Karakterisasi Gen KIF12 (Kinesin Familly 12) serta Hubungannya dengan Komposisi Asam Lemak pada Domba

Abstract

ABSTRAKKinesin Family 12 (KIF12) merupakan gen yang terlibat dalam mediasi kaskade antioksidan dalam sel beta sebagai sebuah target intraselular dari kelebihan asupan lemak atau lipotoksit. Penelitian ini bertujuan untuk mengidentifikasi karakterisasai gen KIF12 melalui identifikasi keragaman dan asosiasi gen KIF12 terhadap komposisi asam lemak pada domba. Sampel domba yang digunakan sebanyak 35 ekor yang terdiri dari Domba ekor Gemuk (DEG) dan Domba Ekor Tipis (DET) masing-masing 20 dan 15 ekor. Titik mutasi gen KIF12 berada pada posisi g.9617965 C>T. Identifikasi keragaman gen KIF12 dianalisis menggunakan Polymerase Chain Reaction - Restriction Fragment Length Polymorphism (PCR-RFLP) dengan menggunakan enzim BfaI. Hasil keragaman gen KIF12 bersifat polimorfik dengan ditemukan tiga genotipe yaitu CC, CT, dan TT. Hasil Uji chi square menunjukkan bahwa lokus KIF12 berada pada keseimbangan Hardy-Weinberg (HWE) pada pada DET, hal berbeda ditunjukakan pada DEG. Titik mutasi gen KIF12 berasosiasi secara signifikan (P <0.05) terhadap asam lemak jenuh (SFA) asam laurik (C12:0), asam miristik (C14: 0) and asam heptadenoik (C17: 0) juga asam lemak jenuh tunggal (MUFA), asam miristoleinik (C14: 1) and asam oleik (C18: 1n9c). Gen KIF12 memiliki potensi untuk digunakan sebagai penanda seleksi terhadap komposisi asam lemak.Kata kunci: domba, asam lemak, KIF12, PCR RFLPABSTRACTKinesin Family 12 (KIF12) is one of gene which involved in mediates an antioxidant cascade in beta cells as an intracellular target of excess fat intake or lipotoxicitys. This study was aimed to identify the polymorphism and association of KIF12 gene as candidate gene for fatty acid composition in Indonesian sheep including fat-tailed sheep and thin-tailed sheep. The sample of sheeps used 35 heads consist fat-tailed sheep and thin-tailed sheep as many as each 20 and 15 samples. Identification of polymorphism of KIF12|BfaI gene were analyzed using Polymerase Chain Reaction - Restriction Fragment Length Polymorphism (PCR-RFLP). The results of polymorphism of KIF12 gene were polymorphic for three kind of genotypes of CC, CT, and TT. The chi square revealed that the locus of KIF12 was deviated in Hardy-Weinberg equilibrium (HWE) in fat-tailed sheep, but was in HWE in thin-tailed sheep. A SNP of KIF12 was associated (P <0.05) with saturated fatty acids (SFA) including lauric acid (C12:0), myristic acid (C14: 0) and heptadecanoic acid (C17: 0) as well as monounsaturated fatty acids (MUFA), namely miristoleinic acid (C14: 1) and oleic acid (C18: 1n9c).The KIF12 gene was the potential to be used as a marker of selection for fatty acid compositions. Keywords : sheep, fatty acids, KIF12, PCR-RFLP

Polymorphisms KIF12 GENE|BfaI and its Association on Carcass Characteristics and Lamb Meat Quality

“…Kinesin has a relationship with the control of triglyceride secretion from the liver (Rai et al, 2017). Genetic diversity in the KIF12 gene is reported to be associated with the quality of the fatness of lamb meat (Gunawan et al, 2018). Gunawan et al (2018) reported that the Single Nucleotide Polymorphism (SNP) gene KIF12 in lamb was found at position g.9617965 C>T and had a significant association with monounsaturated fatty acids, including myristolenic acid (C14:1), oleic acid (C18:1n9c), and fatty acids.…”

Association of Cytochrome P450 2A6 (CYP2A6) Gene Polymorphisms with Fatty Acid Traits in Indonesian Native Sheep

“…The price of sheep which is relatively more expensive than other animal protein sources is one of the factors contributing to the low consumption of Indonesia. Gunawan et al (2018a) reported that, apart from price, there is an assumption in Indonesian society that lamb meat contains cholesterol and high saturated fatty acid (SFA) content, the odor of meat which is less desirable and difficult to remove are factors that are thought to cause low national consumption of lamb meat. Consumption of SFA, and cholesterol correlates with an increase low density lipoprotein (LDL) in blood.…”

“…A genomic selection-based on breeding program to produce lamb meat with high unsaturated fatty acid and low SFA content are potential to be implemented. Fatty acids composition is a quantitative trait that influenced by genetic factors and controlled by more than one gene (Gunawan et al, 2018a). Fatty acids trait in sheep has a heritability value ranging from 0.25-0.46 which indicates that increase in genetic quality through an effective selection program to be carried out (Rovadoscki et al, 2018).…”

“…Several genes were reported to be significantly associated with fatty acid content in sheep including APOA5 (Gunawan et al, 2018b), AHSG (Munyaneza et al, 2019a), BHMT (Munyaneza et al, 2019b), DGAT1 (Gunawan et al, 2019), and KIF12 (Gunawan et al, 2018a). Cytochrome P450 2A6 (CYP2A6) are genes thought to contribute in the fatty acid traits in sheep.…”