Karyotypic studies of four Physalis species from Nigeria

Azeez, Sekinat Okikiola; Faluyi, Julius Olaoye

doi:10.1556/034.61.2019.1-2.2

Cited by 4 publications

(1 citation statement)

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“…Most cytogenetic studies in Physalis peruviana L. have used classical staining techniques to determine the chromosome number, where 2n=48 predominates (Rodríguez and Bueno 2006;Sánchez 2014;Liberato et al 2014;Trevisani et al 2018), while other studies report mixoploid plants (Sánchez 2014), and somatic aneuploidy (Carbajal et al 2021). Karyotypic formulas of some populations have been previously reported (Azeez and Faluyi 2019;Carbajal et al 2021). However, since goldenberry chromosomes are small, numerous, and have similar morphology, the karyotype determination and its analysis are considered limited when classical staining techniques are used.…”

Section: Introductionmentioning

confidence: 99%

Nuclear DNA content and comparative FISH mapping of the 5s and 45s rDNA in wild and cultivated populations of Physalis peruviana L.

et al. 2023

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Physalis peruviana L. often known as goldenberry, has increased its commercial growth in the international market in recent years due to its nutritional value and antioxidant potential. This situation has enabled countries such as Peru to increase their production in order to meet the global demand. However, investigations about the genetic diversity of cultivated and wild populations of goldenberry are still in their early stages. FISH mapping of 5s and 45s rDNA loci and flow cytometry estimation of nuclear DNA content were used to assess genetic differences between wild and cultivated goldenberry populations from Ayacucho and Cajamarca. The majority of metaphases had six 5s rDNA sites for all populations and two and four 45s rDNA sites for the cultivated and wild populations, respectively. We were able to characterize nine different types of chromosomes based on their morphology, fluorescence, rDNA location, and conservation across populations by analyzing the chromosomes that contained rDNA. Furthermore, cultivated populations had more nuclear DNA (13.262±0.087 pg) than wild populations (12.955±0.086 pg). The results show genetic differences between wild and cultivated populations of goldenberry at molecular cytogenetic level as well as in genome size. These findings establish a precedent for future cytogenetic and genomic studies in goldenberry populations, enabling future breeding programs.

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