-The objective of this work was to determine the geographic origin of the Madeiran common bean (Phaseolus vulgaris) gene pool. Phaseolin patterns of 50 accessions representing the diversity of common bean collected in Madeira, Portugal, and conserved in the ISOPlexis Germplasm Bank, were analysed using the Experion automated electrophoresis system, based on lab-on-a-chip technology. Five common bean standard varieties with typical phaseolin patterns were used to determine the phytogeographical origin of the Madeiran common bean accessions. Ninety two percent of the accessions exhibited a phaseolin pattern consistent with the one of common bean types belonging to the Andean gene pool, while the origin of the remaining 8% of the accessions was indistinguishable. The application of a similarity coefficient of 85%, based on Pearson correlations, increases the number of accessions with uncertain pattern. The analytical approach used permitted the determination of the origin of the common bean gene pool, which is Andean in 98% of the cases, and clustering of the observed variability among the Madeiran common beans.
The processing of fruits produces a high amount of waste, which can result in being a form of environmental pollution, and may cause health risks. However, fruit wastes are sources of high nutritional level compounds, which can be used to prepare nutritious food products, the source of low-cost nutrients. Given the above, the present work aimed to produce a shake beverage from tropical fruit wastes, limiting the environmental impact and adding nutritional value to the waste. The fruit wastes used were: orange, lemon, guava, banana, mango, acerola, passion fruit and tangerine. Six shake formulations were prepared by varying the wastes used in each. Chemical and physicochemical analyses of the wastes and the shakes were carried out. In addition, microbiological analyses and sensorial acceptance tests were performed on the shake. The shake had good amounts of ascorbic acid and low lipid content. Evaluating the results of the sensorial acceptance tests, it was noticed that the product, for the most part, was well accepted by the tasters, and the highest marks were awarded by the tasters who declared they practiced sports regularly. Thus, the different developed shake formulations have the potential to be introduced in the market.
<p>Various drying techniques can be applied, depending on the raw material you want to dehydrate and what you want to obtain. One way to assess whether the dehydration method was efficient is through sorption isotherms. The objective of the work was to analyze and compare the adsorption isotherms of the cajá pulp powder obtained by atomization, with the use of maltodextrin (Test A) and maltodextrin and soy lecithin (Test B). The mathematical models of GAB, BET, Henderson and Oswin were used to represent the isotherms at 25, 30, 35 and 40 ° C. The results showed that the addition of lecithin gives a statistically different powder (p <0.05) from a powder without addition in relation to soluble solids, total sugars and its color. The BET model was the one that best fit Test A, and the Henderson model was the one that best fit Test B, which proved to be Type III in the J format. It was also observed that the addition of soy lecithin provided greater water adsorptio, which demonstrates that the product should be stored in a location with low levels of relative humidity or in packaging with low permeability.</p>
The goal of this work was to compare reproducibility of phaseolin patterns of common bean obtained by two electrophoretic protein separation techniques including the conventional SDS-PAGE and an automated chip electrophoresis system. Five standard cultivars of common bean provided by the United States Department of Agriculture (Beltsville, Maryland) that represented five phaseolin types, T (Tendergreen), C (Contender) and S (Sanilac), B (Boyaca) and P (Pampa), were used in this study. Comparison of the phaseolin patterns revealed that the chip-on-a-lab electrophoresis provided a good reproducibility. The phaseolin polymorphism included four to seven polypeptides typical for the pattern composition of the T, C and S types. The polymorphism of the B and P patterns was also established. Phaseolin polypeptides separated by the microchip electrophoresis exhibited differences with respect to the molecular weights and electrophoretic mobility as compared to the SDS-PAGE technique. This phenomenon could be attributed to the absence of a solid separation phase in the microchip electrophoresis. Moreover, this technique has potential to substantially accelerate screening of large bean germplasm collections since it allows for the accurate analysis of the higher number of individual plants within accessions than the conventional, tedious and time consuming SDS-PAGE method.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.