Jungia sellowii suppresses the carrageenan-induced inflammatory response in the mouse model of pleurisy

Abstract

This study was conducted to explore the anti-inflammatory effect of Jungia sellowii (Asteraceae) using a murine model of pleurisy induced by carrageenan (Cg). This plant is used in southern Brazil to treat inflammatory diseases. J. sellowii leaves were extracted with ethanol/water to obtain the crude extract (CE), which was fractionated with different solvents, yielding n-hexane (Hex), dichloromethane (DCM), ethyl acetate (EtOAc) and n-butanol (BuOH) fractions, and aqueous fraction (Aq). The major compounds succinic acid (SA) and lactic acid (LA) were isolated from Aq fraction, and their structures were determined by (1)H and (13)C NMR. Pleurisy was induced by Cg (Saleh et al. 1996). The leukocytes, exudation, myeloperoxidase (MPO) and adenosine-deaminase (ADA) activities, metabolites of nitric oxide (NO x ) levels, protein levels and mRNA expression for interleukin 1 beta (IL-1β), tumour necrosis factor alpha (TNF-α), interleukin 17A (IL17A) and inducible of nitric oxide synthase (iNOs), and p65 protein phosphorylation (NF-κB) were analysed 4 h after pleurisy induction. Animals pre-treated with CE, BuOH, Aq, SA, or LA inhibited leukocytes, exudation, MPO and ADA activities, NO x , IL-1β, TNF-α, and IL-17A levels, and the mRNA expression for IL-1β, TNF-α, IL-17A, iNOS, and p65 protein phosphorylation (NF-κB) (p < 0.05). Our study demonstrated that J. sellowii can protect against inflammation induced by Cg by decreasing the leukocytes and exudation. Its effects are related to the decrease of either proinflammatory cytokines and/or NO x . The isolated compounds SA and LA may play an important role in this anti-inflammatory action by inhibiting all the studied parameters. The anti-inflammatory properties of these compounds are due to the downregulation of NF-κB.

Three new trixane glycosides obtained from the leaves of Jungia sellowii Less. using centrifugal partition chromatography

“…Pharmacological studies demonstrated that the anti-inflammatory and antioxidant effects of J. paniculata were associated to the presence of flavonoids and other polyphenols [ 4 ]. Recently, we reported the in vivo anti-inflammatory properties of an aqueous fraction of the leaves of J. sellowii , that is in agreement with its popular use in Brazil [ 5 ].…”

Phenolic Compounds Isolated fromCalea unifloraLess. Promote Anti-Inflammatory and Antioxidant Effects in Mice Neutrophils (Ex Vivo) and in Mice Pleurisy Model (In Vivo)

“…In this study, these cytokine levels were reduced by NRG and AH-BU, which are consistent with the results of another study conducted by Nader et al [38]. This research group demonstrated that Jungia sellowii , a species also belonging to the Asteraceae family, reduced IL-1 β and IL-17A levels as well as neutrophil infiltration in a murine model of carrageenan-induced lung inflammation [38].…”

Sodium fluoride induces renal inflammatory responses by activating NF-κB signaling pathway and reducing anti-inflammatory cytokine expression in mice

“…Up-regulation of iNOS occurs mostly in association with the inflammation and infection as a part of defensive reaction [ 36 ]. Meanwhile, iNOS can catalyze the production of NO, and NO is known to participate in the inflammation responses by increasing the inflammatory cell infiltration and vascular permeability [ 20 , 45 , 46 ]. In the present study, we found that the NO contents as well as the iNOS activities and mRNA expression levels were significantly increased in the 24 and 48 mg/kg groups, suggesting that NaF exposure induced the production of NO and iNOS, and that the up-regulation in the transcriptional expression of iNOS was the possible reason of the increase in NO generation.…”