Jun Gene Expression is Decreased in Parathyroid Adenoma

Abstract

2Kierownik: dr hab. J. GawrychowskiThe aim of the study was to analyze the gene expression of JUn and ccnd1 in a group of parathyroid tissues obtained from patients with primary hyperparathyroidism in comparison to hyperplastic parathyroid and normal/atrophic parathyroid tissues by real-time quantitative PCR. Our goal was to validate the conclusion of Forsberg et al (2005) who reported overexpression of JUn in parathyroid adenomas by a joint microarray and QPCR study. material and methods. The analysis of JUn, ccnd1 was carried out by QPCR in 14 parathyroid adenomas, 8 hyperplasia cases and 50 normal/atrophic parathyroid samples taken intraoperatively. Expression of the examined genes was normalized to the reference index (geometric mean of reference genes expression: eiF3s10, UBe2d2, atP6V1e). Results. We observed a decrease of JUn expression in parathyroid adenomas in comparison to both normal/atrophic and hyperplastic parathyroids. The fold change value was 0.71 in comparison of adenomas to normal/atrophic samples (p = 0.044) and 0.75 to hyperplastic glands (p = 0.003). For ccnd1 we observed one case of parathyroid adenoma with a very clearly increased expression, while 3 adenomas (21.4% of all adenomas) exhibited the increase over the highest value seen in normal parathyroids (fold change = 3.52). conclusions. In parathyroid adenomas we were not able to confirm any overexpression of JUn gene, as suggested by the previous study. On the contrary, we observed a distinct inhibition of JUn RNA expression in comparison to non-neoplastic parathyroids. For ccnd1 gene overexpression in parathyroid adenomas, we report the frequency of 21.4%.

Simultaneous expression analysis of vitamin D receptor, calcium-sensing receptor, cyclin D1, and PTH in symptomatic primary hyperparathyroidism in Asian Indians

“…We found overexpression of CD1 mRNA in 85% of the parathyroid adenomas in comparison to 20-40% reported previously (19,20,21,22,23). Of the four genes (VDR, CASR, CD1, and PTH) analyzed in this study, only CD1 expression was significantly associated with parathyroid adenoma weight.…”

“…In contrast, previous studies have observed both nuclear, and to a lesser degree, cytoplasmic staining (19,20,21,22,23). Overexpression of CD1 both in nuclear and in cytoplasmic compartments has been reported in many human malignancies.…”

“…Abnormalities in VDR (12,13,14), CASR (14,15,16,17,18), and CD1 (19,20,21,22,23) expression have been reported in the cases probably having mild form of PHPT, parathyroid cancers, and renal secondary hyperparathyroidism. However, very little is known about such abnormalities in adenomas from patients with symptomatic sporadic PHPT (24,25,26).…”