Joint Positive Control Testing in Guinea Pig Skin Sensitization Tests

Wiemann, Christiane; Berthold, K.; Heusener, A.; Krüger, Natalia; Seeberger, A.; Stropp, G.

doi:10.1006/rtph.2001.1503

Cited by 5 publications

(2 citation statements)

References 19 publications

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“…The Buehler assay is suitable for testing formulated products, as the method involves the topical application of the test substances (Botham et al, 2005); the guinea pig is used as the standard animal for skin sensitization testing (Wiemann et al, 2002). In general, a score of 1 or higher on the Magnusson and Kligman scale is regarded as a positive response, provided that the score value in the control animals is less than 1 (ISO, 2002).…”

Section: Discussionmentioning

confidence: 99%

Dermal Toxicity of a Repellent Formulation Containing <i>Piper Aduncum</i> Linnaeus (Piperales: Piperaceae) Essential Oil

Mamood¹,

Othman²,

Nasir³

et al. 2017

American Journal of Pharmacology and Toxicology

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Abstract:It has been shown in previous research that Piper aduncum Linnaeus essential oil has the potential to be developed as an alternative mosquito repellent. When the essential oil was formulated into cream, it was able to provide >2h of protection against Aedes aegypti in the laboratory; thus, it can be commercialized as an alternative to synthetic repellent especially N, N-diethyl-3-methylbenzamide (DEET). In this study, the irritation and sensitization potential of a cream formulation containing P. aduncum essential oil was investigated to verify its safety for application purposes. The P. aduncum essential oil was formulated into a cream containing 10% of the essential oil for irritation and skin sensitization assays on New Zealand white rabbits and guinea pigs (Hartley strain), respectively, following the ISO10993-10:2010 (E) guidelines. The macroscopic and histological observations from both assays revealed that the cream formulation containing P. aduncum essential oil caused slight irritation on rabbit skin, with a Primary Irritation Index (PII) of 1.54; however, no positive response was detected in the skin sensitization assay. In conclusion, the cream formulation containing 10% P. aduncum essential oil was slightly irritating to rabbit skin but did not cause sensitization in the animals tested.

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Section: Discussionmentioning

confidence: 99%

Dermal Toxicity of a Repellent Formulation Containing <i>Piper Aduncum</i> Linnaeus (Piperales: Piperaceae) Essential Oil

Mamood¹,

Othman²,

Nasir³

et al. 2017

American Journal of Pharmacology and Toxicology

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“…자외선에 지속적으로 노출되다 보면 홍반증상에서부터 시작해서 심하게는 유전자의 손상이 발생해 피부암을 유발하게 되므로 광에 의 해 화장품이 영향을 받을 가능성이 있는지 확인해야 한다 (Grosjean & Sarasin, 2005). 따라서 광감작성 및 광독성 시험을 진행해야 하며 설치 류 등에는 피부암이 유발될 수 있고 일반적으로 토끼, 기니픽 등의 동물 로 실험한다 (Wiemann et al, 2001) Table 10. Mortalities for 15 days in rats administered with 300 mg/kg (G1 and G2) or 2,000 mg/kg (G3 and G4) of the Padina gymnospora extract…”

Section: 광감작성 시험: Photosensitivity Test (Harber Test)unclassified

Safety Assessment of Ethanolic Extract of Padina gymnospora as a Cosmetic Ingredient

Kang¹,

Bak²,

Kim³

et al. 2021

Asian J Beauty Cosmetol

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Purpose: In a previous study, we identified the skin-whitening effect of the ethanolic extract of Padina gymnospora. The present study was performed to confirm the safety of the extract in animal replacement tests.Methods: To evaluate the safety of the extract of Padina gymnospora, the photosensitivity test (Harber test), in vitro 3T3 neutral red uptake (3T3 NRU) phototoxicity test, local lymph node assay (5-bromo-2'-deoxyuridine enzyme-linked immunosorbent assay), acute oral toxicity test, and reconstructed human epidermis (RHE) test were used. All experiments followed the guidelines of the Korean Ministry of Food and Drug Safety and were conducted by a GLP-certified organization (Chemon Inc.).Results: The extract of Padina gymnospora was not photosensitive: 0% photosensitization was detected (I grade: very weak). In the 3T3 NRU phototoxicity test, the relative viability of the extract-treated cells was higher than the guideline level; thus, the extract was classified as non-phototoxic. Treatment with the extract did not trigger skin irritation in the RHE test model and did not cause skin sensitization in the local lymph node assay. Finally, oral administration of the extract to rats indicated that it was not a harmful material as the LD50 was estimated at >2,000 mg/kg.Conclusion: The ethanolic extract of Padina gymnospora was demonstrated to be safe when applied to the skin. Taken together with our previous study of its efficacy, we conclude that this extract has the potential for use as a cosmetic ingredient.

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A comparative study of the sensitivity of the 3-induction and 9-induction Buehler test procedures for assessing skin sensitisation potential

Botham

Urtizberea

Wiemann³

et al. 2005

Food and Chemical Toxicology

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Joint Positive Control Testing in Guinea Pig Skin Sensitization Tests

Cited by 5 publications

References 19 publications

Dermal Toxicity of a Repellent Formulation Containing <i>Piper Aduncum</i> Linnaeus (Piperales: Piperaceae) Essential Oil

Dermal Toxicity of a Repellent Formulation Containing <i>Piper Aduncum</i> Linnaeus (Piperales: Piperaceae) Essential Oil

Safety Assessment of Ethanolic Extract of Padina gymnospora as a Cosmetic Ingredient

A comparative study of the sensitivity of the 3-induction and 9-induction Buehler test procedures for assessing skin sensitisation potential

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