Kalanchosides A—C, New Cytotoxic Bufadienolides from the Aerial Parts of Kalanchoe gracilis.

Wu, Pei‐Lin; Hsu, Yu-Lin; Wu, Tong; Bastow, Kenneth F.; Lee, Kuo‐Hsiung

doi:10.1002/chin.200710204

Cited by 12 publications

(14 citation statements)

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“…In previous phytochemical investigations, methanolic extract of K. gracilis has shown cytotoxic activities against human gastric and nasopharyngeal carcinoma cell lines. In addition, one of the compounds isolated from K. gracilis, bryophyllin, has been shown to inhibit HIV replication in H9 lymphocyte cells (Wu et al, 2006). Since K. gracilis root is rich in polyphenolic compounds, it may be developed into an antioxidant, anti-inflammatory or anticancer agent in the future.…”

Section: Cell Viabilitymentioning

confidence: 99%

“…In Taiwan, it is a folk medicine commonly used by traditional Chinese medicine (TCM) practitioners for the treatment of pain, fever, inflammation and injuries. Previous phytochemical investigations have isolated one coumarin and eight bufadienolides (Wu et al, 2006), as well as nine flavonoids that were further identified as luteolin, quercetin, quercitrin, kaempferol, eupafolin and four glycosidic derivatives of eupafolin (Karin et al, 1989). In our previous study, we demonstrated that K. gracilis increased SOD and GRx activities; its methanol extract was also shown to have analgesic and anti-inflammatory effects in mice (Lai et al, 2010b).…”

Section: Introductionmentioning

confidence: 99%

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Antioxidant, Anti-inflammatory and Antiproliferative Activities of Kalanchoe gracilis (L.) DC Stem

Lai

Huang

et al. 2011

Am. J. Chin. Med.

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Oxidative stress and inflammation are related to several chronic diseases including cancer and atherosclerosis. Kalanchoe gracilis (L.) DC is a special folk medicinal plant in Taiwan. The aim of this study was to evaluate the antioxidant, anti-inflammatory and antiproliferative activities of the methanolic extract and fractions of the stem of K. gracilis. TEAC, total phenolic compound content, total flavonoid content, DPPH radical scavenging activity, reducing power, inhibition of NO production in LPS-induced RAW264.7 cells, and inhibition of cancer cell proliferation were analyzed. Among all fractions, the chloroform fraction showed the highest TEAC and DPPH radical scavenging activities. The chloroform fraction also had the highest content of polyphenols and flavonoids. Chloroform fractions also decreased LPS-induced NO production and expressions of iNOS and COX-2 in RAW264.7 cells. The antiproliferative activities of the methanolic extract and fractions were studied in vitro using HepG2 cells, and the results were consistent with their antioxidant capacities. Chloroform fractions had the highest antiproliferative activity with an IC(50) of 136.85 ± 2.32 μg/ml. Eupafolin also had good pharmacological activity in the antioxidant, anti-inflammation and antiproliferative. Eupafolin might be an important bioactive compound in the stem of K. gracilis. The above experimental data indicated that the stem of K. gracilis is a potent antioxidant medicinal plant, and such efficacy may be mainly attributed to its polyphenolic compounds.

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Section: Cell Viabilitymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Antioxidant, Anti-inflammatory and Antiproliferative Activities of Kalanchoe gracilis (L.) DC Stem

Lai

Huang

et al. 2011

Am. J. Chin. Med.

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“…This activity is commonly described as a 'digitalis-like' effect (Schoner & Scheiner-Bobis 2007). It was established that aerial parts of K. gracilis contains eight bufadienolides, including kalanchosides A, B and C, thesiuside, hellebrigenin, hellebrigenin-3-acetate as well as bryophyllins A and B (Wu et al 2006). In K. daigremontiana Â tubiflora, seven bufadienolides such as bryophyllin A, bryophyllin C, bersaldegenin-3-acetate, bersaldegenin-1,3,5-orthoacetate, daigremontianin, bersaldegenin-1-acetate and methyl daigremonate were found (Supratman et al 2001a,b).…”

Section: Discussionmentioning

confidence: 99%

Antioxidant efficacy of Kalanchoe daigremontiana bufadienolide-rich fraction in blood plasma in vitro

