Kaempferitrin inhibits colorectal cancer cells by inducing reactive oxygen species and modulating PI3K/AKT signalling pathway

Govindarasu, Mydhili; Abirami, Pari; Rajakumar, Govindasamy; Ansari, Mohammad Azam; Alomary, Mohammad N.; Alkhayl, Faris F. Aba; Aloliqi, Abdulaziz A.; Thiruvengadam, Muthu; Vaiyapuri, Manju

doi:10.1016/j.procbio.2022.02.021

Cited by 3 publications

(2 citation statements)

References 58 publications

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“…A total of eight compounds, including flavonoids, anthraquinones, and triterpenoids, were identified from the HSF of KR extracts via LC-MS analysis ( Table 2 ). Among these, calycosin, aloe emodin, rhein, and maackiain reportedly exhibit effects against breast cancer [ 29 , 30 , 31 ], while asperuloside, orientin, vicenin-2, and kaempferitrin were proven to exhibit anti-tumor activity [ 32 , 33 , 34 , 35 , 36 ]. Additionally, 3-hydroxymorindone and rubiadin were found to occur in HSF through a comparison with reference compounds.…”

Section: Resultsmentioning

confidence: 99%

Extracts of Knoxia roxburghii (Spreng.) M. A. Rau Induce Apoptosis in Human MCF-7 Breast Cancer Cells via Mitochondrial Pathways

Chen

et al. 2022

Molecules

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Knoxia roxburghii (Spreng.) M. A. Rau (KR) is a plant clinically used in traditional Chinese medicine (TCM) for the treatment of cancer. The study objectives were to examine the effects of KR extracts, petroleum ether (PET), ethyl acetate (EtoAc), butanol (n-BuOH), and H2O-soluble fractions (HSF) of the 75% EtOH extraction on A549 (non-small cell lung cancer), HepG2 (liver cancer), HeLa (cervical cancer), MCF-7 (breast cancer), and L02 (normal hepatocyte) cells. It was found that HSF exhibited the strongest cytotoxic activity against MCF-7 cells, and was accompanied by reduced mitochondrial transmembrane potential, increased levels of intra-cellular reactive oxygen species (ROS) and activated caspases, and upregulated pro-apoptotic and downregulated anti-apoptotic proteins. LC-MS analysis further showed that HSF primarily consisted of calycosin, aloe emodin, rein, maackiain, asperuloside, orientin, vicenin-2, and kaempferide, which have been mostly reported for anti-tumor activity in previous studies. In summary, the current study illustrated the effect, mechanism, and the potential major active components of KR against breast cancer.

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Section: Resultsmentioning

confidence: 99%

Extracts of Knoxia roxburghii (Spreng.) M. A. Rau Induce Apoptosis in Human MCF-7 Breast Cancer Cells via Mitochondrial Pathways

Chen

et al. 2022

Molecules

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“…The cytotoxicity assay of green synthesized P. guajava Co 3 O 4 NPs was performed using the previously reported method [ 68 ]. Vero, MCF-7 and HCT 116 cell lines were plated in 96 well plates with 1 × 10 5 cells/well in DMEM medium containing 10% Fetal bovine serum (FBS).…”

Section: Methodsmentioning

confidence: 99%

Green Synthesis and Characterization of Cobalt Oxide Nanoparticles Using Psidium guajava Leaves Extracts and Their Photocatalytic and Biological Activities

