Joint associations of folate, homocysteine and MTHFR, MTR and MTRR gene polymorphisms with dyslipidemia in a Chinese hypertensive population: a cross-sectional study

Li, Wen‐Xing; Lv, Wen; Dai, Shao‐Xing; Pan, Mingluo; Huang, Jing‐Fei

doi:10.1186/s12944-015-0099-x

Cited by 36 publications

(43 citation statements)

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“…This study was conducted using data collected in a previous study (16). This was a multicenter, randomized, double-blind controlled trial in hypertensive Chinese adults.…”

Section: Methodsmentioning

confidence: 99%

“…This was a multicenter, randomized, double-blind controlled trial in hypertensive Chinese adults. Details regarding "Study subjects," "Randomization and double blinding," "Data collection procedures," and "Laboratory tests" have been previously described (16,17). In total, 480 patients with mild or moderate hypertension were recruited from six hospitals in different Chinese regions (Ha'rbin, Shanghai, Shenyang, Beijing, Xi'an, and Nanjing) from September to December 2005.…”

Section: Methodsmentioning

confidence: 99%

“…Hcy concentration was determined in duplicate by high-performance liquid chromatography. The intra-and inter-assay coefficients of variation were 3.5% and 4.2%, respectively (16). Serum folate was determined by chemiluminescent immunoassay using a Beckman Coulter ACCESS Immunoassay System (Beckman-Coulter Canada Inc., Mississauga, Canada).…”

Section: Methodsmentioning

confidence: 99%

“…Serum folate was determined by chemiluminescent immunoassay using a Beckman Coulter ACCESS Immunoassay System (Beckman-Coulter Canada Inc., Mississauga, Canada). The intra-and inter-assay coefficients of variation were 2.3% and 3.7%, respectively (16). The polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method was applied to detect the MTHFR C677T, MTHFR A1298C, MTR A2756G, and MTRR A66G genotypes.…”

Section: Methodsmentioning

confidence: 99%

“…Further, the combination effects of genotypes and low folate increased the dyslipidemia risk (16). Based on the metabolism connections between folate and these hepatic enzymes, we hypothesized that these four gene polymorphisms in combination with Hcy/folate may affect the hepatic functions.…”

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confidence: 99%

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Folate Deficiency Was Associated with Increased Alanine Aminotransferase and Glutamyl Transpeptidase Concentrations in a Chinese Hypertensive Population: A Cross-Sectional Study

Wei

Cao

et al. 2016

J Nutr Sci Vitaminol

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Summary Alanine aminotransferase (ALT), aspartate transaminase (AST), and glutamyl transpeptidase (GGT) were three key enzymes in the hepatic metabolism. This study aimed to investigate the effect of homocysteine (Hcy) metabolism gene polymorphisms and serum Hcy and folate level on the hepatic functions in a Chinese hypertensive population. A representative sample with 480 subjects aged 28-75 was enrolled in 2005.9-2005.12 from six hospitals in different Chinese regions. Serum ALT, AST and GGT were measured by using an automatic biochemistry analyzer. Serum Hcy was measured by high-performance liquid chromatography, and serum folate was measured by chemiluminescent immunoassay. Known genotypes were detected by PCR-RFLP methods. The results showed that the MTHFR C677T mutation was related a decreased serum AST level (r520.11, p50.026), whereas the MTHFR A1298C mutation elevated serum AST level (r50.11, p50.032). Furthermore, multiple regression analysis showed that folate deficiency was associated with higher serum ALT (b (SE): 0.13 (0.06), p50.031) and GGT level (b (SE): 0.18 (0.07), p50.011). However, serum Hcy level may not affect the hepatic functions. Our data suggested that hepatic functions were affected by MTHFR gene polymorphisms and serum folate level. Further studies are needed to confirm these correlations in a larger population. Key Words folate deficiency, MTHFR gene polymorphisms, alanine aminotransferase, aspartate transaminase, glutamyl transpeptidase Fatty liver disease (FLD), including primary non-alcoholic fatty liver disease (NAFLD) and the severe form, non-alcoholic steatohepatitis (NASH) is the most common form of liver disease and is increasing throughout the world (1). In Western adults, the prevalence of NAFLD assessed by ultrasound is 20-30% and the prevalence of NASH is 3-16% (2). Alanine aminotransferase (ALT), aspartate transaminase (AST) and glutamyl transpeptidase (GGT) are three key enzymes in the liver metabolism. Studies showed that high levels of ALT, AST and GGT were associated with liver disease (3). Furthermore, serum ALT and AST levels showed relevance with the histopathological changes in liver biopsies of hepatitis C patients (4). In one carbon metabolism, folate is closely related to many important biochemical processes in vivo. A mouse experiment suggested that maternal low folate and selenium diet altered liver gene expression patterns in the offspring after weaning (5). And a previous study showed that folate supplementation reduced the serum ALT level in high baseline ALT (.40 IU/L) individuals (6). In addition, a higher serum homocysteine (Hcy) level also showed strongly correlation with liver disease (7,8).Methylenetetrahydrofolate reductase (MTHFR), methionine synthase (MTR) and methionine synthase reductase (MTRR) are three key regulatory enzymes in the folate and Hcy metabolisms (9). MTHFR catalyses the reduction of 5,10-methylenetetrahydrofolate (5,10-MTHF) to 5-methyltetrahydrofolate (5-MTHF) and 5-MTHF is a cofactor for Hcy methylation to methionine when cata...

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“…This study was conducted using data collected in a previous study (16). This was a multicenter, randomized, double-blind controlled trial in hypertensive Chinese adults.…”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

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