Kołodziejczyk-Czepas

Nowak

Wachowicz

et al. 2016

Pharmaceutical Biology

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Context: The main source of bufadienolides is toad venom; however, plants such as members of Kalanchoe Adans. (Crassulaceae) genus may also synthesize these bioactive substances. Objective: This is the first study on antioxidant effects and cytotoxicity of bufadienolide-rich fraction isolated from Kalanchoe daigremontiana Raym.-Hamet & H. Perrier. Materials and methods: The methanolic fraction was extracted from the plant roots and contained 0.48 mg bufadienolides/mg of dry mass (11a,19-dihydroksytelocinobufagin, bersaldegenin-1-acetate, bersaldegenin-1,3,5-orthoacetate, 19-(acetyloxy)-3b,5b,11a,14-tetrahydroxyl-12-oxo-bufa-20,22-dienolide and 19-(acetyloxy)-1b,3b,5b,14-tetrahydroxyl-bufa-20,22-dienolide, mainly). The cytotoxicity of K. daigremontiana fraction was evaluated in an in vitro experimental model of blood platelets. The viability of blood platelets was determined on the basis of a release of lactate dehydrogenase. Results: The fraction scavenged DPPH radicals, with EC 50 of 21.80 lg/mL. Studies on an experimental model of blood plasma under peroxynitrite-induced oxidative stress revealed that the plant preparation had moderate antioxidant properties. Levels of 3-nitrotyrosine and thiol groups indicated that the protective effect of K. daigremontiana was significant mainly for its concentration of 50 lg/mL. No effect was found in prevention of oxidation of low-molecular plasma thiols (glutathione, cysteine and cysteinylglycine). Simultaneously, measurements of lipid hydroperoxides and thiobarbituric acid-reactive substances (TBARS) indicated that the examined fraction might be effective antioxidant at broader concentration range, that is 1-5 and 25-50 lg/mL for hydroperoxides and TBARS generation, respectively. No cytotoxicity was observed at the concentration range of 1-50 lg/mL. Conclusions: Based on the obtained results, we suggest that antioxidant activity may additionally contribute to beneficial properties of K. daigremontiana-derived extracts.ARTICLE HISTORY

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“…Liu et al (1989) melaporkan adanya 7-hidroksikumarin dari K. gracillis, dan 59 senyawa flavonoid dari sembilan spesies tumbuhan Kalanchoe berhasil diisolasi (Liu et al, 1989;Costa et al, 2008). Bufadienolida merupakan metabolit sekunder yang telah diisolasi dari enam spesies genus Kalanchoe (Anderson et al, 1983;Wagner et al, 1985;Supratman et al, 2000;Yamagishi et al, 1989;Wu et al, 2006;Kuo et al, 2008). Metabolit sekunder lainnya dilaporkan sebagai asam fenolat dan megastigman dari ekstrak metanol K. hybrida dan ekstrak etil asetat K. marmorata, sedangkan sterol, triterpen, dan fenantren diisolasi dari daun segar K. pinnata dan K. thrysiflora (Milad et al, 2014).…”

Section: Pendahuluanunclassified

Senyawa Fenolik dari Daun Tanaman Kalanchoe prolifera (Crassulaceae)

Yun

Aisyah

Saputra

et al. 2017

JKV

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AbstrakSenyawa fenolik yang terdiri dari kaemferol (1), kuersetin (2), dan metil kafeat (3) telah diisolasi dari ekstrak etil asetat Kalanchoe prolifera (Crassulaceae). Struktur kimia dari senyawa yang diisolasi 1-3 dielusidasi menggunakan spektroskopi (UV, IR, NMR), dan dibandingkan melalui literatur yang telah dipublikasikan. AbstractPhenolic compounds such as kaempferol (1), quercetin (2), and methyl caffeate (3) have been isolated from the ethyl acetate extract of Kalanchoe prolifera (Crassulaceae). The chemical structure of isolated compounds 1-3 were elucidated on the basis of spectroscopic evidence (UV, IR, NMR) and comparison with those compound previouly reported.

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Kalanchosides A—C, New Cytotoxic Bufadienolides from the Aerial Parts of Kalanchoe gracilis.

Abstract: ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract, please click on HTML or PDF.

Cited by 12 publications

References 1 publication

Antioxidant, Anti-inflammatory and Antiproliferative Activities of Kalanchoe gracilis (L.) DC Stem

Antioxidant, Anti-inflammatory and Antiproliferative Activities of Kalanchoe gracilis (L.) DC Stem

Antioxidant efficacy of Kalanchoe daigremontiana bufadienolide-rich fraction in blood plasma in vitro

Senyawa Fenolik dari Daun Tanaman Kalanchoe prolifera (Crassulaceae)

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