Studies on characterization of mosquito cell lines

Abstract

Results of studies on characterization of mosquito cell lines are described; these include chromosome analysis, image cytometric, and flow cytometric estimation of DNA, tumorogenicity and angiogenicity, concanavalin‐A‐induced agglutination, and proton spin‐lattice relaxation time (T1). The established mosquito cell lines are not diploid lines, although their stem‐lines are diploid. Feulgen cytometry and flow cytometry reveal the inherent heterogeneity in DNA contents and support the observations on chromosome frequency distribution. Two cell lines exhibit angiogenic property and cell infiltration inchorio‐allantoic membrane of chick embryos; however, none of the cell lines tested by inoculating hamsters, mice, or adult mosquitoes exhibit tumorogenicity. The agglutinability patterns of these cells in response to concanavalin‐A treatment reveal the coexistence of agglutinable and unagglutinable cells. The mosquito cells differ from mammalian and chick cells in respect of their proton spinlattice relaxation time.

Analysis of the Aedes albopictus C6/36 genome provides insight into cell line utility for viral propagation

“…The C6/36 subclone was selected for its uniformly high virus yield and was shown to retain a diploid karyotype with 2n = 6 chromosomes in a majority of cells [ 4 ]. The similar or equivalent ATC-15 cells [ 1 ] were shown to be diploid [ 5 ] and to have more chromosomal abnormalities after 110 passages than after 17 [ 6 ]. The C6/36 cell line, available through the American Type Culture Collection (ATCC; Manassas, VA, USA), is described as maintaining a diploid chromosome number and being non-anchorage-dependent and nontumorigenic [ 7 ].…”