Karakteristik Segmen Gen sitokrom C Oksidase Subunit I (COI) Ngengat Plusia chalcites (Lepidoptera: Noctuidae)

Suriana, Suriana; Marwansyah, Marwansyah; Amirullah, Amirullah

doi:10.33772/biowallacea.v6i2.8824

Cited by 7 publications

(9 citation statements)

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“…This effectiveness is caused by low intraspecific variations, especially in adjacent taxa (Hajibabaei et al 2006). It has been established that the COI techniques in other animals, such as metazoan invertebrates (Folmer et al 1994), Tegal ducks (Anas domesticus) (Wibowo et al 2013), Holothuroidea (Sahriyani et al 2014), slow loris (Nycticebus spp) (Wirdateti et al 2016), and Lepidoptera noctuidae (Suriana et al 2019).…”

Section: Introductionmentioning

confidence: 99%

Molecular analysis of Taro and Bali cattle using cytochrome oxidase subunit I (COI) in Indonesia

2020

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Abstract. Susari NNW, Suastika P, Agustina KK. 2021. Molecular analysis of Taro and Bali cattle using cytochrome oxidase subunit I (COI) in Indonesia. Biodiversitas 22: 165-172. Cytochrome oxidase subunit I (COI) is one of the molecular markers often used as a differentiator with many advantages in phylogeny analysis, and it thus rarely undergoes substitution. This research was conducted to characterize the genetics of Taro and Bali (Bos javanicus) cattle. Blood from the animals was collected from the jugularis vein and amplified by PCR. The target area was COI with a primer that was successfully amplified, namely the forward BICOIF (5'-TTC-TCAACCAACCATAAAGATATTGG-3') and the reverse BICOIR fragment (5'-TAG-ACTTCGGGGTGTCCAAAGAATCA-3'). The PCR products' sequencing was carried out by phylogeny analysis using MEGA 6 software. The amplicon value that succeeded in electrophoresis was 710bp, while six polymorphic sites were obtained at base positions of 1, 300, 379, 675, 676, and 679. The haplotypes (Hap) obtained were 4, with a genetic distance that ranged from 0.000-0.001. The nitrogenous bases of the amino acid composition from the samples showed no significant difference. The phylogenetic tree (Tamura-Nei) classified the cattle into two clades with a genetic distance of 0.0005.

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Section: Introductionmentioning

confidence: 99%

Molecular analysis of Taro and Bali cattle using cytochrome oxidase subunit I (COI) in Indonesia

2020

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“…Gen COI (Cytochrome Oxidase Subunit I) terdapat di dalam genom mitokondria. Salah satu gen ini umum dipakai dalam DNA barcoding (Wirdateti et al, 2016) yang berperan penting dalam produksi energi serta memiliki urutan pasang basa bersifat conserve (Suriana et al, 2019).…”

Section: Pendahuluanunclassified

“…Analisis jarak genetik dilakukan dengan melihat tingkat perbedaan gen (perbedaan sekuen DNA) pada suatu populasi atau spesies yang diukur melalui p- ISSN 2550-1232https://doi.org/10.46252/jsai-fpik-unipa.2021.Vol.5.No.1.125 e-ISSN 2550 ©Jurnal Sumberdaya Akuatik Indopasifik, Vol. kuantitas numeric (Saitou & Nei, 1987;Suriana et al, 2019). Jarak genetik yang terbentuk merupakan akibat dari perbedaan genetik spesies dan juga akibat dari respon genetik terhadap kondisi ekologi suatu populasi (Fatmarischa et al, 2014).…”

Section: Jarak Genetik Dan Filogenetikunclassified

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DNA barcoding approach to identification of Cassidula angulifera (Petit, 1841) (Mollusca: Gastropoda)

