Karyotype, chromosomal characteristics of multiple rDNA clusters and intragenomic variability of ribosomal ITS2 in Caryophyllaeides fennica (Cestoda)

Orosová, Martina; Králová-Hromadová, Ivica; Bazsalovicsová, Eva; Špakulová, Marta

doi:10.1016/j.parint.2010.04.007

Cited by 28 publications

(19 citation statements)

References 57 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Intragenomic divergent copies were proven to be linked with multiple ribosomal loci Orosová et al 2010), which correlates well with the theory that homogenization processes involved in the evolution of ribosomal genes are less efficient if rDNA is dispersed on multiple loci (van Herwerden et al 1999). In the present paper, however, application of ITS paralogues was used for the first time in this cestode order for interspecific comparison of two congeneric species, which were considered conspecific (Chubb et al 1996), even though without any morphological evidence.…”

Section: Discussionsupporting

confidence: 72%

Molecular characterization of Atractolytocestus sagittatus (Cestoda: Caryophyllidea), monozoic parasite of common carp, and its differentiation from the invasive species Atractolytocestus huronensis

et al. 2011

View full text Add to dashboard Cite

Sequence structure of complete internal transcribed spacer 1 and 2 (ITS1 and ITS2) of the ribosomal DNA region and partial mitochondrial cytochrome c oxidase subunit I (cox1) gene sequences were studied in the monozoic tapeworm Atractolytocestus sagittatus (Kulakovskaya et Akhmerov, 1965) (Cestoda: Caryophyllidea), a parasite of common carp (Cyprinus carpio carpio L.). Intraindividual sequence diversity was observed in both ribosomal spacers. In ITS1, a total number of 19 recombinant clones yielded eight different sequence types (pairwise sequence identity, 99.7-100%) which, however, did not resemble the structure typical for divergent intragenomic ITS copies (paralogues). Polymorphism was displayed by several single nucleotide mutations present exclusively in single clones, but variation in the number of short repetitive motifs was not observed. In ITS2, a total of 21 recombinant clones yielded ten different sequence types (pairwise sequence identity, 97.5-100%). They were mostly characterized by a varying number of (TCGT)(n) repeats resulting in assortment of ITS2 sequences into two sequence variants, which reflected the structure specific for ITS paralogues. The third DNA region analysed, mitochondrial cox1 gene (669 bp) was detected to be 100% identical in all studied A. sagittatus individuals. Comparison of molecular data on A. sagittatus with those on Atractolytocestus huronensis Anthony, 1958, an invasive parasite of common carp, has shown that interspecific differences significantly exceeded intraspecific variation in both ribosomal spacers (81.4-82.5% in ITS1, 74.4-75.2% in ITS2) as well as in mitochondrial cox1, which confirms validity of both congeneric tapeworms parasitic in the same fish host.

show abstract

Section: Discussionsupporting

confidence: 72%

Molecular characterization of Atractolytocestus sagittatus (Cestoda: Caryophyllidea), monozoic parasite of common carp, and its differentiation from the invasive species Atractolytocestus huronensis

et al. 2011

View full text Add to dashboard Cite

show abstract

“…ITS intragenomic variation has also been reported in other species of grasshoppers, such as C. parallelus (Parkin and Butlin, 2004) and Podisma pedestris (Keller et al, 2006), and in other groups of organisms, such as nematodes (Hugall et al, 1999), crabs (Harris and Crandall, 2000), crustaceans (Gandolfi et al, 2001), sponges (Wörheide et al, 2004), mosquitoes (Alquezar et al, 2010), tapeworms (Orosová et al, 2010) and plants (Nieto-Felliner, Roselló, 2007;Song et al, 2012). Such a high intragenomic variation between paralogous copies of rDNA units could lead to highly divergent phylogenetic results, depending on the specific copy or copies sampled.…”

