K cell mediated lysis of cultured colon carcinoma and urinary bladder carcinoma cells induced by monospecific antisera against carcinoembryonic antigen (CEA) and two CEA‐related normal glycoproteins

Hammarström, Sten; Troye, Marita; Wahlund, G.; Svenberg, T.; Perlmann, Peter

doi:10.1002/ijc.2910190604

Cited by 14 publications

(13 citation statements)

References 35 publications

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“…That this was not due to a lesser susceptibility to lysis in ADCC of the HT29 cells is shown by the results obtained with IgG from the untreated patients with carcinoma of the prostate. If anything, the latter preparations tended to be more lytic to HT29 than to T24, although this difference was not statistically significant, The present findings are also in the line with those obtained by testing these two target cells in ADCC induced by rabbit antiserum IgG, specific either for "24 or for CEA (carcinoembryonic antigen), produced by HT29 but not by T24 (Hammarstrom et al, 1977;Schneider et al, 1980). IgG from treated TCC patients reacted similarly to that from untreated patients although the difference in lysis between the two target cells was less pronounced.…”

Section: Discussionsupporting

confidence: 91%

Lymphocyte‐mediated lysis of tumor cells in vitro (ADCC), induced by serum antibodies from patients with urinary bladder carcinoma or from controls

Troye

Hansson

Paulie

et al. 1980

Intl Journal of Cancer

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IgG fractions from serum of patients with transitional-cell carcinoma of the urinary bladder (TCC), patients with carcinoma of the prostate (CC) and healthy donors (HD) were tested for their capacity to induce antibody-dependent lymphocyte-mediated cytotoxicity (ADCC) to tumor cells in vitro. Lymphocytes from healthy donors were selected for low natural cytotoxicity to the target cells from established cell lines of TCC or other origins. IgG was prepared by adsorption of serum to Sepharose-bound protein A from Staphylococcus aureus and subsequent acid elution. When tested against a panel of six different target cells, most individual IgG preparations from all three donor groups contained antibodies inducing ADCC to some of the target cells. When IgG preparations from II untreated TCC patients were studied for ADCC induction to the TCC target T24 and the colon carcinoma HT29, cytotoxicity to T24 was, on an average, significantly higher than that to HT29. For IgG preparations from 18 TCC patients, treated with radiotherapy, a similar difference was seen but was not statistically significant. IgG preparations from II patients with carcinoma of the prostate and from 12 healthy donors did not show this differences. Moreover, while individual IgG preparations from untreated TCC patients were, on the average, significantly more cytotoxic to T24 than those from either of the two control groups, no such differences were seen when HT29 was the target. On the contrary, IgG preparations from patients with prostatic carcinoma were significantly more cytotoxic to HT29 than those from healthy donors. The results suggest that TCC patients develop a disease-related humoral immune response, superimposed on a "natural" immunity to a variety of antigens on the target cells used. The nature of the antigens involved in these reactions remains to be established. However, the results are compatible with previous findings, in these patients, of a bladder-tumor-related cellular cytotoxicity, to a large extent caused by the patients' own antibodies.

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Section: Discussionsupporting

confidence: 91%

Lymphocyte‐mediated lysis of tumor cells in vitro (ADCC), induced by serum antibodies from patients with urinary bladder carcinoma or from controls

Troye

Hansson

Paulie

et al. 1980

Intl Journal of Cancer

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“…Conversely, rabbit antisera to each of these control tumor cells induced a stronger cell-mediated lysis of their homologous targets than of the bladder tumor cells (see also Hammarstrom et al, 1977). This specificity of aT-24 was fully retained after absorption with fetal bovine serum.…”

Section: Discussionmentioning

confidence: 90%

“…The specificity of the sera was assessed by antibody-dependent lymphocyte-mediated cytotoxicity (ADCC) which provides a sensitive and relevant assay system for the analysis of MAA (Hammarstrom et al, 1977). In this paper the production of an anti-TCC serum and its specificity are described.…”

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confidence: 99%

Membrane‐associated antigens on tumor cells from transitional‐cell carcinoma of the human urinary bladder. I. Immunological characterization by xenogeneic antisera

