Karyological studies in twelve accessions ofCarthamus tinctoriusi

Abstract

Karyology of the twelve accessions of Carthamus tinctorius L. were investigated. Diploid chromosome numbers and the basic chromosome number of the studied accessions were obtained as 2n=24 and x=12 respectively. The chromosomes were assorted in to different categories on the basis of arm's ratio. These were further subdivided in to different types, on the basis of total length of the chromosome. From an evolutionary point of view, variation in total chromosome length without major changes in the karyotype formula suggests that changes in the amounts of genomic DNA are proportional to the relative length of each chromosome arm. All the accessions possessed symmetrical or slightly asymmetrical karyotype. The karyological results of the study and the analysis of chromosomal morphology of each accession are shown as tables. These findings may provide useful information for Carthamus evolutionary, genetic, and breeding studies.

Karyotype analysis of some lines and varieties belonging to Carthamus tinctorius L. species

“…All of the studied varieties and lines have a chromosome number of 2X=2n=24 as supported with the earlier studies (Knowles 1988, Anjali and Srivastava, 2012, Yazdani 2013). On the whole, karyotypes of the most analysed varieties had a predominance of metacentric (mc) chromosomes.…”

“…Tcar 13 (E12) had larger chromosomes as well as a larger genome than other samples. Anjali and Srivastava (2012) investigated karyomorphological features of twelve accessions of C. tinctorius. Their total chromosome lengths range from 38.79 -55.56 µm.…”

“…Carthamus L. genus consists of about 25 main species in the world. Carthamus tinctorius, commonly called safflower, is the only cultivated species of this genus (Anjali and Srivastava, 2012). Safflower, C. tinctorius L., is a member of the family Compositae or Asteraceae, cultivated mainly for its seed, which is used as edible oil and as birdseed.…”

Karyotype Analysis Using C- and NOR-Banding in Ramie [<i>Boehmeria nivea</i> (L.) Gaud.]

“…The karyomorphometric data along with the different indices for estimating the karyotype asymmetry have been recognized and employed as part of the prebreeding stage in several crops. Moreover, chromosome characterization has also allowed for the detection of chromosome polymorphism in Chinese populations of Salvia miltiorrhiza (2n = 18) (Song et al 2010b), the identification of a novel germplasm and the diversity in different accessions of Carthamus tinctorius (Anjali and Srivastava 2012), and the distinction of three hybrids in ornamental hydrangeas (hortensias) (Van Laere et al 2008), for instance. The use of karyotypes in comparative studies has been a good parameter for changing estimates in the genome, which is frequently related to evolution in higher plants (Romero Zarco 1986).…”

Analisis Kromosom Varietas Kacang Gude (Cajanus cajan (L.) Millspaugh) Asal Pulau Kisar, Maluku Barat Daya (MBD)

“…Tujuan analisis kromosom adalah mempermudah mempelajari identitas kromosom. Anjali & Srivastava (2012) menambahkan bahwa analisis kromosom, misalnya kariotipe mempunyai manfaat menyampaikan informasi tentang identifikasi spesies dan analisis hibrida. Zayed & Zeinab (2016) menjelaskan bahwa jumlah, ukuran dan bentuk kromosom merupakan komponen dalam mengkarakterisasi kariotipe dan mendefinisikan perbedaan taksonomi.…”