Karyotypic and Nuclear DNA Variations inLathyrus sativus(Fabaceae)

Abstract

Karyotypic studies were performed on 20 grasspea (Lathyrus sativus L.) genotypes (15 genotypes from ICARDA and 5 from Iran) labeled G1-G20. All genotypes examined were diploid (2n = 2x = 14), having mean chromosome length (TL) of 6.03 µm (4.7-6.7 µm) and total chromosome volume mean (TCV) of 24.2 µm 3 (18.1-35.1 µm 3). The largest value of chromatin length was detected on G8 (93.3 µm) while G3 demonstrated the smallest value (65.2 µm). Karyotype formula was determined as "14m" in 19 genotypes and "12m + 2sm" in G20, using LEVAN et al. chromosome nomenclature. Satellite numbers varied between 1 to 2 pairs. Karyotypes were classifi ed as 1A of STEBBINS classifi cation. The scatter diagram of A1 and A2 asymmetry indicated 5 groups of genotypes: G2 and G19 with the most asymmetrical and symmetrical karyotypes, respectively. Results of multivariate analysis and scatter diagram of TL with CI indicated 3 groups of genotypes according to their chromosomal parameters. Flow cytometric studies were performed, using PI staining method and Pisum sativum cv. Ctirad (2C DNA = 9.09 pg) as a reference standard. The mean 2C DNA content of all genotypes was 14.7 pg, ranging from 13.8 pg (G1) to 15.6 pg (G20). Signifi cant positive correlations were found between 2C DNA content and either stomatal cell area or longitude.

Mutagenic Effectivity of Cadmium Sulphide and Copper Oxide Nanoparticles on Some Physiological and Cytological Attributes of <i>Lathyrus sativus</i> L.

“…Cells with paired fragments are notably observed in both metaphase and anaphase. Ghasem et al (2011) performed karyotype analysis of different germplasms of L. sativus and reported mostly one and rarely two pairs of chromosomes with satellites. In the present study, paired fragments ( Fig.…”

Genetic Diversity and Similarity of Asian and European Pears (Pyrus Spp.) Revealed by Genome Size and Morphological Traits Prediction

“…We found a linear relationship between genome size with traits such as fruit diameter, fruit size, fruit pedicel length, and length-to-diameter ratio. Previous studies also showed a significant relationship between genome size and some phenotypic traits as well as geographical locations in different plant species, such as Lathyrus sativus (Karimzadeh et al, 2011), Diphasiastrum sp. (Hanusova et al, 2014), and Iranian endemic muskmelon (Karimzadeh et al, 2010).…”

“…In the present research, we employed cytological studies to detect the differences within the species and between the studied pear species. It has been reported that cytology studies are important in the populations of one species due to their differences, each one exhibits a particular genomic that are in agreement with the environment in which they are being used (Karimzadeh et al, 2010;Karimzadeh et al, 2011;Abedi et al, 2015). Flow cytometry can be employed to determine ploidy levels, estimate the size of the genome in plants, and examine the cell cycle in humans and animals (Doležel and Bartoš, 2005;Doležel et al, 2007).…”

“…Flow cytometry can be employed to determine ploidy levels, estimate the size of the genome in plants, and examine the cell cycle in humans and animals (Doležel and Bartoš, 2005;Doležel et al, 2007). Also, the application of flow cytometry to detect the polyploidy induction and its effects on morphology, physiology, anatomy, and secondary metabolites have been reported on a wide variety of different plants (Majdi et al, 2010, Karimzadeh et al, 2011Abedi et al, 2015;Tavan et al, 2015;Javadian et al, 2017). Genetic diversity is assessed by various factors such as phenotypes, and biochemical, cytological, and molecular characteristics (Govindaraj et al, 2015).…”

Cytogenetics of a reciprocal translocation integrating distichous pedicel and tendril-less leaf mutations inLathyrus sativusL.

“…Grass pea (Lathyrus sativus L.) is an annual leguminous crop with 2n = 2x = 14 chromosomes in its somatic cells (Lavania 1982;Talukdar and Biswas 2007;Ghasem et al 2011). The flowers are typically papilionaceous, and like in many other legumes, develop on the axillary racemose inflorescence by a peduncle which is divided into two parts from an arista.…”