Karyomorphology and GC-rich heterochromatin pattern in Passiflora (Passifloraceae) wild species from Decaloba and Passiflora subgenera

Melo, Cláusio Antônio Ferreira de; Souza, Margarete Magalhães; Abreu, Priscilla Patrocínio; Viana, Américo José Carvalho

doi:10.1016/j.flora.2014.08.009

Cited by 18 publications

(22 citation statements)

References 23 publications

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“…The presence of CMA3 + blocks in the terminal region of some chromosomes is generally related to the nucleolar organizing regions (NORs) observed in angiosperms (Guerra, 2000). In species of the genus Passiflora, GC-rich regions detected by fluorochrome CMA3 are observed only in 45S rDNA/satellite DNA sites (Melo et al, 2001;Viana & Souza, 2012;Melo et al, 2014;Belo et al, 2015). The distribution of satellite DNA/45S rDNA regarding CMA3 was analyzed in some species of the genus Citrus, and both probes hybridized only with CMA3 and the centromeres (Silva et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

“…Cytogenetic information has allowed comparison between taxa and the identification of inter-and intraspecific chromosomal variations (Souza et al, 2010;Melo et al, 2011;Guerra, 2013;Melo et al, 2014;Melo et al, 2015;Coelho et al, 2016;Melo et al, 2017). More detailed information on chromosome structure has been developed in recent studies demonstrating the potential of cytogenetic analysis between taxa for comparisons of genetic and cytogenetic maps among different species and genera (Snowdon et al, 1997).…”

Section: Introductionmentioning

confidence: 99%

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Karyotypic characterization of melon accessions

Santos-Sanchês¹,

Souza²,

Melo³

et al. 2019

Científica

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Cucumis melo (melon) is a species from the Iberian Peninsula and is included in the family Cucurbitaceae. Despite the knowledge about the physical structure of melon chromosomes, little is known about the intraspecific karyotype diversity of the species. To study karyotype diversity in eight melon accessions, the following methods were used: Giemsa 3% staining; application of CMA3/DAPI fluorochromes; and location of 45S and 5S rDNA sequences by fluorescence in situ hybridization. Conventional staining analysis revealed stability in the chromosome number, with accessions presenting 2n = 24. There were significant variations in the mean chromosome size between accessions, ranging from 0.98 μm to 1.46 μm for accessions A26 and A18, respectively. Scott-Knott clustering distributed the accessions into two groups. GC-rich heterochromatic blocks (CMA3 + /DAPI-) were observed in pericentromeric regions of all chromosomes in the complement. CMA3 + /DAPIblocks were also located in terminal regions, being specific to satellite regions. Hybridization sites of 45S rDNA probes revealed the presence of a chromosome pair with this locus. In addition, 5S rDNA sites revealed a labeled chromosome pair. No quantitative variation was observed in rDNA sites between the accessions analyzed, indicating these markers as ideal for the verification of karyotypic stability in C. melo.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Karyotypic characterization of melon accessions

Santos-Sanchês¹,

Souza²,

Melo³

et al. 2019

Científica

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“…Giemsa staining was performed according to Melo et al . () with adjustment of staining time. The slides were immersed in a Coplin jar with 3% Giemsa azure eosin methylene blue solution (109204; Merck Millipore, Darmstadt, Germany) for 25 min.…”

Section: Methodsmentioning

confidence: 99%

“…Use of differential staining techniques, such the double‐staining using fluorochromes CMA 3 (chromomycin A3) and DAPI (4′,6‐diamidino‐2‐phenylindole), reveal the heterochromatin quantity and demonstrate that GC‐rich sites are restricted to the nucleolus organisation region (NOR) and satellites in Passiflora (Melo et al . ). For molecular cytogenetics, only hybridisation of ribosomal DNA (rDNA) probes (Melo & Guerra ) for localisation of 5S and 45S rDNA sites has been performed in Passiflora species.…”

