Chromosomal Localization of Ribosomal and Telomeric DNA Provides New Insights on the Evolution of Gomphocerinae Grasshoppers

Jetybayev, I. E.; Bugrov, Alexander G.; Карамышева, Т. В.; Camacho, Juan Pedro M.; Rubtsov, N. B.

doi:10.1159/000341571

Cited by 18 publications

(31 citation statements)

References 51 publications

(37 reference statements)

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“…DNA amplification involving rDNA also took place in the formation of C-positive blocks in the distal part of the long arms of the M 8 and S 9 chromosomes. It is should be noted that usually rDNA amplification or enrichment of some chromosome regions by rDNA leads to its heterochromatization (Bugrov et al 2003, Jetybayev et al 2012). We observed this phenomenon in the distal part of the long arms of the M 8 and S 9 chromosomes but short arms of the chromosomes remained C-negative.…”

Section: Discussionmentioning

confidence: 99%

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Sex chromosome diversity in Armenian toad grasshoppers (Orthoptera, Acridoidea, Pamphagidae)

Bugrov¹,

Jetybayev²,

Karagyan³

et al. 2016

CCG

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Although previous cytogenetic analysis of Pamphagidae grasshoppers pointed to considerable karyotype uniformity among most of the species in the family, our study of species from Armenia has discovered other, previously unknown karyotypes, differing from the standard for Pamphagidae mainly in having unusual sets of sex chromosomes. Asiotmethis turritus (Fischer von Waldheim, 1833), Paranocaracris rubripes (Fischer von Waldheim, 1846), and Nocaracris cyanipes (Fischer von Waldheim, 1846) were found to have the karyotype 2n♂=16+neo-XY and 2n♀=16+neo-XX, the neo-X chromosome being the result of centromeric fusion of an ancient acrocentric X chromosome and a large acrocentric autosome. The karyotype of Paranothrotes opacus (Brunner von Wattenwyl, 1882) was found to be 2n♂=14+X1X2Y and 2n♀=14+X1X1X2X2., the result of an additional chromosome rearrangement involving translocation of the neo-Y and another large autosome. Furthermore, evolution of the sex chromosomes in these species has involved different variants of heterochromatinization and miniaturization of the neo-Y. The karyotype of Eremopeza festiva (Saussure, 1884), in turn, appeared to have the standard sex determination system described earlier for Pamphagidae grasshoppers, 2n♂=18+X0 and 2n♀=18+XX, but all the chromosomes of this species were found to have small second C-positive arms. Using (FISH)fluorescent in situ hybridization with 18S rDNA and telomeric (TTAGG)n DNA repeats to yield new data on the structural organization of chromosomes in the species studied, we found that for most of them, clusters of repeats homologous to 18S rDNA localize on two, three or four pairs of autosomes and on the X. In Eremopeza festiva, however, FISH with labelled 18S rDNA painted C-positive regions of all autosomes and the X chromosome; clusters of telomeric repeats localized primarily on the ends of the chromosome arms. Overall, we conclude that the different stages of neo-Y degradation revealed in the Pamphagidae species studied make the family a very promising and useful model for studying sex chromosome evolution.

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Section: Discussionmentioning

confidence: 99%

“…Three clusters of rDNA on one chromosome were previously reported for Pamphagus ortolaniae (Vitturi et al 2008). Multiple localizations of rDNA clusters in a chromosome is a very rare type of rDNA cluster distribution (Cabrero and Camacho 2008, Jetybayev et al 2012, Palacios-Gimenez et al 2013). …”

Section: Discussionmentioning

confidence: 99%

Sex chromosome diversity in Armenian toad grasshoppers (Orthoptera, Acridoidea, Pamphagidae)

Bugrov¹,

Jetybayev²,

Karagyan³

et al. 2016

CCG

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“…Nguyen et al 2010, Vershinina et al 2015) hemipterans (Kuznetsova et al, 2015, Maryańska-Nadachowska et al 2016), and orthopterans (grasshoppers e.g. Cabrero et al 2009, Cabral-de-Mello 2011b, Jetybayev et al 2012, Neto et al 2013). Recently, a series of works using advanced, such as fluorescence in situ hybridization (FISH), and conventional chromosome banding techniques, showed that the number and location of rDNA (NOR) and heterochromatin, respectively, can be useful markers for the studying the karyotypes evolution in tettigoniids, and for the identification of genus/species-specific patterns, namely in the subfamilies Phaneropterinae (e.g.…”

Section: Introductionmentioning

confidence: 99%

Comparative analysis of chromosomes in the Palaearctic bush-crickets of tribe Pholidopterini (Orthoptera, Tettigoniinae)

