Jiangella gansuensis gen. nov., sp. nov., a novel actinomycete from a desert soil in north-west China

Abstract

A novel actinomycete strain, designated YIM 002T, was isolated from a desert soil sample in Gansu Province, north-west China. This actinomycete isolate formed well-differentiated aerial and substrate mycelia. In the early stages of growth, the substrate mycelia fragmented into short or elongated rods. Chemotaxonomically, it contained ll-2,6-diaminopimelic acid in the cell wall. The cell-wall sugars contained ribose and glucose. Phospholipids present were phosphatidylinositol mannosides, phosphatidylinositol and diphosphatidylglycerol. MK-9(H4) was the predominant menaquinone. The major fatty acids were anteiso C15 : 0 (35·92 %), anteiso C17 : 0 (15·84 %), iso C15 : 0 (10·40 %), iso C16 : 0 (7·07 %) and C17 : 1 ω8c (9·37 %). The G+C content of the DNA was 70 mol%. Phylogenetic analysis and signature nucleotide data based on 16S rRNA gene sequences showed that strain YIM 002T is distinct from all recognized genera of the family Nocardioidaceae in the suborder Propionibacterineae. On the basis of the phenotypic and genotypic characteristics, it is proposed that isolate YIM 002T be classified as a novel species in a new genus, Jiangella gansuensis gen. nov., sp. nov. The type strain is YIM 002T (=DSM 44835T=CCTCC AA 204001T=KCTC 19044T).

Jiangella alkaliphila sp. nov., an actinobacterium isolated from a cave

“…On the basis of the phenotypic and DNA-DNA hybridization data presented, strain D8-87 T is considered to represent a novel species of the genus Jiangella, for which the name Jiangella alkaliphila is proposed. The type strain is D8-87 T (5JBRI 2008 T 5KCTC 19222 T 5DSM 45079 T ).The genus Jiangella was proposed by Song et al (2005) to accommodate an actinomycete isolated from a desert soil in north-west China. At the time of writing, Jiangella gansuensis remains the type and only species of the genus.…”

“…Chemotaxonomically, the genus is characterized by the presence of LL-2,6-diaminopimelic acid (LL-DAP) in the cell-wall peptidoglycan, MK-9(H 4 ) as the major menaquinone, anteiso-C 15 : 0 , anteiso-C 17 : 0 , iso-C 15 : 0 , iso-C 16 : 0 and C 17 : 1 v8c as major fatty acids and a DNA G+C content of 70 mol%. 16S rRNA gene sequence analyses (Song et al, 2005) indicated that the genus belongs to the family Nocardioidaceae Nesterenko et al 1985 within the suborder Propionibacterineae.During the course of studies on the diversity of cave bacteria, a mycelium-forming actinomycete, designated strain D8-87 T , was isolated from the soil of a natural cave in Jeju, Republic of Korea, and its taxonomic status was investigated by a polyphasic approach. Strain D8-87 T was isolated by a dilution plating method on starch-casein agar as the selective medium.…”

“…Chemotaxonomically, the genus is characterized by the presence of LL-2,6-diaminopimelic acid (LL-DAP) in the cell-wall peptidoglycan, MK-9(H 4 ) as the major menaquinone, anteiso-C 15 : 0 , anteiso-C 17 : 0 , iso-C 15 : 0 , iso-C 16 : 0 and C 17 : 1 v8c as major fatty acids and a DNA G+C content of 70 mol%. 16S rRNA gene sequence analyses (Song et al, 2005) indicated that the genus belongs to the family Nocardioidaceae Nesterenko et al 1985 within the suborder Propionibacterineae.…”

Jiangella alba sp. nov., an endophytic actinomycete isolated from the stem of Maytenus austroyunnanensis

“…At present, this genus comprises only two species, Jiangella gansuensis (Song et al, 2005) and Jiangella alkaliphila (Lee, 2008), which were both isolated from soil samples. In this paper, we describe the polyphasic characterization of strain YIM 61503 T isolated from an endophytic environment.…”

“…The genus Jiangella was proposed by Song et al (2005) to accommodate an aerobic, Gram-positive actinomycete with LL-2,6-diaminopimelic acid (LL-DAP) in the cell-wall peptidoglycan and MK-9(H 4 ) as the predominant menaquinone. At present, this genus comprises only two species, Jiangella gansuensis (Song et al, 2005) and Jiangella alkaliphila (Lee, 2008), which were both isolated from soil samples.…”