Karyotypic analysis of different populations of Carthamus tinctorius Linnaeus (Asteraceae)

Malik, Anjali; Srivastava, Anand

doi:10.3897/compcytogen.v3i1.8

Cited by 6 publications

(4 citation statements)

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“…Mostly, metacentric and submetacentric chromosomes with variable karyotypic symmetry were reported in species of Passiflora [8]. Primitive nature of the species due to the predominance of metacentric chromosomes was suggested in Carthamus tinctorius [18]. Karyotype analysis revealed significant variations in TF% within the species of P. foetida.…”

Section: Discussionmentioning

confidence: 92%

“…Significance of seed storage proteins in cultivar differentiation was suggested in species like Cucumis melo and Citrullus lanatus [32] and in Oryza sativa [27]. Karyotype analysis had also been successfully employed in assessing taxonomic relations in the species of Boronia [25], Carthamus tinctorius [18,34], Prunus [14] and in Allium [20]. The technique has been proved useful at intra-specific levels too [19,33,35].…”

Section: Introductionmentioning

confidence: 99%

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Genetic diversity in two species of Passiflora L. (Passifloraceae) by karyotype and protein profiling

Beena

Beevy

2015

Nucleus

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Karyotype and SDS-PAGE analysis in three accessions each of Passiflora edulis; P. edulis Sims. f. flaviocarpa O.Deg -yellow passion fruit, P.edulis f. edulis -purple passion fruit, and P.edulis 'Panama Red', and in three varieties of the wild species, P. foetida, P.foetida var. foetida, P.foetida var. hispida, and P. foetida var. gossippifolia, were carried out to study the genetic diversity in the species. Analysis of root tip cells revealed the presence of 2n=18 in P.edulis, 2n=16 in P.foetida var. foetida, and 2n=20 in P.foetida var. hispida and P.foetida var. gossippifolia. The somatic chromosome number of two varieties viz, P.foetida var. hispida, and P. foetida var. gossippifolia are reported for the first time. Karyotype analysis and SDS-PAGE protein profiling suggest variations at the intra-as well as interspecific levels in Passiflora. Low TF% i.e., 40 % observed in varieties of P. edulis point to the evolved nature of the cultivated taxa. Unique protein bands in P. foetida (65.66KDa) and P.edulis (17.50KDa) support their interspecific variation. Dendrogram based on UPGMA analysis emphasize the relationships among the taxa.

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Section: Discussionmentioning

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Genetic diversity in two species of Passiflora L. (Passifloraceae) by karyotype and protein profiling

Beena

Beevy

2015

Nucleus

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“…In ancient and primordial plants, symmetric karyotype was found to be predominant, while the asymmetric karyotype was mostly seen in the recent and specialised plants (Jiang et al 2011). Therefore, asymmetric karyotypes are more advanced compared to symmetrical ones (Malik and Srivastava 2009). Symmetric karyotypes were reported by Laura et al (2010) for Lycium species, which were mostly metacentric chromosomes with slight differences in size.…”

Section: Bmentioning

confidence: 95%

Karyotypic studies of four Physalis species from Nigeria

Azeez

Faluyi

2019

Acta Botanica Hungarica

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Mitotic chromosomes from root tips of four Nigerian Physalis species were investigated using standard cytogenetic methods. P. angulata has chromosome number of 2n = 48 with karyotypic formula of 2M + 5m + 16sm + 1st, while P. micrantha, P. peruviana and P. pubescens showed the same chromosome number of 2n = 24 with karyotypic formulae of 1M + 1m + 9sm + 1st, 4M + 6m + 1sm + 1st and 1M + 1 m + 2sm + 8st, respectively. The karyotypes show that P. angulata is more advanced when compared to the other three diploids studied.

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“…It was the confirmation of diploid gamete. Malik and Srivastava (2009) demonstrated that treatment with colchicine in Carthamus incited appreciable changes in the diameter of pollen grains, decreased the number of pollen grains per anther and increased pollen sterility. Bragdo (1955) recorded that in the tetraploid of red clover, there was a much greater variation in pollen size and fertility.…”

Section: Discussionmentioning

confidence: 99%

Induced Colchitetraploids in <i>Allium cepa</i> L.

2015

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SummaryPolyploidy was induced by colchicine in Allium cepa L. Five plants of Allium cepa (AC1-AC5) were isolated from the 0.2% colchicine treated inflorescences. The meiotic stages were analyzed in treated buds. Various cytological features like chromosomal associations (quadrivalents, bivalents and univalents), chiasmata frequency and terminalization coefficient were recorded at diakinesis/metaphase-I. Anaphase/telophase-I/II were studied in all the cholchitetraploids. In all these plants, the anaphase/telophase-I were abnormal showing unequal distribution, laggards, micronuclei and multipolar at telophase-II. Pollen mitosis was studied in these plants. The chromosomes (n=16) were found at metaphase. Pollen fertility was low (40.9 to 56.9%) in all colchitetraploids.Key words Induced colchitetraploid, Allium cepa L., Pollen fertility, Colchicine, Chromosome.The importance of polyploid plants in agriculture is well known. Colchicine has been largely used to alter ploidy level frequencies in cell populations to obtain tetraploid plants, starting with diploid material. In fact, polyploid plants usually have larger and thicker leaves, stems or roots, and also flowers, fruits and seeds, and then, a greater yield (Gao et al. 1996).The genus Allium consists of 700 species in the world (Friesen et al. 2005). Allium cepa L. is one of the most important plant species which was cultivated thousands of years ago as an edible plant (Jamila et al. 2009). Its chromosome number is low (2n=16), and it has a relatively large size and is susceptible to cytological manipulations (Mercykutty and Stephen 1980). The present investigation was planned to induce polyploidy in Allium cepa by colchicine treatment and identify the ploidy level by counting the chromosome number. Materials and methods Colchicine treatmentYoung inflorescences were treated with 0.2% colchicine solution for 6, 12, 18, or 24 h also by using the cotton swab method . After treatment inflorescences were thoroughly washed with water. Study of meiosisFor meiotic studies, young flower buds of appropriate size were collected between 10 to 11 AM and fixed in freshly prepared Carnoy s fluid (absolute alcohol and acetic acid in 3 : 1 ratio). Anthers were separated, teased in a drop of 2% iron acetocarmine on a clean slide and squashed under a cover glass. Pollen mother cells (PMCs) were analyzed for suitable stages of meiosis. Temporary slides were analyzed for different stages of meiosis like diakinesis, metaphase-I, anaphase-I, telophase-I and -II. Chromosomal associations, chiasmata frequency, distribution of chromosomes and other features were recorded. Chiasma frequency was calculated according to Ward (1979).

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Karyotypic analysis of different populations of Carthamus tinctorius Linnaeus (Asteraceae)

Cited by 6 publications

References 5 publications

Genetic diversity in two species of Passiflora L. (Passifloraceae) by karyotype and protein profiling

Genetic diversity in two species of Passiflora L. (Passifloraceae) by karyotype and protein profiling

Karyotypic studies of four Physalis species from Nigeria

Induced Colchitetraploids in <i>Allium cepa</i> L.

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