Kaempferol in Red and Pinto Bean Seed (<i>Phaseolus vulgaris</i> L.) Coats Inhibits Iron Bioavailability Using an in Vitro Digestion/Human Caco-2 Cell Model

Hu, Ying; Cheng, Zhiqiang; Heller, Larry I; Krasnoff, Stuart B.; Glahn, Raymond P.; Welch, Ross M.

doi:10.1021/jf0612981

Cited by 63 publications

(51 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As breeding genotypes stood out FT Nobre and TB 02-11, both black grains, demonstrating that the distribution was dependent on the genotype in question, though the materials of red beans have excelled toward the other. According to Hu et al (2006), the highest astragaline levels were observed in grains with coat not black. In the analysis of the levels of antioxidant, unlike the contents of macro and micronutrients, the astragalina was more expressive in grains not black.…”

Section: Resultsmentioning

confidence: 98%

Chemical Composition of Whole Grains in Common Beans Landraces and Breeding Genotypes

Bevilaqua

Antunes

2015

HOLOS

View full text Add to dashboard Cite

The common bean has been object of breeding programs aiming the development of new cultivars adapted to varied production system and shown differentiated nutritional characteristics. Due a genetic diversity existent the landraces can be used directly for cropping, for present characteristics desirable. Little information exists about mineral content and other quality traits for those bean landraces. The aim of this paper was to verify the variability for grain nutritional characteristics in breeding cultivars and landraces of bean from Rio Grande do Sul state, Brazil. The experiment was conducted in 2009/2010 in Experimental Station Cascata, of Embrapa Temperate Agriculture. In whole grain of 54 bean genotypes with black and no black coat were determined macroelements (nitrogen, phosphorus, potash, calcium, magnesium and sulfur), oligoelements (iron, manganese, zinc and cuprum), protein and ash content, insoluble fiber, digestive nutrient and antioxidant astragalina. The results shown that the landraces varieties presents nutritional composition of macro and oligoelements, fibers, protein and ash contents in whole grain similar than that of breeding lines and cultivars. The black coat grain from breeding programs showed better nutritional quality for macro and oligoelements content than coloured grain, highlighting TB 02-04 e TB 01-01. The landraces with coloured grains TB 02-26, TB 02-24 and TB 03-13 showed the high levels of astragaline. KEYWORDS:

show abstract

Section: Resultsmentioning

confidence: 98%

Chemical Composition of Whole Grains in Common Beans Landraces and Breeding Genotypes

Bevilaqua

Antunes

2015

HOLOS

View full text Add to dashboard Cite

show abstract

“…Hu et al (2006) showed that the polyphenol, kaempferol in the pigmented bean seed coat inhibited FEBIO.…”

Section: Discussionmentioning

confidence: 99%

“…Seeds with pigmented seed coat contained much greater concentrations of polyphenols than seeds with non-pigmented seed coat. In in vitro studies, seeds with pigmented seed coat or dark-colored fruits had a negative effect on iron bioavailability (Ariza-Nieto et al, 2007;Boato et al, 2002;Hu et al, 2006). Compared with the negative effect caused by phytate which can be reversed substantially by ascorbic acid (AA), this negative effect, caused by polyphenols, was barely reversed by AA (Engle-Stone et al, 2005).…”