Saleky¹,

Merly²

2021

JSAI

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A large number of gastropod species have similarities in morphology (cryptic) makes misidentification probably happen/occurred. Accurate species identification is needed in studying bioecology of species. This research aims to identify the species of Cassidulla sp. Which was collected from Peyum Beach Merauke with DNA barcoding techniques using COI gene markers. The primers used in this study are forward primers (LCO1490) and reverse primers (HCO2198). The result of identification with DNA barcoding showed that the species analyzed was Cassidula angulifera with a 99.53% similarity level with a DNA sequence length of 650 bp. Phylogenetic reconstruction showing the entire sequence of Cassidula sp. which were analyzed separately based on the type and genetic distance with high bootstrap value. Phylogenetic reconstruction of Cassidula sp. form a monophyletic group, which means that the species come from the same ancestors. DNA barcoding is very good and accurate in identifying species.

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“…Jarak genetik genetik kurang dari 2 % dapat dikatakan merupakan spesies yang sama (Cai et al, 2016;Ward, 2009 Hasil analisis jarak genetik (Tabel 1) sangat berpengaruh terhadap pohon filonetik yang terbentuk. Hubungan jarak genetik berkaitan dengan rekonstruksi pohon filogenetik (Suriana et al, 2019).…”

Section: Jarak Genetik Dan Filogenetikunclassified

Molecular Phylogenetic of Cerithidea anticipata (Iredale, 1929) (Mollusk: Gastropod)

Saleky

Merly

2021

JIP

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Species identification is very important and an important part of bioecological studies, so phylogenetic studies of Cerithidea anticipata (Iredale, 1929) was conducted in September 2020 to identify C. anticipata (Iredale, 1929) based on DNA barcoding techniques. Samples of C. anticipata (Iredale, 1929) were collected from the mangrove ecosystem of Payum Merauke Beach Papua (Indonesia), where the genes used were primary COI Gene forward LCO1490 and reverse HCO2198. The result of DNA amplification obtained DNA sequence length of 660 bp, then based on the identification of Basic Local Alignment Search Tools (BLAST) obtained a similarity level of 98.42% and phylogenetic reconstruction showed the existence of grouping based on the degree of similarity and genetic distance between populations.Keywords: Cerithidea anticipata; COI genes; DNA barcoding; phylogeneticsAbstrakIdentifikasi spesies sangat penting dilakukan dan menjadi bagian penting dalam studi bioekologi, sehingga kajian filogenetik Cerithidea anticipata (Iredale, 1929) telah dilakukan pada bulan September 2020 dengan tujuan untuk mengidentifikasi C. anticipata (Iredale, 1929) berdasarkan teknik DNA barcoding. Sampel C. anticipata (Iredale, 1929) dikoleksi dari ekosistem mangrove Pantai Payum Merauke Papua (Indonesia), dimana gen yang digunakan adalah Gen COI primer forward LCO1490 dan reverse HCO2198. Hasil dari amplifikasi DNA diperoleh panjang sekuen DNA 660 bp, kemudian berdasarkan identifikasi Basic Local Alignment Search Tools (BLAST) diperoleh tingkat kemiripannya 98.42% dan rekonstruksi filogenetiknya memperlihatkan adanya pengelompokan berdasarkan tingkat kemiripan maupun jarak genetik antar populasi.Kata kunci: Cerithidea anticipata; Gen COI; DNA barcoding; filogenetik

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Karakteristik Segmen Gen sitokrom C Oksidase Subunit I (COI) Ngengat Plusia chalcites (Lepidoptera: Noctuidae)

Cited by 7 publications

References 0 publications

Molecular analysis of Taro and Bali cattle using cytochrome oxidase subunit I (COI) in Indonesia

Molecular analysis of Taro and Bali cattle using cytochrome oxidase subunit I (COI) in Indonesia

DNA barcoding approach to identification of Cassidula angulifera (Petit, 1841) (Mollusca: Gastropoda)

Molecular Phylogenetic of Cerithidea anticipata (Iredale, 1929) (Mollusk: Gastropod)

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