Warchałowska‐Śliwa¹,

Grzywacz²,

Heller³

et al. 2017

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The present study focused on the evolution of the karyotype in four genera of the tribe Pholidopterini: Eupholidoptera Mařan, 1953, Parapholidoptera Mařan, 1953, Pholidoptera Wesmaël, 1838, Uvarovistia Mařan, 1953. Chromosomes were analyzed using fluorescence in situ hybridization (FISH) with 18S rDNA and (TTAGG)n telomeric probes, and classical techniques, such as C-banding, silver impregnation and fluorochrome DAPI/CMA3 staining. Most species retained the ancestral diploid chromosome number 2n = 31 (male) or 32 (female), while some of the taxa, especially a group of species within genus Pholidoptera, evolved a reduced chromosome number 2n = 29. All species show the same sex determination system X0/XX. In some taxa, a pericentric inversion has changed the morphology of the ancestral acrocentric X chromosome to the biarmed X. The rDNA loci coincided with active NORs and C-band/CG-rich segments. A comparison of the location of the single rDNA/NOR in the genus Pholidoptera suggests that reduced chromosome number results from Robertsonian translocation between two pairs of autosomes, one carrying the rDNA/NOR. The results constitute a step towards better understanding of the chromosomal reorganization and evolution within the tribe Phaneropterini and the whole subfamily Tettigoniinae.

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“…In the subfamily Gomphocerinae, previous studies using FISH analysis of 38 grasshopper species showed that the 45S rDNA sequence is present on more than one chromosome pair in 71% of cases Jetybayev et al, 2012). In contrast, studies of 5S rDNA sites in 14 species showed that they are present on more than one chromosome pair in 99.8% of cases; the only exceptions were Chorthippus apicalis and Stauroderus scalaris in which signals are limited to chromosome pairs 6 and 3, respectively (Loreto et al, 2008;Cabral-de-Mello et al, 2011a).…”

Section: Introductionmentioning

confidence: 91%

“…Molecular cytogenetic studies using fluorescence in situ hybridization (FISH) with repetitive DNA probes, such as rDNA and histone genes, have been carried out on many grasshopper species, particularly of the family Acrididae Loreto et al, 2008;Cabrero et al, 2009;Cabral-de-Mello et al, 2011a;Oliveira et al, 2011;Rocha et al, 2011;Jetybayev et al, 2012;Bueno et al, 2013;Palacios-Gimenez et al, 2013). The objective of physical mapping of these and other repetitive DNA sequences is to provide information on chromosome structure and gene distributions in a range of species that would serve as a basis for phylogenetic studies Cabrero et al, 2009;Cabral-de-Mello et al, 2011a,b;Jetybayev et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Physical mapping of 18S and 5S rDNA loci and histone H3 gene in grasshopper species of the subfamily Gomphocerinae (Acrididae)

Silva-Neto¹,

Bernardino²,

Loreto³

et al. 2015

Genet. Mol. Res.

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ABSTRACT. In this study, fluorescence in situ hybridization (FISH) analysis was used to determine and compare the numbers and chromosomal locations of two multigene families (rDNA and histone H3) in four Neotropical species of gomphocerine grasshoppers. FISH using the 18S rDNA probe identified a single site on the S9 chromosome of Amblytropidia sp and Cauratettix borelli, a single site on chromosome M6 of Compsacris pulcher, and two sites (chromosomes L1 and L2) in Orphulella punctata. By contrast, FISH with a 5S rDNA probe identified dispersion of this sequence in the genomes of the four species, with evidence of intraspecific variations. Amblytropidia sp had six to eight FISH signals on autosomal chromosomes, while C. pulcher exhibited a signal only on the M5 bivalent. The histone H3 gene was less variable and was restricted to a single pair in all species. The conservation of the numbers and locations of 18S rDNA and H3 genes in conjunction with data from the literature was useful 15009 Physical mapping in Gomphocerinae species ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 14 (4): 15008-15015 (2015) for evaluating karyotype evolution in this subfamily. The variation in the number and sizes of 5S rDNA sites indicates a process of recent dispersion that might have been mediated by transposition.

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Chromosomal Localization of Ribosomal and Telomeric DNA Provides New Insights on the Evolution of Gomphocerinae Grasshoppers

Cited by 18 publications

References 51 publications

Sex chromosome diversity in Armenian toad grasshoppers (Orthoptera, Acridoidea, Pamphagidae)

Sex chromosome diversity in Armenian toad grasshoppers (Orthoptera, Acridoidea, Pamphagidae)

Comparative analysis of chromosomes in the Palaearctic bush-crickets of tribe Pholidopterini (Orthoptera, Tettigoniinae)

Physical mapping of 18S and 5S rDNA loci and histone H3 gene in grasshopper species of the subfamily Gomphocerinae (Acrididae)

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