A comparative study of the sensitivity of the 3-induction and 9-induction Buehler test procedures for assessing skin sensitisation potential

Botham, Philip A.; Urtizberea, M.; Wiemann, Christiane; Manciaux, X.; Tilbury, Lorraine; Vohr, Hans-Werner; Allen, Stephanie; Carmichael, Neil G.; Jouffrey, S. de

doi:10.1016/j.fct.2004.08.013

Cited by 6 publications

(2 citation statements)

References 11 publications

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“…It is clear that phthalic anhydride elicits positive responses in the LLNA , the Buehler test (Botham et al 2005) and the GPMT (Cronin & Basketter 1994;Gerberick et al 2000). It is clear that phthalic anhydride elicits positive responses in the LLNA , the Buehler test (Botham et al 2005) and the GPMT (Cronin & Basketter 1994;Gerberick et al 2000).…”

Section: Behavior Of Phthalic Anhydride In Tests For Skin Sensitizationmentioning

confidence: 99%

Phthalic anhydride: Illustrating a conundrum in chemical allergy

Basketter

Kimber

2016

Journal of Immunotoxicology

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Although a substantial number of chemicals has the ability to bind covalently to proteins and thereby, given sufficient exposure, induce a state of sensitization, only a small minority appear to be able to cause allergic hypersensitivity of the respiratory tract; the great majority being exclusively skin sensitizers. The key mechanistic drivers for the differentiation between skin and respiratory sensitization are already well characterized at the cellular/cytokine level. However, at both the chemical level and in terms of predictive toxicology, matters are much less clear. In the present article, phthalic anhydride is used as an exemplar, since it displays a particularly differentiated profile as a chemical allergen. Whereas most respiratory sensitizers are known also to give rise to delayed skin reactions, evidence for phthalic anhydride suggests that it only causes immediate type allergy. Chemically, phthalic anhydride can be presumed to react similar to other respiratory sensitizing anhydrides; in predictive tests for skin sensitization, phthalic anhydride is clearly positive, a property it has in common with all other chemical respiratory allergens. Thus, in the context of interpreting predictive toxicology tests for skin sensitization, the inference is that negative results demonstrate an absence of both skin- and respiratory-sensitizing capacity.

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Section: Behavior Of Phthalic Anhydride In Tests For Skin Sensitizationmentioning

confidence: 99%

Phthalic anhydride: Illustrating a conundrum in chemical allergy

Basketter

Kimber

2016

Journal of Immunotoxicology

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“…The Buehler assay is suitable for testing formulated products, as the method involves the topical application of the test substances (Botham et al, 2005); the guinea pig is used as the standard animal for skin sensitization testing (Wiemann et al, 2002). In general, a score of 1 or higher on the Magnusson and Kligman scale is regarded as a positive response, provided that the score value in the control animals is less than 1 (ISO, 2002).…”

Section: Discussionmentioning

confidence: 99%

Dermal Toxicity of a Repellent Formulation Containing <i>Piper Aduncum</i> Linnaeus (Piperales: Piperaceae) Essential Oil

Mamood¹,

Othman²,

Nasir³

et al. 2017

American Journal of Pharmacology and Toxicology

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Abstract:It has been shown in previous research that Piper aduncum Linnaeus essential oil has the potential to be developed as an alternative mosquito repellent. When the essential oil was formulated into cream, it was able to provide >2h of protection against Aedes aegypti in the laboratory; thus, it can be commercialized as an alternative to synthetic repellent especially N, N-diethyl-3-methylbenzamide (DEET). In this study, the irritation and sensitization potential of a cream formulation containing P. aduncum essential oil was investigated to verify its safety for application purposes. The P. aduncum essential oil was formulated into a cream containing 10% of the essential oil for irritation and skin sensitization assays on New Zealand white rabbits and guinea pigs (Hartley strain), respectively, following the ISO10993-10:2010 (E) guidelines. The macroscopic and histological observations from both assays revealed that the cream formulation containing P. aduncum essential oil caused slight irritation on rabbit skin, with a Primary Irritation Index (PII) of 1.54; however, no positive response was detected in the skin sensitization assay. In conclusion, the cream formulation containing 10% P. aduncum essential oil was slightly irritating to rabbit skin but did not cause sensitization in the animals tested.

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Formaldehyd [MAK Value Documentation in German language, 2010]

2012

The MAK‐Collection for Occupational Health and Safety

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A comparative study of the sensitivity of the 3-induction and 9-induction Buehler test procedures for assessing skin sensitisation potential

Cited by 6 publications

References 11 publications

Phthalic anhydride: Illustrating a conundrum in chemical allergy

Phthalic anhydride: Illustrating a conundrum in chemical allergy

Dermal Toxicity of a Repellent Formulation Containing <i>Piper Aduncum</i> Linnaeus (Piperales: Piperaceae) Essential Oil

Formaldehyd [MAK Value Documentation in German language, 2010]

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