Kartogenin Induced Adipose-Derived Stem Cell Exosomes Enhance the Chondrogenic Differentiation Ability of Adipose-Derived Stem Cells

Xie, Aiguo; Xue, Jixin; Wang, Yuting; Yang, Chao; Xu, Miao; Jiang, Yongkang

doi:10.1155/2022/6943630

Cited by 3 publications

(2 citation statements)

References 38 publications

(46 reference statements)

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“…Adipose derived stem cell (ADSC), the largest source of easily isolatable adult stem cells, are widely used for regenerative medicine and tissue engineering as they can self-renew and differentiate into various cell types, such as osteoblasts, , adipocytes, , chondrocytes, , and smooth muscle cells. , A major challenge in this field is guiding their differentiation into specific cell types and maintaining their specialized characteristics in synthetic 3D environments, such as model tissue scaffolds. To address this issue, we have used fiber loaded gels to evaluate the differentiation potential of ADSC into osteogenic and adipogenic linage.…”

Section: Resultsmentioning

confidence: 99%

Magnetic Nanofibrous Hydrogels for Dynamic Control of Stem Cell Differentiation

Islam,

Molley,

Hung

et al. 2023

ACS Appl. Mater. Interfaces

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The extracellular matrix in tissue consists of complex heterogeneous soft materials with hierarchical structure and dynamic mechanical properties dictating cell and tissue level function. In many natural matrices, there are nanofibrous structures that serve to guide cell activity and dictate the form and function of tissue. Synthetic hydrogels with integrated nanofibers can mimic the structural properties of native tissue; however, model systems with dynamic mechanical properties remain elusive. Here we demonstrate modular nanofibrous hydrogels that can be reversibly stiffened in response to applied magnetic fields. Iron oxide nanoparticles were incorporated into gelatin nanofibers through electrospinning, followed by chemical stabilization and fragmentation. These magnetoactive nanofibers can be mixed with virtually any hydrogel material and reversibly stiffen the matrix at a low fiber content (≤3%). In contrast to previous work, where a large quantity of magnetic material disallowed cell encapsulation, the low nanofiber content allows matrix stiffening with cells in 3D. Using adipose derived stem cells, we show how nanofibrous matrices are beneficial for both osteogenesis and adipogenesis, where stiffening the hydrogel with applied magnetic fields enhances osteogenesis while discouraging adipogenesis. Skeletal myoblast progenitors were used as a model of tissue morphogenesis with matrix stiffening augmenting myogenesis and multinucleated myotube formation. The ability to reversibly stiffen fibrous hydrogels through magnetic stimulation provides a useful tool for studying nanotopography and dynamic mechanics in cell culture, with a scope for stimuli responsive materials for tissue engineering.

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Section: Resultsmentioning

confidence: 99%

Magnetic Nanofibrous Hydrogels for Dynamic Control of Stem Cell Differentiation

Islam,

Molley,

Hung

et al. 2023

ACS Appl. Mater. Interfaces

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“…The hADSCs were induced in adipogenic induction medium for 3 weeks. Alcian blue staining was implemented using the standard Alcian blue kit (pH = 2.5) (Solarbio, Beijing, China) as per the manuals of manufacturer to evaluate chondrogenic capacity, followed by observation of the staining results using an inverted microscope (Olympus) 31 …”

Section: Methodsmentioning

confidence: 99%

Effects of extracellular vesicles from adipose‐derived stem cells on human keloid fibroblasts via the SOCS1/JAK2/STAT3 pathway

Ruan,

Wang,

Shi

et al. 2024

J of Cosmetic Dermatology

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BackgroundKeloid represents a benign skin tumor with many cancer‐like features. Extracellular vesicles (EVs) derived from human adipose‐derived stem cells (hADSCs) play a role in cell migration of multiple diseases.AimsThis study aimed to investigate the impact of hADSC‐EVs on human keloid fibroblasts (HKFs).MethodshADSCs were cultured to the 3rd generation, and subsequently assessed for their osteogenic, adipogenic, and chondrogenic differentiative abilities using flow cytometry, alizarin red, oil red O, and alcian blue staining techniques. hADSC‐EVs were isolated through ultracentrifugation and subsequently identified. HKFs at the 3rd generation were subjected to treatment with hADSC‐EVs to observe their endocytosis of EVs by immunofluorescence. CCK‐8, wound healing, and Transwell assays were performed to test HKF proliferation and migration. The levels of autophagy proteins, collagens, and Janus kinase 2 (JAK2) and Signal Transducer and Activator of Transcription 3 (STAT3) were determined through Western blot analysis. Suppressor of cytokine signaling 1 (SOCS1) expression was determined by RT‐qPCR and Western blot.ResultshADSC‐EVs were successfully isolated from hADSCs. PKH67‐labeled hADSC‐EVs were observed to be endocytosed by HKFs, resulting the inhibition of HKF proliferation, migration, as well as a reduction in collagen deposition. hADSC‐EVs carried SOCS1 into HKFs to suppress HKF autophagy. SOCS1 downregulation in hADSC‐EVs partially nullified the inhibitory effect of hADSC‐EVs on HKFs. hADSC‐EV‐carried SOCS1 inhibited the activation of the JAK2/STAT3 pathway. JAK2/STAT3 pathway activation partially abrogated the suppression of hADSC‐EVs on the proliferation, migration, and collagen deposition of HKF.ConclusionhADSC‐EVs carried SOCS1 into HKFs and suppressed HKF autophagy, proliferation, migration, and collagen deposition by inactivating the JAK2/STAT3 pathway.

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