1994
DOI: 10.2216/i0031-8884-33-1-42.1
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Karyology, nuclear DNA quantification and nucleus-cytoplasmic domain variations in some multinucleate green algae (Siphonocladales, Chlorophyta)

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Cited by 27 publications
(23 citation statements)
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“…The number of nuclei in cladophoralean species is highly variable and generally proportional to cell size. Most cladophoralean taxa have relatively large cells (ranging from several mm to several mm across) with hundreds or even thousands of nuclei, arranged in cytoplasmic domains (Kapraun & Nguyen, 1994). The cells of U. curvatum typically contain 2-4 nuclei (Rueness, 1992;Maggs & O'Kelly, 2007), comparable to numbers found in Rhizoclonium riparium (Roth) Harvey, which has cell dimensions of the same order of magnitude (mostly 5-20 mm in diameter) .…”
Section: Discussionmentioning
confidence: 99%
“…The number of nuclei in cladophoralean species is highly variable and generally proportional to cell size. Most cladophoralean taxa have relatively large cells (ranging from several mm to several mm across) with hundreds or even thousands of nuclei, arranged in cytoplasmic domains (Kapraun & Nguyen, 1994). The cells of U. curvatum typically contain 2-4 nuclei (Rueness, 1992;Maggs & O'Kelly, 2007), comparable to numbers found in Rhizoclonium riparium (Roth) Harvey, which has cell dimensions of the same order of magnitude (mostly 5-20 mm in diameter) .…”
Section: Discussionmentioning
confidence: 99%
“…Extracted erythrocytes were spread evenly on a slide, allowed to air dry, stained with 0.5 lg/mL DAPI for 30 min, and rinsed in phosphate buffered saline (PBS) for 10 min before being cover-slipped. To determine DNA content, fish erythrocyte mean fluorescence intensity values (I f ) were compared with those for DAPI-stained chicken erythrocytes (Kapraun and Nguyen 1994;Kapraun and Dunwoody 2002) with a known DNA content of 2.5 pg (Gregory 2007). DAPI binds by a non-intercalative mechanism to adenine and thymine rich regions of DNA which contain at least four A-T base pairs (Portugal and Waring 1988).…”
Section: Erythrocyte Dna Contentmentioning
confidence: 99%
“…Nuclear DNA content, referred to as C-values (Gregory, 2005), represents multiples of the minimum amount of DNA corresponding to the non-replicated haploid chromosome complement (Gall, 1981;Goff & Coleman, 1984). In this study, the determination of the nuclear ploidy level was based on the positive correlation between DNA content and nuclear size (Price, 1976;Whittick, 1986;Kapraun, 1994;Kapraun & Nguyen, 1994). By measuring the relative fluorescence area (rfa) of DAPI-stained nuclear DNA, the cell-cycle stage and the C-value of the cells were inferred.…”
Section: Nuclear Dna Analysismentioning
confidence: 99%
“…Microspectrophotometry has been widely used to demonstrate nuclear DNA content variation associated with haploid and diploid phases (Goff & Coleman, 1984;Deshmukhe & Tatewaki, 1993;Bennett & Leitch, 2005;Kapraun, 2007), but the required equipment is expensive and increasingly unavailable (Choi et al, 1994). In contrast, image analysis systems which provide accurate relative nuclear DNA content estimates (Vilhar et al, 2001;Hardie et al, 2002) are more readily available and have been successfully used for algal investigations (Choi et al, 1994;Kapraun & Nguyen, 1994;Lee et al, 1995;Masuda et al, 1996;Schnetter et al, 2000;Bleckwenn et al, 2003).…”
Section: Introductionmentioning
confidence: 99%