This chapter deals with a learning scenario that has been developed for the acquisition of e-business competencies in various organizations. A constructivist cross-teaching-approach as well as the results of the empirical research derived from of a virtually realized interregional cooperation between a German and an Austrian university concerning business sciences from winter term 2010/11 and summer terms 2012 and 2013 are presented. A teaching-learning situation was created where learners can collaborate in virtual communities that come close to the reality of globalized business. The students worked out an e-business case study in interregional learning groups. The particular interest within the evaluation of this study lies in the virtual collaboration of the interregional study groups and the communication media used but also in the effect the intercultural didactic scenario has on heterogeneous study groups.