Section: Anti-nutritional Factorsmentioning

confidence: 99%

See 1 more Smart Citation

Iron Bioavailability in Low Phytate Pea

et al. 2015

Self Cite

View full text Add to dashboard Cite

Field pea (Pisum sativum L.) seeds have high nutritional value but also contain phytate which can inhibit the absorption and utilization of nutrients. Phytate is the main storage form of phosphorus in the seeds but chelates Fe, Zn and some other micronutrients and is not well digested by monogastrics. Peas with pigmented seed coats contain polyphenols which also have anti-nutritional properties. To increase the nutritional value of field pea seeds, two low phytate lines (1-150-81 and 1-2347-144) containing higher inorganic phosphorus concentration (IN-P) and lower phytate-phosphorus concentration (PA-P) than the normal phytate varieties were developed from CDC Bronco in previous research. The objectives of this research were 1) to determine the effect of genotype and environment on iron bioavailability in a set of five pea varieties differing in phytate concentration and iron concentration using in vitro digestion/Caco-2 cell culture bioassay; 2) to determine the effect of seed coats on iron bioavailability by testing whole seeds compared to dehulled seeds in varieties differing in seed coat pigmentation using in vitro digestion/Caco-2 cell culture bioassay; 3) to determine the inheritance of iron bioavailability in field pea by evaluating recombinant inbred lines differing in phytate concentration using in vitro digestion/Caco-2 cell culture bioassay; 4) to determine the effects of pea with the low phytate trait on body weight and hemoglobin concentration of chickens. Iron concentration (FECON) did not differ significantly between normal and low phytate varieties. Iron bioavailability (FEBIO) of the two low-phytate lines was 1.4 to 1.9 times higher than that of the three normal phytate varieties, and growing environment also had a significant effect on FEBIO. Peas with pigmented seed coats contained 7 times lower FEBIO than peas with non-pigmented seed coat. The removal of the seed coat increased the FEBIO in peas with pigmented seed coat 5 to 6 times. From previous research on PR-15 recombinant inbred lines (RILs) which were developed from a cross between low phytate line 1-2347-144 and a normal phytate variety CDC Meadow, it was found that PA-P was controlled by a single gene. FEBIO, in this study, was also found to follow a bimodal frequency distribution, characteristic of single gene iii control, and it was highly correlated with PA-P in the PR-15 lines. In vivo studies were used to evaluate iron absorption of chickens fed with low and normal phytate pea diets. The diets containing the low-phytate pea lines had no significant effect on chicken body weight and hemoglobin level, compared with the diets containing normal phytate pea varieties. An unexpected high FECON was discovered in the diets that was traced to the ingredients of limestone and dicalcium-phosphate which likely affected the experimental results.iv

show abstract

“…Among them, Phaseolus vulgaris (L.) is the most important staple food crop in several countries, providing significant amounts of protein, fibers, minerals and vitamins (Hu et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

Phytogeographical origin of Madeiran common beans based on phaseolin patterns

Silva

Correia

Lopes

et al. 2010

Pesq. agropec. bras.

View full text Add to dashboard Cite

-The objective of this work was to determine the geographic origin of the Madeiran common bean (Phaseolus vulgaris) gene pool. Phaseolin patterns of 50 accessions representing the diversity of common bean collected in Madeira, Portugal, and conserved in the ISOPlexis Germplasm Bank, were analysed using the Experion automated electrophoresis system, based on lab-on-a-chip technology. Five common bean standard varieties with typical phaseolin patterns were used to determine the phytogeographical origin of the Madeiran common bean accessions. Ninety two percent of the accessions exhibited a phaseolin pattern consistent with the one of common bean types belonging to the Andean gene pool, while the origin of the remaining 8% of the accessions was indistinguishable. The application of a similarity coefficient of 85%, based on Pearson correlations, increases the number of accessions with uncertain pattern. The analytical approach used permitted the determination of the origin of the common bean gene pool, which is Andean in 98% of the cases, and clustering of the observed variability among the Madeiran common beans.

show abstract

Kaempferol in Red and Pinto Bean Seed (Phaseolus vulgaris L.) Coats Inhibits Iron Bioavailability Using an in Vitro Digestion/Human Caco-2 Cell Model

Cited by 63 publications

References 30 publications

Chemical Composition of Whole Grains in Common Beans Landraces and Breeding Genotypes

Chemical Composition of Whole Grains in Common Beans Landraces and Breeding Genotypes

Iron Bioavailability in Low Phytate Pea

Phytogeographical origin of Madeiran common beans based on phaseolin patterns

Contact Info

Product

Resources

About