Macrophage and Lymphocyte Antibody-Dependent Cellular Cytotoxicity in Spontaneous Leukemogenesis of AKR/J Mice

Fuente, Mónica De la; Alonso, M. C.; Solana, Rafael; Peña, José

doi:10.1159/000217630

Cited by 8 publications

(3 citation statements)

References 14 publications

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“…mice [21,22]. Our data support the concept that in nude mice ADCC mediated by polyclonal or monoclonal antibodies directed against extracellular melanoma antigens can direct immune cells from nude mice (including NK cells, macrophages, and possibly others) to play an important role in tumor rejection [20,23]. The ADCC assay could be considered an in vitro model for in vivo experiments in which melanoma cell challenge took place following pretreatment with specific antibody, and effector cells (NK and macrophages) were attached to melanoma cells from the blood and lymphatics.…”

Section: Discussionsupporting

confidence: 79%

“…It has been shown that NK cells and macrophages functioned as effector cells when combined with treatment of mice with a mAb, BR96, which recognizes a cell membrane antigen of a cultured human breast cancer and had cytotoxic activity in vitro and in vivo in a nude mice model [20,23]. The ADCC assay could be considered an in vitro model for in vivo experiments in which melanoma cell challenge took place following pretreatment with specific antibody, and effector cells (NK and macrophages) were attached to melanoma cells from the blood and lymphatics.…”

Section: Discussionmentioning

confidence: 99%

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Nude mouse model to study passive humoral immunotherapy directed against B16 F10 murine melanoma

Shrayer

Bogaars

Gersten

et al. 1994

Journal of Surgical Oncology

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A model to study passive humoral immunotherapy of experimental melanoma was generated by subcutaneous injection of B16 F10 murine melanoma cells in the midtail of BALB/C nude (nu/nu) mice. Mice were challenged with melanoma cells pretreated: (1) with complete culture medium, (2) with 10% adjuvant control serum, (3) with 10% anti-fECA (formalinized extracellular antigens) immune serum, or (4) with a monoclonal antibody (mAB H2-3-3) specific for the B700 melanoma-associated antigen. All control mice challenged with melanoma cells pretreated either with culture medium or with medium containing adjuvant control serum (Groups I and II) died during the observation period of 84 days. At day 84, 60% of the mice challenged with melanoma cells pretreated with anti-fECA immune serum (Group III) survived, as did 100% of the mice challenged with cells pretreated with mAb H2-3-3 (Group IV). Injection of melanoma cells pretreated with mAb H2-3-3 was associated with the greatest reduction of subsequent local tumor growth and the lowest number of metastatic lung tumors. The inhibitory effects of immune sera in vivo also correlated with in vitro effects of anti-fECA immune serum and mAb H2-3-3, determined on B16 F10 melanoma target cells using assays for DNA synthesis and antibody dependent cellular cytotoxicity (ADCC). In sum, this nude mouse model for the study of passive humoral immunotherapy of experimental melanoma was utilized to demonstrate significant protective effects against B16 F10 melanoma cell challenge by treatment with anti-fECA immune sera or a melanoma-specific monoclonal antibody.

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Section: Discussionsupporting

confidence: 79%

Section: Discussionmentioning

confidence: 99%

Nude mouse model to study passive humoral immunotherapy directed against B16 F10 murine melanoma

Shrayer

Bogaars

Gersten

et al. 1994

Journal of Surgical Oncology

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“…The ADCC assay used was that described by De la Fuente et al (1989). Briefly, target cells were labeled with slCr as detailed above, then washed 3 times with culture medium containing 5% FBS.…”

Section: Cytotoxicity Assaysmentioning

confidence: 80%

Generation of cytotoxic antibodies to the B16 murine melanoma using a formalinized vaccine

Shrayer

Kouttab

Maizel

et al. 1993

Intl Journal of Cancer

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The goal of our experiments was to determine the extent to which the humoral response to a melanoma vaccine elicits the production of cytotoxic antibodies in tumor-challenged mice. Mice were immunized with a vaccine produced from formalinized extracellular antigens (FECA) derived from B16 F10 melanomas. The production of antibodies that recognized the vaccine preparation was determined by ELISA, as was their cross-reactivity with the B700 melanoma antigen. The antibodies were shown to be anti-proliferative by inhibition of tritiated thymidine incorporation into the DNA of cultured target cells and cytotoxic by assays for complement-mediated and antibody-dependent cellular cytotoxicity. Flow cytometric analyses indicated that approximately 60% of the target cells specifically bound antibody from the immune sera. These results confirm that B700 is a significant antigenic component of the FECA vaccine, and provide encouragement for this approach to developing useful melanoma vaccines.

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Expression of autocrine motility factor receptor in serum‐ and protein‐independent fibrosarcoma cells: Implications for autonomy in tumor‐cell motility and metastasis

Watanabe

Shinozaki

Raz

et al. 1993

Intl Journal of Cancer

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The motile response of serum-dependent (Gc-4 SD) and protein-independent (Gc-4 PF) murine fibrosarcoma cells to monoclonal antibody (MAb) that binds to gp78 a cell-surface receptor (M(r) 78,000) for an autocrine motility factor (AMF) was analyzed. The Gc-4 PF cells responded to the anti-gp78 by increased motility in vitro (3-fold) and increased lung colonization in vivo (8- to 20-fold), while the serum-dependent counterpart failed to respond to motile stimulation both in vitro and in vivo. Immuno-analysis of cell-surface expression and cell extracts revealed a smaller amount of gp78 in Gc-4 SD cells than in Gc-4 PF cells. Both cell lines secrete an equal amount of AMF to the culture media. Our results suggest that protein-free culture of Gc-4 PF cells is associated with high response to AMF and with high expression of its receptor, and that autonomous motile regulation may play a role in tumor dissemination.

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Macrophage and Lymphocyte Antibody-Dependent Cellular Cytotoxicity in Spontaneous Leukemogenesis of AKR/J Mice

Cited by 8 publications

References 14 publications

Nude mouse model to study passive humoral immunotherapy directed against B16 F10 murine melanoma

Nude mouse model to study passive humoral immunotherapy directed against B16 F10 murine melanoma

Generation of cytotoxic antibodies to the B16 murine melanoma using a formalinized vaccine

Expression of autocrine motility factor receptor in serum‐ and protein‐independent fibrosarcoma cells: Implications for autonomy in tumor‐cell motility and metastasis

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