Extracts of Knoxia roxburghii (Spreng.) M. A. Rau Induce Apoptosis in Human MCF-7 Breast Cancer Cells via Mitochondrial Pathways

Chen, Xiao-Jiao; Pu, Xin-Ying; Pu, Xue-Mei; Li, Xue; Liu, Zhibo; Mei, Mi-Jia; Wang, Xin-Ge; Zhang, Fan; Qiu, Bin; Yu, Jie

doi:10.3390/molecules27196435

Cited by 7 publications

(4 citation statements)

References 57 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…K. valerianoides), known locally as 'Zi Daji, ' is a perennial herb that is a member of the madder (Rubiaceae) family. It is cultivated in different areas of China and recognized for its medicinal properties in traditional Chinese medicine (TCM) [1]. However, root rot is a common disease that affects the cultivation of various root and rhizome medicinal plants, and it has been reported on numerous occasions [2,3].…”

Section: Objectivementioning

confidence: 99%

Genome sequence of Fusarium oxysporum strain ByF01, the causal agent of root rot of Knoxia roxburghii in China

Liu,

Guo,

Zhang

et al. 2024

BMC Genom Data

Self Cite

View full text Add to dashboard Cite

Objectives Knoxia roxburghii is a member of the madder (Rubiaceae) family. This plant is cultivated in different areas of China and recognized for its medicinal properties, which leads to its use in traditional Chinese medicine. The incidence of root rot was 10–15%. In June 2023, the causal agent of root rot on K. roxburghii was identified as Fusarium oxysporum. To the best of our knowledge, this is the first report of the complete genome of F. oxysporum strain ByF01 that is the causal agent of root rot of K. roxburghii in China. The results will provide effective resources for pathogenesis on K. roxburghii and the prevention and control of root rot on this host in the future. Data description To understand the molecular mechanisms used by F. oxysporum to cause root rot on K. roxburghii, strain ByF01 was isolated from diseased roots and identified by morphological and molecular methods. The complete genome of strain ByF01 was then sequenced using a combination of the PacBio Sequel IIe and Illumina sequencing platforms. We obtained 54,431,725 bp of nucleotides, 47.46% GC content, and 16,705 coding sequences.

show abstract

Section: Objectivementioning

confidence: 99%

Genome sequence of Fusarium oxysporum strain ByF01, the causal agent of root rot of Knoxia roxburghii in China

Liu,

Guo,

Zhang

et al. 2024

BMC Genom Data

Self Cite

View full text Add to dashboard Cite

show abstract

“…The root is used to treat diarrhea, eliminate swellings and disperse knots among others ( Liu et al, 2023b ). Its radix has diuretic, anti-inflammatory, and antitumor effects ( Chen et al, 2022 ). This plant is primarily distributed in southern China and Southeast Asia ( Zhang et al, 2023 ).…”

Section: Introductionmentioning

confidence: 99%

“…Many anthraquinones have displayed anti-tumor, antibiotic and insecticidal activities ( Dikkala et al, 2023 ). Anthraquinones are also an important source of anti-tumor drugs ( Chen et al, 2022 ). With the increasing incidence of cancer and the modernization of TCM, the demand for K. roxburghii root materials is increasing.…”

Section: Introductionmentioning

confidence: 99%

Structure and function of rhizosphere soil microbial communities associated with root rot of Knoxia roxburghii

Liu,

Li,

Dong

et al. 2024

Front. Microbiol.