Schneider

Troye

Paulie

et al. 1980

Intl Journal of Cancer

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An antiserum was raised in rabbits by immunization with a human tumor cell line, T-24, derived from transitional cell carcinoma (TCC) of the urinary bladder. The specificity of the IgG fraction was assessed by antibody-dependent cellular cytotoxicity (ADCC), using purified blood lymphocytes from healthy human donors as effector cells and seven human cell lines as target cells (T-24 and two additional TCC-lines, two normal urothelial lines, one colon carcinoma and one malignant melanoma). The IgG induced strong lysis of all seven target cell types. However, lysis of the five urothelial lines was significantly stronger than that of the control tumors. Conversely, antisera to either of the control tumors induced a significantly stronger lysis of the homologous tumors than of the urothelial cells. All antisera contained antibodies to fetal bovine serum but removal of these by absorption did not change the specificity of the ADCC reactions. When the anti T-24 serum was exhaustively absorbed with glutaraldehyde-fixed spleen homogenate, the cytotoxicity to the control tumor targets was abolished. Although absorption reduced the antibody titer of the IgG preparation, ADCC to the TCC targets remained at a high level. There was no difference in the degree of lysis of the three TCC targets. Lysis of the two target lines from normal urothelium was slightly lower than that of the tumor cells. The results indicate that the anti-TCC serum contained antibodies to one or several antigens shared by five urothelial cell lines but not by the control tumors. Whether or not it also contained antibodies to TCC-associated antigens remains to be established. The molecular basis for these findings will be given in the accompanying paper.

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“…Although cytotox icity mediated by NK has been widely con sidered to be the better arm of defense against neoplasia [14], this has been ques tioned lately [ 15], The possibility that spon taneous tumors, like the one used here, can escape NK mechanisms [16] encouraged us to use ADCC in our model. Moreover, ADCC has a high discriminating power and is more sensitive [8],…”

Section: Discussionmentioning

confidence: 99%

“…The macrophage has a known antineoplastic ac tivity [4] capable of killing tumor cells [5] either as helper cell [6] or as a tumor cyto toxic effector cell [7], Furthermore, T lym phocytes are generally accepted to be capable of lysing tumor cells [8], crucial in tumor growth regulation [9].…”

Section: Introductionmentioning

confidence: 99%

Macrophage and Lymphocyte Antibody-Dependent Cellular Cytotoxicity in Spontaneous Leukemogenesis of AKR/J Mice

Fuente¹,

Alonso²,

Solana³

et al. 1989

Tumor Biol

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Antibody-dependent cell-mediated cytotoxicity (ADCC) mediated by peritoneal macrophages and splenic lymphocytes was studied in young (15 weeks) and old (45 ± 5 weeks) preleukemic AKR/J mice and leukemic (55 ± 5 weeks) AKR/J mice. This strain spontaneously develops a virally induced T cell leukemia-lymphoma between the ages of 32 and 48 weeks. The peritoneal macrophages from the AKR/J leukemic and old preleukemic mice showed an impaired ADCC when compared with their respective age and tumor-free BALB/c controls and with young preleukemic AKR/J mice. Young and old preleukemic AKR/J mice and their respective controls had similar ADCC values with respect to splenic lymphocytes, whereas an impaired ADCC was observed by the splenic cells from leukemic AKR/J mice. The possibility of using ADCC mediated by macrophages as a test to detect the onset of the leukemic process is discussed.

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K cell mediated lysis of cultured colon carcinoma and urinary bladder carcinoma cells induced by monospecific antisera against carcinoembryonic antigen (CEA) and two CEA‐related normal glycoproteins

Cited by 14 publications

References 35 publications

Lymphocyte‐mediated lysis of tumor cells in vitro (ADCC), induced by serum antibodies from patients with urinary bladder carcinoma or from controls

Lymphocyte‐mediated lysis of tumor cells in vitro (ADCC), induced by serum antibodies from patients with urinary bladder carcinoma or from controls

Membrane‐associated antigens on tumor cells from transitional‐cell carcinoma of the human urinary bladder. I. Immunological characterization by xenogeneic antisera

Macrophage and Lymphocyte Antibody-Dependent Cellular Cytotoxicity in Spontaneous Leukemogenesis of AKR/J Mice

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