Karyological studies on established mosquito cell lines

Chiplonkar, J. M.; Wagh, U. V.; Phondke, G P; Joshi, Deeksha

doi:10.1007/bf01971121

Cited by 4 publications

(4 citation statements)

References 6 publications

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“…The C6/36 subclone was selected for its uniformly high virus yield and was shown to retain a diploid karyotype with 2n = 6 chromosomes in a majority of cells [ 4 ]. The similar or equivalent ATC-15 cells [ 1 ] were shown to be diploid [ 5 ] and to have more chromosomal abnormalities after 110 passages than after 17 [ 6 ]. The C6/36 cell line, available through the American Type Culture Collection (ATCC; Manassas, VA, USA), is described as maintaining a diploid chromosome number and being non-anchorage-dependent and nontumorigenic [ 7 ].…”

Section: Introductionmentioning

confidence: 99%

Analysis of the Aedes albopictus C6/36 genome provides insight into cell line utility for viral propagation

et al. 2018

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BackgroundThe 50-year-old Aedes albopictus C6/36 cell line is a resource for the detection, amplification, and analysis of mosquito-borne viruses including Zika, dengue, and chikungunya. The cell line is derived from an unknown number of larvae from an unspecified strain of Aedes albopictus mosquitoes. Toward improved utility of the cell line for research in virus transmission, we present an annotated assembly of the C6/36 genome.ResultsThe C6/36 genome assembly has the largest contig N50 (3.3 Mbp) of any mosquito assembly, presents the sequences of both haplotypes for most of the diploid genome, reveals independent null mutations in both alleles of the Dicer locus, and indicates a male-specific genome. Gene annotation was computed with publicly available mosquito transcript sequences. Gene expression data from cell line RNA sequence identified enrichment of growth-related pathways and conspicuous deficiency in aquaporins and inward rectifier K+ channels. As a test of utility, RNA sequence data from Zika-infected cells were mapped to the C6/36 genome and transcriptome assemblies. Host subtraction reduced the data set by 89%, enabling faster characterization of nonhost reads.ConclusionsThe C6/36 genome sequence and annotation should enable additional uses of the cell line to study arbovirus vector interactions and interventions aimed at restricting the spread of human disease.

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Section: Introductionmentioning

confidence: 99%

Analysis of the Aedes albopictus C6/36 genome provides insight into cell line utility for viral propagation

et al. 2018

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“…Further studies are required to explore this potentially interesting phenomenon. Although rare (around 1% of insect cell lines, [8]), mosquito and Drosophila cell lines with at least partially haploid karyotypes have been reported previously [32][33][34].…”

Section: Discussionmentioning

confidence: 99%

Establishment and partial characterisation of a new cell line derived from adult tissues of the tsetse fly Glossina morsitans morsitans

Bell-Sakyi,

Haines,

Petrucci

et al. 2024

Parasites Vectors

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Background Insect cell lines play a vital role in many aspects of research on disease vectors and agricultural pests. The tsetse fly Glossina morsitans morsitans is an important vector of salivarian trypanosomes in sub-Saharan Africa and, as such, is a major constraint on human health and agricultural development in the region. Methods Here, we report establishment and partial characterisation of a cell line, GMA/LULS61, derived from tissues of adult female G. m. morsitans. GMA/LULS61 cells, grown at 28 °C in L-15 (Leibovitz) medium supplemented with foetal bovine serum and tryptose phosphate broth, have been taken through 23 passages to date and can be split 1:1 at 2-week intervals. Karyotyping at passage 17 revealed a predominantly haploid chromosome complement. Species origin and absence of contaminating bacteria were confirmed by PCR amplification and sequencing of fragments of the COI gene and pan-bacterial 16S rRNA gene respectively. However, PCR screening of RNA extracted from GMA/LULS61 cells confirmed presence of the recently described Glossina morsitans morsitans iflavirus and Glossina morsitans morsitans negevirus, but absence of Glossina pallipides salivary gland hypertrophy virus. GMA/LULS61 cells supported infection and growth of 6/7 different insect-derived strains of the intracellular bacterial symbiont Wolbachia. Conclusions The GMA/LULS61 cell line has potential for application in a variety of studies investigating the biology of G. m. morsitans and its associated pathogenic and symbiotic microorganisms. Graphical Abstract

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“…2). The wide distribution of DNA values per cell in these lines indicate the inherent heterogeneity (6).…”

Section: Flow Cytometrymentioning

confidence: 93%

“…Primary cultures of Aedes albop ictus larvae were set as described by Singh (19). CHROMOSOME ANALYSIS Chromosome studies were made on colchicin-arrested, giemsa-stained metaphase spreads of ATC-10, ATC-15, ATC-136, and ATC-173 cell lines at late passages (6). These were analysed for frequency distribution and structural abnormalities and peculiarities.…”

mentioning

confidence: 99%

Studies on characterization of mosquito cell lines

et al. 1984

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Results of studies on characterization of mosquito cell lines are described; these include chromosome analysis, image cytometric, and flow cytometric estimation of DNA, tumorogenicity and angiogenicity, concanavalin‐A‐induced agglutination, and proton spin‐lattice relaxation time (T1). The established mosquito cell lines are not diploid lines, although their stem‐lines are diploid. Feulgen cytometry and flow cytometry reveal the inherent heterogeneity in DNA contents and support the observations on chromosome frequency distribution. Two cell lines exhibit angiogenic property and cell infiltration inchorio‐allantoic membrane of chick embryos; however, none of the cell lines tested by inoculating hamsters, mice, or adult mosquitoes exhibit tumorogenicity. The agglutinability patterns of these cells in response to concanavalin‐A treatment reveal the coexistence of agglutinable and unagglutinable cells. The mosquito cells differ from mammalian and chick cells in respect of their proton spinlattice relaxation time.

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Karyological studies on established mosquito cell lines

Cited by 4 publications

References 6 publications

Analysis of the Aedes albopictus C6/36 genome provides insight into cell line utility for viral propagation

Analysis of the Aedes albopictus C6/36 genome provides insight into cell line utility for viral propagation

Establishment and partial characterisation of a new cell line derived from adult tissues of the tsetse fly Glossina morsitans morsitans

Studies on characterization of mosquito cell lines

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