Self Cite

View full text Add to dashboard Cite

The microbial communities in rhizosphere soil play important roles in plant health and crop productivity. However, the microbial community structure of rhizosphere soil still remains unclear. In this study, the composition, diversity and function of the microbial communities in the rhizosphere soil of healthy and diseased plants were compared using Illumina MiSeq high-throughput sequencing. The Sobs (richness) and Shannon (diversity) indices of the soil microbial communities were higher in the rhizospheres of 2- and 3-year-old susceptible plants than in those of the healthy plants. With the increase in planting time, the numbers of fungi tended to decrease, while those of the bacteria tended to increase. Fungal diversity could be used as a biological indicator to measure the health of Knoxia roxburghii. The microbial composition and differential analyses revealed that the rhizosphere soil infested with fungi had a higher relative abundance at the phylum level in Ascomycota and Basidiomycota, while the bacteria had a higher relative abundance of Chloroflexi and a lower relative abundance of Actinobacteriota. At the genus level, the rhizosphere soil infested with fungi had relatively more abundant unclassified_f__Didymellaceae and Solicoccozyma and relatively less abundant Saitozyma and Penicillium. The bacterial genus norank_f__Gemmatimonadaceae was the most abundant, while Arthrobacter was less abundant. In addition, the abundance of Fusarium in the fungal community varied (p = 0.001). It tended to increase in parallel with the planting years. Therefore, it was hypothesized that the change in the community composition of Fusarium may be the primary reason for the occurrence of root rot in K. roxburghii, and the change in the abundance of Fusarium OTU1450 may be an indication of the occurrence of root rot in this species. The community function and prediction analyses showed that the pathogenic fungi increased with the increase in planting years. In general, soil fungi can be roughly divided into three types, including pathotrophs, symbiotrophs, and saprotrophs. An analysis of the differences in the prediction of different rhizosphere functions showed that D and L were significantly different in the COG enrichment pathway of the K. roxburghii rhizosphere bacteria (p < 0.05). The soil physical and chemical properties, including the pH, AK, total potassium (TK), and catalase (S_CAT), had the most significant effect on the soil fungal community, and most of the soil physical and chemical properties significantly correlated with the bacterial community. This study demonstrated that the occurrence of root rot had an important effect on the diversity, structure and composition of microbial communities. In addition, the results will provide a theoretical basis to prevent and control root rot in K. roxburghii.

show abstract

“…In recent years, there is a growing body of literature emphasizing the role of plant-derived natural products and plant extracts in support of cancer treatments via activation of apoptosis [ 2 , 5 , 6 , 7 ].…”

Section: Introductionmentioning

confidence: 99%

Iodine-Biofortified Lettuce Can Promote Mitochondrial Dependent Pathway of Apoptosis in Human Gastrointestinal Cancer Cells

Sularz

Koronowicz

Smoleń

et al. 2023

IJMS

View full text Add to dashboard Cite

Previously, our research provided evidence that exposure of gastric and colon cancer cells to extracts from iodine-biofortified lettuce leads to a reduction of cell viability and proliferation through cell cycle arrest and upregulation of pro-apoptotic genes. The aim of the present study was to determine the potential cellular mechanisms of induction of cell death in human gastrointestinal cancer cell lines after treatment with iodine-biofortified lettuce. We demonstrated that extracts from lettuce enriched with iodine induce apoptosis in gastric AGS and colon HT-29 cancer cells and the mechanism of programmed cell death may be triggered and executed through different signaling pathways, depending on the type of cells. Western blot analysis revealed that iodine-fortified lettuce leads to cell death through the release of cytochrome c to the cytosolic fraction and activation of the primary drivers of apoptosis: caspase-3, caspase-7, and caspase-9. Furthermore, we have reported that apoptotic effects of lettuce extracts may be mediated by poly (ADP-ribose) polymerase (PARP) and activation of pro-apoptotic Bcl-2 family proteins such as Bad, Bax, and BID. We also observed mitochondrial dysfunction with the dissipation of the mitochondrial membrane potential in cells exposed to lettuce extracts. Taken together, these results indicate that the organic form of iodine such as 5-ISA and 3,5-diISA is an important factor in the activation of intrinsic mitochondrial apoptotic pathway in AGS and HT-29 cancer cells in a p53-independent manner.

show abstract

Extracts of Knoxia roxburghii (Spreng.) M. A. Rau Induce Apoptosis in Human MCF-7 Breast Cancer Cells via Mitochondrial Pathways

Cited by 7 publications

References 57 publications

Genome sequence of Fusarium oxysporum strain ByF01, the causal agent of root rot of Knoxia roxburghii in China

Genome sequence of Fusarium oxysporum strain ByF01, the causal agent of root rot of Knoxia roxburghii in China

Structure and function of rhizosphere soil microbial communities associated with root rot of Knoxia roxburghii

Iodine-Biofortified Lettuce Can Promote Mitochondrial Dependent Pathway of Apoptosis in Human Gastrointestinal Cancer Cells

Contact Info

Product

Resources

About