Kaz (Anser anser) ve Tavuk (Gallus gallus domesticus) Yumurta Kabuğunun Embriyonik Gelişim Sırasında Taramalı Elektron Mikroskop ile Karşılaştırılması

Abstract

The purpose of this study was to examine the comparative differences of eggshell structure between geese and chickens during embryonic development by SEM. Goose eggs and chicken eggs were used for the study. Eggs of each species were divided into three groups: The eggs within first group were used subsequent to collection, those of second group were collected during the mid-point of incubation and those of third group were collected at the end of the incubation. The eggshells samples with dimensions of 0.5 cm 2 were taken from the equatorial region of eggshells. After these samples were passed through routine process, they were examined via SEM for structural changes of eggshells and rate of elements. In the examination, there were not changes of eggshell structure between two species for days of the incubation. Eggshells thickness of each species and the rate of calcium, oxygen, nitrogen and carbon of eggshells were not statistically different among groups. It was concluded that structural changes of eggshell did not have significant effect on low yield for hatching of goose. Keywords: Calcium, Chicken, Eggshell, Embryo, Goose Kaz (Anser anser) ve Tavuk (Gallus gallus domesticus) Yumurta Kabuğunun Embriyonik Gelişim Sırasında Taramalı Elektron Mikroskop ile Karşılaştırılması ÖzetBu çalışmada, taramalı electron mikroskop kullanılarak embriyonik gelişim sırasında kaz ve tavukların yumurta kabuğundaki değişiklikleri karşılaştırmak amaçlanmıştır. Bu çalışmada kaz ve tavuk yumurtası kullanılmıştır. Her iki türe ait yumurtalar üç gruba ayrıldı. Birinci gruba ait yumurta kabukları kuluçkaya konulmadan, ikinci gruba ait yumurta kabukları kuluçka süresinin ortasında ve üçüncü gruba ait olanlar ise kuluçkanın son günü olan çıkım gününde toplandı. Her bir yumurta kabuğunun ekvator kısmından 0,5 cm 2 ebatında örnekler alındı. Bu örnekler rutin prosedürlerden geçirildikten sonra taramalı elektron mikroskobunda yapısal değişiklikler ve element oranı bakımından incelendi. Elektron mikroskop incelemesinde, kaz ve tavuk türlerinin kuluçka günlerine bağlı olarak yumurta kabuğu katmanlarında belirgin bir değişikliğin olmadığı tespit edildi. Her iki türün kendi içinde kuluçka günlerine göre yumurta kalınlığında ve kabuklardaki elementlerin oranlarında istatistiksel olarak benzerlik olduğu görüldü. Yumurta kabuğunun embriyonik gelişim boyunca uğradığı yapısal değişikliklerin kaz kuluçka veriminin düşük olmasında önemli bir etkiye sahip olmadığı sonucuna varıldı.

A Study on the Effect of Altitude on Shell Thickness in Goose Eggs

“…2016). There was no significant change in shell thickness during incubation in geese and hens (Bingöl et al 2016, Yildirim 2018. No significant change in the shell thickness of 1year-old geese was found when compared to other ages (Tilki and İnal 2004).…”

Comparison of the eggshell mammillary microstructure of Galliformes and Anseriformes

“…The observations of Bingol et al. (2016) were quite cursory and showed no differences between the eggshell microstructure and ultrastructure of chicken and goose. Other, more detailed studies of eggshell structure (including the orientation of calcium carbonate crystals) in guinea fowl and geese showed significant differences between these species (Pérez‐Huerta & Dauphin, 2016).…”

“…The research of avian eggshells microstructure and ultrastructure is mainly concerned with domesticated birds (Bingol et al, 2016;P erez-Huerta & Dauphin, 2016). The observations of Bingol et al (2016) were quite cursory and showed no differences between the eggshell microstructure and ultrastructure of chicken and goose.…”

“…The research of avian eggshells microstructure and ultrastructure is mainly concerned with domesticated birds (Bingol et al, 2016;P erez-Huerta & Dauphin, 2016). The observations of Bingol et al (2016) were quite cursory and showed no differences between the eggshell microstructure and ultrastructure of chicken and goose. Other, more detailed studies of eggshell structure (including the orientation of calcium carbonate crystals) in guinea fowl and geese showed significant differences Dauphin, 2016).…”

“…The research of avian eggshells microstructure and ultrastructure is mainly concerned with domesticated birds (Bingol et al., 2016; Pérez‐Huerta & Dauphin, 2016). The observations of Bingol et al.…”