KBTBD7, a novel human BTB-kelch protein, activates transcriptional activities of SRE and AP-1

Hu, Jia; Yuan, Wuzhou; Ming, Tang; Wang, Yuequn; Fan, Xiongwei; Mo, Xiaoyang; Li, Yongqing; Ying, Zaochu; Wan, Yongqi; Ocorr, Karen; Bodmer, Rolf; Deng, Yun; Wu, Xiushan

doi:10.5483/bmbrep.2010.43.1.017

Cited by 13 publications

(14 citation statements)

References 23 publications

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“…The BTB/POZ domain is a highly conserved structural motif involved in transcriptional regulation (Aravind and Koonin, ; Collins et al. , ; Hu et al , ). To test the activation of the transcription function of GmBTB/POZ, we performed a transient expression assay in yeast cells using a GAL4‐responsive reporter system.…”

Section: Resultsmentioning

confidence: 99%

GmBTB/POZ, a novel BTB/POZ domain‐containing nuclear protein, positively regulates the response of soybean to Phytophthora sojae infection

Zhang

Gao

et al. 2018

Molecular Plant Pathology

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Phytophthora sojae is a destructive pathogen of soybean [Glycine max (L.) Merr.] which causes stem and root rot on soybean plants worldwide. However, the pathogenesis and molecular mechanism of plant defence responses against P. sojae are largely unclear. Herein, we document the underlying mechanisms and function of a novel BTB/POZ protein, GmBTB/POZ, which contains a BTB/POZ domain found in certain animal transcriptional regulators, in host soybean plants in response to P. sojae. It is located in the cell nucleus and is transcriptionally up-regulated by P. sojae. Overexpression of GmBTB/POZ in soybean resulted in enhanced resistance to P. sojae. The activities and expression levels of enzymatic superoxide dismutase (SOD) and peroxidase (POD) antioxidants were significantly higher in GmBTB/POZ-overexpressing (GmBTB/POZ-OE) transgenic soybean plants than in wild-type (WT) plants treated with sterile water or infected with P. sojae. The transcript levels of defence-associated genes were also higher in overexpressing plants than in WT on infection. Moreover, salicylic acid (SA) levels and the transcript levels of SA biosynthesis-related genes were markedly higher in GmBTB/POZ-OE transgenic soybean than in WT, but there were almost no differences in jasmonic acid (JA) levels or JA biosynthesis-related gene expression between GmBTB/POZ-OE and WT soybean lines. Furthermore, exogenous SA application induced the expression of GmBTB/POZ and inhibited the increase in P. sojae biomass in both WT and GmBTB/POZ-OE transgenic soybean plants. Taken together, these results suggest that GmBTB/POZ plays a positive role in P. sojae resistance and the defence response in soybean via a process that might be dependent on SA.

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Section: Resultsmentioning

confidence: 99%

GmBTB/POZ, a novel BTB/POZ domain‐containing nuclear protein, positively regulates the response of soybean to Phytophthora sojae infection

Zhang

Gao

et al. 2018

Molecular Plant Pathology

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“…It is reported that KBTBD6/7 can mediate ubiquitylation and subsequent proteasomal degradation of T-lymphoma and metastasis gene 1 (TIAM1) through binding cullin 3 (CUL3) to form a heterodimeric Cullin-RING ligases comprising CUL3 (CRL3) complex 30 . KBTBD7 directly or indirectly positively regulates the MAPK signaling pathway through activating SRE and AP-1-mediated transcriptional activation 31 . However, the role of KBTBD7 in cardiovascular diseases remains unclear.…”

Section: Discussionmentioning

confidence: 99%

MicroRNA-21 prevents excessive inflammation and cardiac dysfunction after myocardial infarction through targeting KBTBD7

et al. 2018

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The excessive inflammation triggered by damage-associated molecular patterns (DAMPs) after myocardial infarction (MI) is responsible for the development of cardiac dysfunction and adverse remodeling, while the mechanisms by which inflammation is fine tuned remain to be fully elucidated. MicroRNA-21 (miR-21) has been shown to function in cardiovascular diseases, while its role in inflammatory responses and cardiac function post MI in mice remains unknown. Here, we found that miR-21 expression was markedly increased in border and infarct areas of cardiac tissues during the early inflammatory phase of MI model established by ligating the left-anterior descending coronary artery. MiR-21 knockout mice had decreased survival rates, worse cardiac dysfunction, and increased infarct and scar areas after MI compared with WT mice. MiR-21 knockout mice showed significantly higher levels of inflammatory cytokines including IL-1β, IL-6, and TNF-α in cardiac tissues, as well as infiltration of CD11b+ monocytes/macrophages with higher expression level of inflammatory cytokines. MI induced the great release of high mobility group protein B1 (HMGB1) and heat shock protein 60 (HSP60) in cardiac tissue. MiR-21 deficiency significantly promoted the inflammatory cytokine production triggered by DAMPs in macrophages, whereas, miR-21 overexpression markedly inhibited the inflammatory cytokine production. Mechanistically, miR-21 deficiency enhanced p38 and NF-κB signaling activation in cardiac tissue post MI and macrophages treated with DAMPs. MiR-21 was found to directly target kelch repeat and BTB (POZ) domain containing 7 (KBTBD7), which promoted DAMP-triggered inflammatory responses in macrophages. Furthermore, KBTBD7 interacted with MKK3/6 and promoted their activation, which in turn enhanced the activation of downstream p38 and NF-κB signaling induced by DAMPs. Therefore, our findings demonstrate that miR-21 attenuates inflammation, cardiac dysfunction, and maladaptive remodeling post MI through targeting KBTBD7 and inhibiting p38 and NF-κB signaling activation, suggesting that miR-21 may function as a novel potential therapeutic target for MI.

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“…transcriptional activity when transfected with the control plasmid pCMV-BD encoding the GAL4-DBD alone. LexA-VP16, which can directly activate the expression of the luciferase reporter gene, was used as a positive control (16). When cotransfecting with pL8G5-luc plasmid, the GAL4-ZNF424 fusion protein inhibited luciferase activity by approximately 30% (Fig.…”

Section: Znf424 Functions As a Potential Transcriptional Repressormentioning

confidence: 99%

KBTBD7, a novel human BTB-kelch protein, activates transcriptional activities of SRE and AP-1

Cited by 13 publications

References 23 publications

GmBTB/POZ, a novel BTB/POZ domain‐containing nuclear protein, positively regulates the response of soybean to Phytophthora sojae infection

GmBTB/POZ, a novel BTB/POZ domain‐containing nuclear protein, positively regulates the response of soybean to Phytophthora sojae infection

MicroRNA-21 prevents excessive inflammation and cardiac dysfunction after myocardial infarction through targeting KBTBD7

ZNF424, a novel human KRAB/C2H2 zinc finger protein, suppresses NFAT and p21 pathway

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