Karyotyping and single-gene detection using fluorescence in situ hybridization on chromosomes of Hydra magnipapillata (Cnidaria: Hydrozoa)

Anokhin, Boris A.; Hemmrich-Stanisak, Georg; Bosch, Thomas C. G.

doi:10.3897/compcytogen.v4i2.41

Cited by 16 publications

(16 citation statements)

References 32 publications

(32 reference statements)

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“…Previous scattered experimental evidence showed that Nematostella is a diploid animal with chromosome number 2n = 30 [ 34 ]. Interestingly, this number is identical to those of several other hydrozoans and it is also comparable to those of several species of Acropora corals (2n = 28) [ 50 , 51 ]. Utilizing the karyotyping method discussed above on early stage embryos (64- and 128-cell stage) and optimizing a few steps, we easily obtained highly synchronized metaphase spreads.…”

Section: Discussionsupporting

confidence: 73%

An adaptable chromosome preparation methodology for use in invertebrate research organisms

Guo

Accorsi

et al. 2018

BMC Biol

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BackgroundThe ability to efficiently visualize and manipulate chromosomes is fundamental to understanding the genome architecture of organisms. Conventional chromosome preparation protocols developed for mammalian cells and those relying on species-specific conditions are not suitable for many invertebrates. Hence, a simple and inexpensive chromosome preparation protocol, adaptable to multiple invertebrate species, is needed.ResultsWe optimized a chromosome preparation protocol and applied it to several planarian species (phylum Platyhelminthes), the freshwater apple snail Pomacea canaliculata (phylum Mollusca), and the starlet sea anemone Nematostella vectensis (phylum Cnidaria). We demonstrated that both mitotically active adult tissues and embryos can be used as sources of metaphase chromosomes, expanding the potential use of this technique to invertebrates lacking cell lines and/or with limited access to the complete life cycle. Simple hypotonic treatment with deionized water was sufficient for karyotyping; growing cells in culture was not necessary. The obtained karyotypes allowed the identification of differences in ploidy and chromosome architecture among otherwise morphologically indistinguishable organisms, as in the case of a mixed population of planarians collected in the wild. Furthermore, we showed that in all tested organisms representing three different phyla this protocol could be effectively coupled with downstream applications, such as chromosome fluorescent in situ hybridization.ConclusionsOur simple and inexpensive chromosome preparation protocol can be readily adapted to new invertebrate research organisms to accelerate the discovery of novel genomic patterns across the branches of the tree of life.

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Section: Discussionsupporting

confidence: 73%

An adaptable chromosome preparation methodology for use in invertebrate research organisms

Guo

Accorsi

et al. 2018

BMC Biol

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“…Molecular or cellular signals for the sex determination and reversal, however, are unknown. The karyotype of Hydra magnipapillata chromosomes did not reveal any heteromorphic chromosome pair which could be responsible for the determination of the sex as it is in higher metazoans (Anokhin et al 2010). …”

Section: The Cnidarian Sc: Hydra As Model System For Meiosis Research?mentioning

confidence: 87%

Evolutionary history of the mammalian synaptonemal complex

et al. 2016

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“…It allows differentiation between chromosomes very similar in size that could not be distinguished by conventional banding [Kato et al, 2005;Anokhin et al, 2010;Cioffi et al, 2010].…”

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confidence: 99%

Chromosomal Localization of Ribosomal and Telomeric DNA Provides New Insights on the Evolution of Gomphocerinae Grasshoppers

Jetybayev

Bugrov²,

Карамышева³

et al. 2012

Cytogenet Genome Res

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Chromosome location of ribosomal DNA (rDNA) and telomeric repeats was analysed in mitotic chromosomes of 15 species of Gomphocerinae grasshoppers belonging to the tribes Arcypterini, Gomphocerini, Stenobothrini, and Chrysochraontini. Two types of rDNA distribution were found in the Gomphocerini tribe. Type 1, found in 9 species, was characterized by the presence of rDNA in the short arm of the long biarmed chromosomes 2 and 3 and, in some species, also in the X chromosome. Type 2 was found only in Aeropus sibiricus and Stauroderus scalaris and consisted in the presence of pericentromeric rDNA blocks in all chromosomes. A comparison of rDNA distribution in Gomphocerini species with 2n♂ = 23, 2n♂ = 21, and 2n♂ = 17 suggested the possible involvement of chromosome 6 in the ancestral karyotype (2n♂ = 23) in 1 of the 3 centric fusions that decreased the chromosome number in these species. In the tribe Stenobothrini, Stenobothrus eurasius carried a single rDNA cluster in the X chromosome, likewise 2 Spanish species previously analysed, but Omocestus viridulus unusually showed a single rDNA cluster in the longest autosome. Telomeric repeats were located primarily on the ends of chromosome arms. In 2 species, however, we observed the presence of interstitial clusters outside telomeric regions. The first one, Aeropus sibiricus, exhibited a polymorphic interstitial site of telomeric repeats in chromosome 6 as a consequence of a paracentric inversion. Most remarkably, Chorthippus jacobsoni showed the presence of telomeric repeats in the pericentric regions of the 3 biarmed chromosome pairs originated by centric fusion, thus suggesting that these rearrangements were not of the Robertsonian type but true centric fusion with a probable generation of dicentric chromosomes.

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Karyotyping and single-gene detection using fluorescence in situ hybridization on chromosomes of Hydra magnipapillata (Cnidaria: Hydrozoa)

Cited by 16 publications

References 32 publications

An adaptable chromosome preparation methodology for use in invertebrate research organisms

An adaptable chromosome preparation methodology for use in invertebrate research organisms

Evolutionary history of the mammalian synaptonemal complex

Chromosomal Localization of Ribosomal and Telomeric DNA Provides New Insights on the Evolution of Gomphocerinae Grasshoppers

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