Karyotype evolution in B‐cell lymphoid malignancy with AN 8;14 translocation

Abstract

To evaluate the significance of karyotypic evolution of tumor cells with an 8;14 translocation [t(8;14)(q24;q32)], we examined the clinicopathologic features and immunologic phenotypes of nine Japanese patients with various types of B-cell malignancy with the translocation. All these patients had structural rearrangements of the long arm of chromosome No. I (Iq) in a stem line or the subline of tumor cells with a t(8;14). The rearrangements were composed of a translocation involving Iq with other chromosomes and a tandem duplication of Iq, and they were exclusively associated with a partial trisomy for Iq. Two patients with diffuse large-cell lymphoma, whose tumor cells did not express surface immunoglobulins (s-Ig), had the Iq translocation in their highly complex karyotypes. Tumor cells from the other seven patients expressed s-Ig and the karyotypes were relatively simple. Among these patients, the Iq translocation was found in two patients with Burkitt's lymphoma, and the Iq duplication were observed in a stem line or the sublines from four patients with Burkitt's lymphoma-leukemia and one each with small non-cleaved-cell or diffuse large-cell lymphoma. Except for one patient in the stage of IE, these patients had a poor prognosis because of the clinical conversion of extranodal metastases in the earlier disease phase. These findings are compatible with those of Western patients with a t(8;14). Therefore, tumor cells marked primarily with a t(8;14) could have "major routes" in the karyotypic evolution, for which potentials should be recognized as clinical risk factors, and the morphologic presentation and the expression of surface immunoglobulins may be associated with the process of karyotypic evolution.

Recurrent chromosome abnormalities in adult T‐cell lymphomas of peripheral T‐cell origin

“…A 14p translocation involving band pll-13 has occasionally been observed in adult T-cell leukemia and childhood Tlymphoblastic leukemia (Miyoshi et al, 1981;Kaneko et al, 1982b;Miyamoto et al, 1984;Hayashi et al, 1985). To our knowledge, only 2 patients with B-cell lymphoma had a translocation involving 14p (Bloomfield et al, 1983;Fukuhara et al, 1983). Therefore, a translocation involving the short arm of chromosome 14 may be one of the most common chromosome abnormalities in lymphoid cancers of T-cell lineage; however, whether the translocation has a consistent break in the region from band 14pll to 1 4~1 3 remains to be determined.…”

“…Spontaneous rosette formation with sheep erythrocytes (E-RFC) was used for evaluation of neoplastic T-cells in all patients. Monoclonal antibodies OKT3, OKT4, OKT6, and OKT8 were also used in the 10 patients for the determination of T-cell differentiation antigens (Nasu et al, 1983). The anti-ATLA(HTLV-I) antibody in serum from 7 patients was titrated by the indirect immunofluorescence method with MT-1 (Hinuma et al, 1981).…”