Jasmonic acid enhances osmotic stress responses by <scp>MYC2</scp>‐mediated inhibition of <i>protein phosphatase 2C1</i> and <i>response regulators 26</i> transcription factor in tomato

Zhao, Wenchao; Huang, Huang; Wang, Jingjing; Wang, Xiaoyun; Xu, Bingqin; Yao, Xuehui; Sun, Lulu; Yang, Rui; Sun, Aidong; Wang, Shaohui

doi:10.1111/tpj.16067

Cited by 20 publications

(8 citation statements)

References 75 publications

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“…JA signaling also plays important roles to regulate drought response independently or through cooperating with ABA [52][53][54]. Drought treatment induced JA and ABA accumulation in maize with earlier induction for JA production [55].…”

Section: Discussionmentioning

confidence: 99%

Maize Transcription Factor ZmHsf28 Positively Regulates Plant Drought Tolerance

Liu

Zhang

Tang

et al. 2023

IJMS

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Identification of central genes governing plant drought tolerance is fundamental to molecular breeding and crop improvement. Here, maize transcription factor ZmHsf28 is identified as a positive regulator of plant drought responses. ZmHsf28 exhibited inducible gene expression in response to drought and other abiotic stresses. Overexpression of ZmHsf28 diminished drought effects in Arabidopsis and maize. Gene silencing of ZmHsf28 via the technology of virus-induced gene silencing (VIGS) impaired maize drought tolerance. Overexpression of ZmHsf28 increased jasmonate (JA) and abscisic acid (ABA) production in transgenic maize and Arabidopsis by more than two times compared to wild-type plants under drought conditions, while it decreased reactive oxygen species (ROS) accumulation and elevated stomatal sensitivity significantly. Transcriptomic analysis revealed extensive gene regulation by ZmHsf28 with upregulation of JA and ABA biosynthesis genes, ROS scavenging genes, and other drought related genes. ABA treatment promoted ZmHsf28 regulation of downstream target genes. Specifically, electrophoretic mobility shift assays (EMSA) and yeast one-hybrid (Y1H) assay indicated that ZmHsf28 directly bound to the target gene promoters to regulate their gene expression. Taken together, our work provided new and solid evidence that ZmHsf28 improves drought tolerance both in the monocot maize and the dicot Arabidopsis through the implication of JA and ABA signaling and other signaling pathways, shedding light on molecular breeding for drought tolerance in maize and other crops.

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Section: Discussionmentioning

confidence: 99%

Maize Transcription Factor ZmHsf28 Positively Regulates Plant Drought Tolerance

Liu

Zhang

Tang

et al. 2023

IJMS

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“…The crosstalk between JA and ABA has been extensively implicated. AtMYC2 acts as a transcriptional activator in abscisic acid signaling (Abe et al ., 2003), and it negatively regulates the ABA signaling negative regulator, SlPP2C1, in tomato (W. C. Zhao et al ., 2023). ABA inducibility of both ANAC019 and ANAC055 is diminished in the myc2 mutant (Jiang et al ., 2009), indicating that MYC2 serves as a central connection between JA and ABA signaling.…”

Section: Discussionmentioning

confidence: 99%

The AP2/ERF transcription factor PtoERF15 confers drought tolerance via JA‐mediated signaling in Populus

Kong,

Song,

Wei

et al. 2023

New Phytologist

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Summary Drought stress is one of the major limiting factors for the growth and development of perennial trees. Xylem vessels act as the center of water conduction in woody species, but the underlying mechanism of its development and morphogenesis under water‐deficient conditions remains elucidation. Here, we identified and characterized an osmotic stress‐induced ETHYLENE RESPONSE FACTOR 15 (PtoERF15) and its target, PtoMYC2b, which was involved in mediating vessel size, density, and cell wall thickness in response to drought in Populus tomentosa. PtoERF15 is preferentially expressed in differentiating xylem of poplar stems. Overexpression of PtoERF15 contributed to stem water potential maintaining, thus promoting drought tolerance. RNA‐Seq and biochemical analysis further revealed that PtoERF15 directly regulated PtoMYC2b, encoding a switch of JA signaling pathway. Additionally, our findings verify that three sets of homologous genes from NAC (NAM, ATAF1/2, and CUC2) gene family: PtoSND1‐A1/A2, PtoVND7‐1/7‐2, and PtoNAC118/120, as the targets of PtoMYC2b, are involved in the regulation of vessel morphology in poplar. Collectively, our study provides molecular evidence for the involvement of the PtoERF15‐PtoMYC2b transcription cascade in maintaining stem water potential through the regulation of xylem vessel development, ultimately improving drought tolerance in poplar.

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“…POD, SOD, MDA, PRO, and H 2 O 2 activities were determined using corresponding assay kits (Keming Bioengineering Institute). The H 2 O 2 production in guard cells was analyzed by using 50 μM H 2 DCFDA (Coolaber) as described previously [ 67 , 68 ]. Leaves were incubated (20 min in darkness) in a staining buffer (loading buffer containing 50 μM H 2 DCFDA and 10 mM MES–Tris, pH 6.15), and washed five times in phosphate-buffered saline to remove excess H 2 DCFDA.…”

Section: Methodsmentioning

confidence: 99%

RtNAC055 promotes drought tolerance via a stomatal closure pathway linked to methyl jasmonate/hydrogen peroxide signaling in Reaumuria trigyna

Ma,

Zhang,

Guo

et al. 2024

Horticulture Research

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The stomata regulate CO2 uptake and efficient water usage, thereby promoting drought stress tolerance. NAC proteins (NAM, ATAF1 / 2, and CUC2) participate in plant reactions following drought stress, but molecular mechanism underlying NAC-mediated regulation of stomatal movement are unclear. In this study, a novel NAC gene from Reaumuria trigyna, RtNAC055, was found to enhance drought tolerance via a stomatal closure pathway. It was regulated by RtMYC2 and integrated with jasmonic acid signaling and was predominantly expressed in stomata and root. The suppression of RtNAC055 could improve JA and H2O2 production and increase the drought tolerance of transgenic R. trigyna callus. Ectopic expression of RtNAC055 in Arabidopsis atnac055 mutant rescued their drought-sensitive phenotype by decreasing stomatal aperture. Under drought stress, overexpression of RtNAC055 in poplar promoted ROS(H2O2) accumulation in stomata, which accelerated stomatal closure and maintained a high photosynthetic rate. Drought upregulated the expression of PtRbohD / F, PtP5CS2, and PtDREB1.1, as well as antioxidant enzyme activities in heterologous expression poplars. RtNAC055 promoted H2O2 production in guard cells by directly binding to the promoter of RtRbohE, thus regulating stomatal closure. The stress-related genes RtDREB1.1 / P5CS1 were directly regulated by RtNAC055. These results indicate that RtNAC055 regulates stomatal closure by maintaining the balance between the antioxidant system and H2O2 level, reducing the transpiration rate and water loss, and improving photosynthetic efficiency and drought resistance.

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Jasmonic acid enhances osmotic stress responses by MYC2‐mediated inhibition of protein phosphatase 2C1 and response regulators 26 transcription factor in tomato

Cited by 20 publications

References 75 publications

Maize Transcription Factor ZmHsf28 Positively Regulates Plant Drought Tolerance

Maize Transcription Factor ZmHsf28 Positively Regulates Plant Drought Tolerance

The AP2/ERF transcription factor PtoERF15 confers drought tolerance via JA‐mediated signaling in Populus

RtNAC055 promotes drought tolerance via a stomatal closure pathway linked to methyl jasmonate/hydrogen peroxide signaling in Reaumuria trigyna

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