Karyotype, ribosomal genes, and telomeric sequences in the crayfish Procambarus clarkii (Decapoda: Cambaridae)

Salvadori, Susanna; Coluccia, Elisabetta; Deidda, Federica; Cau, Angelo; Cannas, Rita; Lobina, Cinzia; Sabatini, Andrea; Deiana, Anna Maria

doi:10.1163/1937240x-00002247

Cited by 12 publications

(12 citation statements)

References 24 publications

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“…However, some evidence supports the hypothesis that the chromosome evolution within the genus could involve several fusion events giving rise to a reduction on the chromosome number in P. serratus : i) We observed interstitial DAPI-bright bands on the large metacentric chromosomes of P. serratus , being DAPI-positive bands that are characteristic of centromeric regions in both Palaemon species, as observed in other families of decapods such as Astacidae [23, 24], Cambaridae [25], Nephropidae [26], Scyllaridae [27] and Palinuridae [28]. ii) The presence of interstitial C-bands on these chromosomes may represent a chromosome fusion event.…”

Section: Discussionsupporting

confidence: 80%

Comparative cytogenetic analysis of marine Palaemon species reveals a X1X1X2X2/X1X2Y sex chromosome system in Palaemon elegans

et al. 2017

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BackgroundThe maintenance of species and the promotion of speciation are closely related to chromosomal rearrangements throughout evolution. Decapoda represents the most species-rich order among crustaceans and, despite its ecological and economic importance, little is known about decapod karyology. We aim at cytogenetically characterizing two sympatric prawn species.ResultsAnalysis of mitotic metaphases and meiotic diakinesis of the common prawn Palaemon serratus and the rockpool prawn P. elegans, revealed considerable differences between their karyotypes including chromosome numbers and sex determination systems. The cytogenetic data for P. serratus showed a diploid number of 56 and the putative absence of heteromorphic sex chromosomes. However, the diploid chromosome number in P. elegans was 90 for females and 89 for males. The karyotype of the females consisted of the three largest acrocentric pairs and 42 submetacentric and metacentric pairs, while the karyotype of the males comprised a clearly identifiable large metacentric chromosome and two acrocentric pairs as well as the smaller 42 pairs. These results highlight the presence of the X1X1X2X2/X1X2Y multiple sex chromosome system in P. elegans, which constitute the only sexual system for Decapoda reported cytogenetically using modern techniques. The origin of this sex chromosome system is discussed. We hypothesize that the chromosome evolution within the genus could involve several fusion events giving rise to a reduction on the chromosome number in P. serratus. In both species, the major ribosomal genes were located in two chromosome pairs and hybridization signals of the telomeric sequences (TTAGGG)n were visualized at the telomeres of all chromosomes. C-banding revealed that, when present, constitutive heterochromatin had a predominantly telomeric distribution and no centromeric constitutive heterochromatin was observed.ConclusionsAlthough more comparative cytogenetic analyses are needed to clarify our hypotheses, the findings of this work indicate that the prawns of the genus Palaemon represent a promising model among Decapoda representatives to investigate the karyotype evolution and the patterns of sex chromosome differentiation.

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Section: Discussionsupporting

confidence: 80%

Comparative cytogenetic analysis of marine Palaemon species reveals a X1X1X2X2/X1X2Y sex chromosome system in Palaemon elegans

et al. 2017

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“…FISH experiments were carried out according to Salvadori et al (2014) using a 45S rDNA probe, obtained from a complete major ribosomal unit of Xenopus laevis, and a non-templated polymerase chain reaction (PCR)-generated (TTAGG)n probe, according to Ijdo et al (2001). The probes were labelled with biotin-16-dUTP (Roche Diagnostic, Milan, Italy) or digoxigenin-11-dUTP (Roche Diagnostic) using a Nick Translation Kit (Roche Diagnostic); the detection of FISH signals was carried out using fluorescein-conjugated avidin (Vector Labs., Burlingame, CA, USA) and antidigoxigenin-rhodamine (Sigma-Aldrich, Milan, Italy), respectively.…”

Section: Methodsmentioning

confidence: 99%

Co-localization of ribosomal and telomeric sequences in Leptynia (Insecta: Phasmatodea)

