Some species in the genus of Acacia were introduced in tropical regions mostly for supplying raw material for forest industries, such as pulp and paper, sawn timber and wood energy. Indonesia, Malaysia, Vietnam, China, India, Philipines, and Thailand were reported as the countries concern for Acacia plantations in the tropic areas [1]. The establishment of Acacias plantations were practiced either by large scale plantation companies or by smallholder grower. The two largest species of Acacias for plantation in Indonesia are Acacia mangium and Acacia auriculiformis. Although these two Acacias were found growing naturally in Eastern part of Indonesia [2], the Acacia plantation was firstly introduced in 1975's as a part of national land rehabilitation program [3]. Recently market-oriented has driven the Acacias plantation to be economically feasible for diverse end-product. Current new achievement in wood processing technology have made the Acacias could be potentially improved for varies end-product purposes [4, 5, 6, 7, 8, 9]. In addition, progress of breeding in tree improvement program succeeded to improve the stand productivity of the Acacias and produced new variety of Acacia hybrid, an out crossing between A. mangium and A. auriculiformis [10, 11, 12, 13, 14,15]. Based on these two achievement technologies, recently the chance for the end-product diversification from the Acacias wood becomes more attractive. The knowledge of wood anatomical structure is a fundamental component to support the utilization of wood from a forest tree species. Based on this anatomical structure the uses of wood for suitable endproduct could be explored. However, the anatomical structure of wood may potentially change during the process of breeding either due to the process of selection or gene recombination through a mating [16, 17, 18]. Therefore, this study was conducted to observe the wood anatomical structure of four improved Acacias: A. mangium, A. auriculiformis and the two Acacia hybrids which were resulted from tree improvement program in Indonesia.