Theaflavins and Their Functions

Takemoto

2018

Molecules

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Numerous epidemiological and interventional clinical studies have consistently reported that black tea is good for human health. The polyphenolic compound, theaflavin, and its galloyl esters (theaflavins) are the primary red pigments in black tea that possess several health benefits, including fat-reducing and glucose-lowering capabilities and lifestyle-related disease prevention related to anti-obesity, anticancer, anti-atherosclerotic, anti-inflammatory, antiviral, antibacterial, anti-osteoporotic, and anti-dental caries properties. These compounds are produced by key enzymes, such as polyphenol oxidase and peroxidase, from parent green tea catechins present in fresh green tea leaves during the production of black tea leaves or the fermentation of green tea. However, theaflavins are only present in low concentrations in black tea; thus, their extraction from black tea leaves at sufficient levels for use in medical studies has been difficult. To circumvent this issue, different procedures for the synthesis of theaflavins using chemical oxidizing reagents or enzymes have been studied; however, low yields have limited their utility. Recently, however, several biosynthetic methods have been developed for the mass production of theaflavins. Using these methods, the physiological functions of theaflavins in lifestyle-related diseases in mice and humans have also been studied. In this review, we present the synthesis of theaflavins and their health benefits.

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“…2. In our previous report, 4) C. sinensis cell culture have POD activity, and four catechins entirely and selectively converted into TF (1) by POD and hydrolases (Fig. 2).…”

Section: Discussionmentioning

confidence: 72%

“…Their chemical structures [TF (1), TF 3-O-gallate (TF3G) (2), TF 3′-O-gallate (TF3′G) (3), and TF 3,3′-di-O-gallate (TFDG) (4)] are shown below (Fig. 1).…”

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confidence: 99%

A Simple, Enzymatic Biotransformation Method Using Fresh Green Tea Leaves Efficiently Generates Theaflavin-Containing Fermentation Water That Has Potent Physiological Functions in Mice and Humans

Takemoto

Kouno

et al. 2017

Biological & Pharmaceutical Bulletin

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The polyphenolic compound theaflavin, the main red pigment in black tea, possesses many beneficial properties, such as fat-reducing and glucose-lowering capabilities. To produce theaflavin-containing fermentation water on a large scale, we have developed a simple, inexpensive, and selective enzymatic biotransformation method to obtain sufficient levels from fresh green tea leaves. Subsequent administration of theaflavin-containing fermentation water to obese mice on a high-fat diet inhibited body weight gain, decreased casual blood glucose and fasting blood glucose levels, and lowered mesenteric and total fat composition. To note, there were no significant differences observed in food consumption between the experimental and control (water without theaflavin) mice groups. Next, we investigated the effect of this water on blood glucose levels in healthy humans and found that it significantly inhibited blood glucose levels. Thus, we showed that theaflavin-containing fermentation water can be efficiently generated from fresh green tea leaves and demonstrated its significantly potent effects in vivo.

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Theaflavin Synthesized in a Selective, Domino-Type, One-Pot Enzymatic Biotransformation Method with <i>Camellia sinensis</i> Cell Culture Inhibits Weight Gain and Fat Accumulation to High-Fat Diet-Induced Obese Mice

Cited by 17 publications

References 12 publications

Antibacterial Activity of Black Tea Extract against S. mutans, S. aureus, L. acidophilus, Klebsiella and E. coli

Antibacterial Activity of Black Tea Extract against S. mutans, S. aureus, L. acidophilus, Klebsiella and E. coli

Synthesis of Theaflavins and Their Functions

A Simple, Enzymatic Biotransformation Method Using Fresh Green Tea Leaves Efficiently Generates Theaflavin-Containing Fermentation Water That Has Potent Physiological Functions in Mice and Humans

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