Section: Introductionmentioning

confidence: 97%

Cytogenetics and morphological delimitation between three species of Passiflora L. (subgenus Distephana Cervi)

2019

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Several studies on cytogenetic characterisation of passion flowers are helpful to elucidate doubts about taxa relationships, delimitation and classification into more coherent groups based on karyomorphological data.• Molecular and conventional cytogenetic techniques were applied to three Passiflora species with red flowers, P. coccinea, P. vitifolia and P. tholozanii, for species karyotype relationships. Additionally, for descriptive morphology, were used flowers, leaves and seeds.• Results describe for the first time the karyomorphological and chromosome number (2n = 18) for P. tholozanii. ANOVA was performed (P < 0.05) and statistical significance for average chromosome size (CV: 16.53%) between species. Genomic in situ hybridisation (GISH) proved relationships between P. coccinea and P. tholozanii, which suggests a common origin, however, we could not identify hybridisation between genomic probes from P. vitifolia in P. tholozanii chromosomes. Among the species analysed, P. tholozanii has great similarity in karyotypic and morphology to P. coccinea but not to P. vitifolia.• We suggest the inclusion of P. tholozanii in the same subgenus and section as P. coccinea based on the similarity in karyomorphological and morphological traits between the species. Additionally, GISH might indicate a common or hybrid origin of P. tholozanii.Plant Biology 21 (2019) 662-669

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“…The conventional cytogenetic characterization allows the description of the number and morphology of chromosomes and the number and location of satellites, whereas the location and qualitative description of the heterochromatin can be carried out through the use of DAPI and CMA 3 fluorochromes, which characterize the heterochromatin in relation to the richness of AT and GC, respectively (Melo et al 2014). However, the inclusion of more recent techniques that make possible the localization of specific repetitive sequences, such as the fluorescent in situ hybridization (FISH) with 5S and 45S rDNA probes, contribute for the karyotype detailing, being widely used in phylogenetic and evolutive studies, as well as in plant genetic improvement programs (Leitch et al 1994).…”

Section: Introductionmentioning

confidence: 99%

Reproductive and cytogenetic characterization in Passiflora sublanceolata

Belo

Souza

et al. 2015

Biologia

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Reproductive biology (pollen-ovule ratio, pollen viability, germination in vitro pollination and stigma receptivity in vivo) and karyotype characterization by classical and molecular techniques were performed in Passiflora sublanceolata. The pollen-ovule ratio was 83.9, suggesting that this species is facultative autogamous. Pollen viability was below 70% during all anthesis period (6:00 a.m. to 12:00 p.m.). Low in vitro germination rates were observed after anthesis beginning, with none percentage at one, four and six hours and medium percentage (52.9%) at five hour. The stigma remained receptive during the whole anthesis (100%). The average fertilization percentages resulting from controlled pollinations varied between 8.4% at six hour after anthesis beginning and 50% at two and three hour. The fruit characteristics did not show significant differences by the effects of pollination time. The chromosome number was 2n = 22. The average chromosome length and the haploid chromosome length were 1.60 µm and 17.61 µm, respectively. Six CMA + 3 /DAPI − terminal blocks were observed. By means of the FISH technique it was found that the species presented six 45S rDNA terminal sites, two 5S rDNA sites, terminal and pericentromeric, and telomeric hybridization sites associated only to the terminal regions. Passiflora sublanceolata has great reproductive potential and cytogenetic stability as diploid plant. The results obtained in this work will assist in the use of P. sublanceolata as genitor in interspecific hybridizations with other taxa for breeding programs primarily aimed to obtain hybrids for ornamental plant market.

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Karyomorphology and GC-rich heterochromatin pattern in Passiflora (Passifloraceae) wild species from Decaloba and Passiflora subgenera

Cited by 18 publications

References 23 publications

Karyotypic characterization of melon accessions

Karyotypic characterization of melon accessions

Cytogenetics and morphological delimitation between three species of Passiflora L. (subgenus Distephana Cervi)

Reproductive and cytogenetic characterization in Passiflora sublanceolata

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