Scali

Coluccia

Deidda

et al. 2016

Italian Journal of Zoology

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Stick insects have been studied mainly for non-conventional reproduction modes, such as parthenogenesis, hybridogenesis and androgenesis. Parallel karyological investigations have evidenced extensive numerical and structural chromosome re-patterning, particularly evident in hybrid parthenogenetic taxa. Chromosome sets of bisexual Leptynia (Pantel) species show an evolutionary trend from 40 to 36 chromosomes and are characterized by cytological satellites of variable size and localization. We performed fluorescence in situ hybridization (FISH) analysis using 45S ribosomal genes and pentameric (TTAGG)n telomere sequences as probes in two strictly related but karyotypically distinct species, L. montana Scali (2n = 38/37; XX/XO) and L. attenuata Pantel (2n = 36). L. attenuata has recently been split into three subspecies (L. attenuata attenuata, L. attenuata iberica and L. attenuata algarvica), and found to share an XX/XY sex chromosome formula, unusual for stick insects. FISH by 45S rDNA sequences consistently labelled the short arm of the 4th chromosome pair, often of a variable size. Silver staining showed that nucleolar organizer regions (NORs) are active. FISH of the telomeric repeats, besides ordinary telomeres, also labelled the short arm of this same pair. The use of both probes in double FISH analysis fully confirmed the co-localization of ribosomal and telomeric highly repeated sequences. Since it is increasingly emerging that the co-localization of NORs and telomeric sequences appears to be a feature shared by evolutionarily distant animals, its possible role is discussed.

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“…Telomeres are highly repeated DNA sequences that protect the ends of the chromosomes [63] and show striking similarity even in evolutionarily distant organisms [64]. In several crustacean groups discernible telomeric signals have been reached using as (TTAGGG)n as (TTAGG)n probes [65,66]. However, in Macrobrachium species the FISH experiments with (TTAGGG)n probes did not produce detectable signals.…”

Section: Discussionmentioning

confidence: 99%

“…Another striking characteristic in Macrobrachium species is their large content of AT-rich segments in the centromeric regions. However, this pattern has a broader phylogenetic extension, including other species of the genus [34], other Palaemonidae genera [31], and Decapoda families [66], thus indicating a stable condition for the order.…”

Section: Discussionmentioning

confidence: 99%

Molecular Cytogenetic Analysis in Freshwater Prawns of the Genus Macrobrachium (Crustacea: Decapoda: Palaemonidae)

Molina

Costa

Cunha

et al. 2020

IJMS

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Freshwater prawns of the genus Macrobrachium are one of the important components of circumtropical marine, estuarine, and freshwater environments. They have been extensively exploited for human consumption for many years. More than 250 species reflect the evolutionary success of this highly diversified group, with a complex and challenging taxonomy due to morphological variations and vast geographical distribution. Although genetic approaches have been used to clarify phylogenetic and taxonomic aspects of Macrobrachium species, cytogenetic information is still very scarce and mostly focused on chromosome number and morphology. Here, we present chromosome data for three species from the Neotropical region, M. carcinus, M. acanthurus, and M. amazonicum, and one species from the Oriental region, M. rosenbergii. Using conventional cytogenetic approaches and chromosome mapping of repetitive DNAs by fluorescence in situ hybridization (FISH), we identified numerical diversification of the diploid set, within and between both zoogeographic regions. These included M. acanthurus and M. amazonicum sharing diploid chromosomes of 98, while M. carcinus has 94, and M. rosenbergii has 118 chromosomes. Argentophilic sites are also variable in number, but they occur in a much higher number than 18S rDNA, representing two to 10 sites within the study species. Microsatellites repeat motifs are also abundant in the chromosomes, with a co-localization and uniform distribution along the chromosome arms, but completely absent in the AT-rich centromeric regions. As a whole, our study suggests that the 2n divergence was followed by a considerable rDNA diversification. The abundance of the exceptional amount of microsatellite sequences in the chromosomes also suggests that they are essential components of the Macrobrachium genome and, therefore, maintained as a shared feature by the species, the reason for which is yet unknown.

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Karyotype, ribosomal genes, and telomeric sequences in the crayfish Procambarus clarkii (Decapoda: Cambaridae)

Cited by 12 publications

References 24 publications

Comparative cytogenetic analysis of marine Palaemon species reveals a X1X1X2X2/X1X2Y sex chromosome system in Palaemon elegans

Comparative cytogenetic analysis of marine Palaemon species reveals a X1X1X2X2/X1X2Y sex chromosome system in Palaemon elegans

Co-localization of ribosomal and telomeric sequences in Leptynia (Insecta: Phasmatodea)

Molecular Cytogenetic Analysis in Freshwater Prawns of the Genus Macrobrachium (Crustacea: Decapoda: Palaemonidae)

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