et al. 2022

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The advanced technology for synthesizing nanoparticles utilizes natural resources in an environmentally friendly manner. Additionally, green synthesis is preferred to chemical and physical synthesis because it takes less time and effort. The green synthesis of cobalt oxide nanoparticles has recently risen due to its physico-chemical properties. In this study, many functional groups present in Psidium guajava leaf extracts are used to stabilize the synthesis of cobalt oxide nanoparticles. The biosynthesized cobalt oxide nanoparticles were investigated using UV-visible spectroscopic analysis. Additionally, Fourier-transform infrared spectroscopy revealed the presence of carboxylic acids, hydroxyl groups, aromatic amines, alcohols and phenolic groups. The X-ray diffraction analysis showed various peaks ranging from 32.35 to 67.35°, and the highest intensity showed at 36.69°. The particle size ranged from 26 to 40 nm and confirmed the average particle size is 30.9 nm. The green synthesized P. guajava cobalt oxide nanoparticles contain cobalt as the major abundant element, with 42.26 wt% and 18.75 at% confirmed by the EDAX techniques. SEM images of green synthesized P. guajava cobalt oxide nanoparticles showed agglomerated and non-uniform spherical particles. The anti-bacterial activity of green synthesized P. guajava cobalt oxide nanoparticles was evaluated against Gram-positive Staphylococcus aureus and Gram-negative Escherichia coli with a 7 to 18 mm inhibitory zone. The photocatalytic activity was evaluated using green synthesized P. guajava cobalt oxide nanoparticles and observed 79% of dye degradation. The MTT assay of P. guajava cobalt oxide nanoparticles showed an excellent cytotoxic effect against MCF 7 and HCT 116 cells compared to normal cells. The percentage of cell viability of P. guajava cobalt oxide nanoparticles was observed as 90, 83, 77, 68, 61, 58 and 52% for MCF-7 cells and 82, 70, 63, 51, 43, 40, and 37% for HCT 116 cells at the concentration of 1.53, 3.06, 6.12, 12.24, 24.48, 50, and 100 μg/mL compared to control cells. These results confirmed that green synthesized P. guajava cobalt oxide nanoparticles have a potential photocatalytic and anti-bacterial activity and also reduced cell viability against MCF-7 breast cancer and HCT 116 colorectal cancer cells.

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Phytochemical, Pharmacological, and Toxicological Prospection of Morus nigra L.: A Systematic Review

Alves

Santos

Souza

et al. 2024

NPJ

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INTRODUCTION: Morus nigra L. has industrial relevance due to the presence of specialized metabolites, which possess pharmaceutical potential in various parts and preparations. This review presents updated information on traditional, phytochemical, and pharmacological applications, as well as toxicity data, pertaining to different parts of Morus nigra L.. METHOD: Phytochemical research and ethnobotanical studies were conducted using reviewed databases. Mulberry leaves have demonstrated several biological activities, attributed to the presence of phenolic acids, flavonoids, and fatty acids. Stems and roots contain additional compounds such as stilbenes and benzofurans. Morus nigra L. exhibits various biological activities, including hepatoprotective, hypolipidemic, anti-inflammatory, antioxidant, antimicrobial, neuroprotective, hypoglycemic, skin whitening, cytotoxic, antiatherosclerotic, and antiobesity effects. The choice of extraction technique and plant part is crucial to obtain a diverse range of compounds necessary for specific indications RESULT: Accelerated solvent extraction (ASE) has proven to be the most advantageous method compared to supercritical fluid maceration and extraction (SFE), yielding a wide variety of compounds. CONCLUSION: Overall, this review aims to provide scientists and companies interested in Morus nigra L. with opportunities and challenges for innovation in this field.

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Kaempferitrin inhibits colorectal cancer cells by inducing reactive oxygen species and modulating PI3K/AKT signalling pathway

Cited by 3 publications

References 58 publications

Extracts of Knoxia roxburghii (Spreng.) M. A. Rau Induce Apoptosis in Human MCF-7 Breast Cancer Cells via Mitochondrial Pathways

Extracts of Knoxia roxburghii (Spreng.) M. A. Rau Induce Apoptosis in Human MCF-7 Breast Cancer Cells via Mitochondrial Pathways

Green Synthesis and Characterization of Cobalt Oxide Nanoparticles Using Psidium guajava Leaves Extracts and Their Photocatalytic and Biological Activities

Phytochemical, Pharmacological, and Toxicological Prospection of Morus nigra L.: A Systematic Review

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