Jinshanibacter, a new genus of Budviciaceae: identification of Jinshanibacter zhutongyuii sp. nov. and Jinshanibacter xujianqingii sp. nov. isolated from cloacal content of snow finch (Montifringilla taczanowskii)

Abstract

Four novel strains isolated from the cloacal contents of snow finches (Montifringilla taczanowskii) were characterized as aerobic, Gram-stain-negative, slightly motile, and rod-shaped. Analysis of the 16S rRNA gene sequence revealed that strain CF-458T had the highest similarities of 96.9 and 96.4 % with Limnobaculum parvum HYN0051T and Pragia fontium DSM 5563T, while strain CF-1111T shared the highest similarities of 96.4 and 96.1 % with Pantoea rodasii LMG 26273T and Pectobacterium punjabense SS95T. Phylogenomic analysis showed the four isolates were separated into group Ⅰ (CF-458T and CF-917) and group Ⅱ (CF-1111T and CF-509), and clustered independently in the vicinity of the genera Limnobaculum and Pragia . Summed feature 3 (C16 : 1 ω7c and/or C16 : 1 ω6c, 23.9 and 17.2 %, respectively), C16 : 0 (21.8 and 22.1 %, respectively) and C14 : 0 (10.6 and 17.7 %, respectively) were the common major fatty acids, and summed feature 8 (C18 : 1 ω7c and/or C18 : 1 ω6c, 12.3 %) was also a major fatty acid for strain CF-458T while cyclo-C17 : 0 (13.1%) was for strain CF-1111T. Both had Q-8 as the sole quinone and contained phosphatidylethanolamine, phosphatidylglycerol, and diphosphatidylglycerol as the major polar lipids. The DNA G+C content of strains CF-458T and CF-1111T was 45.7 and 45.4 mol%, respectively. Based on taxonomic position in the phylogenomic tree and phenotypic properties, two novel species of a new genus within the family Budviciaceae are thus proposed, with the name Jinshanibacter gen. nov., zhutongyuii sp. nov. (type strain CF-458T=CGMCC 1.16483T=GDMCC 1.1586T=JCM 33489T) and Jinshanibacter xujianqingii sp. nov. (type strain CF-1111T=CGMCC 1.16786T=GDMCC 1.1587T=JCM 33490T), respectively.

Jinshanibacter allomyrinae sp. nov., isolated from larvae of Allomyrina dichotoma, proposal of Insectihabitans xujianqingii gen. nov., comb. nov. and emended descriptions of the genera Jinshanibacter, Limnobaculum and Pragia

“…The former strain also did not grow at pH 10.0. All the above results were different from those previously reported [8], which might be caused by erroneous reading. On the other hand, P. fontium DSM 5563 T did not produce acid from d-galactose and d-xylose, whereas L. parvum KACC 19816 T showed positive response in activities of N-acetyl-β-glucosamidase, acid phosphatase, esterase (C4), leucine arylamidase and naphthol-AS-BI-phosphohydrolase, respectively, in contrast previously reported results [5,6].…”

“…Cells of the isolates were found to be non-motile. Members of the genus Jinshanibacter were recently described to be slightly motile based on motility test on semisolid agar [8] but they were shown to be non-motile in our study. The absence of flagella in the isolates and members of the genera Jinshanibacter and Limnobaculum was also confirmed in that most of the genes involved in flagellar biosynthesis and assembly were absent in their genomes, whereas P. fontium DSM 5563 T , which is known to be motile by means of peritrichous flagella [5], contained all the genes involved in flagellar biosynthesis and assembly (Table S1).…”

“…xujianqingii JCM 33490 T and J. zhutongyuii JCM 33489 T contained Q-8 as the only respiratory quinone, as previously reported [8]. The cellular fatty acid profiles of the four isolates and their phylogenetic relatives are given in .…”

“…3c, d), in contrast to the polar lipid profiles previously reported [8]. The mobility of the spot identified as DPG by Ge et al [8] was different from that of authentic DPG (Sigma). Moreover, L. parvum and P. fontium was reported to have polar lipid profiles of PE, PG and PL [6] but they also contained an APL like the isolates and two Jinshanibacter species in our study (Fig.…”

“…Members of the family have been isolated from gut of milipede, clinical samples and well or lake water. The fifth member of the family, Jinshanibacter , was recently proposed by Ge et al [8] with description of two species to encompass the strains isolated from cloacal contents of snow finches.…”