Section: Discussionmentioning

confidence: 80%

Disparate molecular evolution of two types of repetitive DNAs in the genome of the grasshopper Eyprepocnemis plorans

et al. 2013

View full text Add to dashboard Cite

Wide arrays of repetitive DNA sequences form an important part of eukaryotic genomes. These repeats appear to evolve as coherent families, where repeats within a family are more similar to each other than to other orthologous representatives in related species. The continuous homogenization of repeats, through selective and non-selective processes, is termed concerted evolution. Ascertaining the level of variation between repeats is crucial to determining which evolutionary model best explains the homogenization observed for these sequences. Here, for the grasshopper Eyprepocnemis plorans, we present the analysis of intragenomic diversity for two repetitive DNA sequences (a satellite DNA (satDNA) and the 45S rDNA) resulting from the independent microdissection of several chromosomes. Our results show different homogenization patterns for these two kinds of paralogous DNA sequences, with a high between-chromosome structure for rDNA but no structure at all for the satDNA. This difference is puzzling, considering the adjacent localization of the two repetitive DNAs on paracentromeric regions in most chromosomes. The disparate homogenization patterns detected for these two repetitive DNA sequences suggest that several processes participate in the concerted evolution in E. plorans, and that these mechanisms might not work as genome-wide processes but rather as sequence-specific ones.

show abstract

“…In K. sinensis and K. saurogobii, small heterochromatin blocks were observed on all chromosomes, except for the biggest metacentric chromosome pair (present data, Orosová et al 2010a). The presence of heterochromatin blocks out of the pericentromeric region is occasional in Cestoda karyotypes and has been reported only in C. fennica (Orosová et al 2010b). In particular, due to the presence of small positive blocks limited to the centromeric area, it may be assumed that the cestode genome contains a low heterochromatin amount.…”

Section: Discussionmentioning

confidence: 38%

“…It is apparent that chromosomal data could help establish interrelationships among species for taxonomic purposes (Petkevičiūtė 1992). The evident differences between the karyotypes of C. fennica and other karyologically studied lytocestid species do not support the current taxonomic placement of this species into family Lytocestidae (Orosová et al 2010b).…”

Section: Discussionmentioning

confidence: 94%

“…3). The only exception is Caryophyllaeides fennica, having solely metacentric (bi-armed) elements in the complement and moreover different pattern of heterochromatin distribution (discussed below) (Orosová et al 2010b). Recent cladistic investigation revealed that all caryophyllidean families are paraphyletic (Olson et al 2008;Oros et al 2008) and that the present familial classification, based on the position of the inner longitudinal musculature (Mackiewicz 1994), is artificial.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Classical and molecular cytogenetics of Khawia sinensis (Cestoda: Caryophyllidea), invasive parasite of carp, Cyprinus carpio

Orosová

Oros

2011

Parasitol Res

Self Cite

View full text Add to dashboard Cite

Chromosomes of the invasive tapeworm Khawia sinensis (Caryophyllidea), the specific parasite of common carp, were analyzed by means of conventional Giemsa staining and using fluorescent DAPI and YOYO-1 dyes, silver staining, and fluorescent in situ hybridization (FISH) with 18S rDNA probe. The karyotype is composed of eight pairs of metacentric and telocentric chromosomes (2n = 16, n = 3m + 5t, TCL = 42.54 μm). Constitutive heterochromatin was located at pericentromeric regions of all pairs, except for the largest metacentric pair (no. 1), which possessed no DAPI-positive band. FISH with rDNA probe revealed that both homologues of chromosome pair no. 6 carry a cluster of ribosomal arrays, which were located interstitially close to the centromere. Present results are compared with previous cytogenetic data on Khawia spp., and comments are made on the karyotypes with respect to their phylogenetic links.

show abstract

Karyotype, chromosomal characteristics of multiple rDNA clusters and intragenomic variability of ribosomal ITS2 in Caryophyllaeides fennica (Cestoda)

Cited by 28 publications

References 57 publications

Molecular characterization of Atractolytocestus sagittatus (Cestoda: Caryophyllidea), monozoic parasite of common carp, and its differentiation from the invasive species Atractolytocestus huronensis

Molecular characterization of Atractolytocestus sagittatus (Cestoda: Caryophyllidea), monozoic parasite of common carp, and its differentiation from the invasive species Atractolytocestus huronensis

Disparate molecular evolution of two types of repetitive DNAs in the genome of the grasshopper Eyprepocnemis plorans

Classical and molecular cytogenetics of Khawia sinensis (Cestoda: Caryophyllidea), invasive parasite of carp, Cyprinus carpio

Contact Info

Product

Resources

About