Maize Transcription Factor ZmHsf28 Positively Regulates Plant Drought Tolerance

Liu, Lijun; Zhang, Yuhan; Tang, Chen; Shen, Qinqin; Fu, Jingye; Wang, Qiang

doi:10.3390/ijms24098079

Cited by 4 publications

(4 citation statements)

References 88 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…RNA extraction from maize protoplasts was performed as described previously [ 64 ]. The HiPure HP Plant RNA Mini Kit (Magen Biotech, Guangzhou, China) was used to extract RNA from maize protoplasts following the manufacture protocol.…”

Section: Methodsmentioning

confidence: 99%

“…cDNA was prepared using the total RNA extracted above with the reverse transcriptase kit (Vazyme Biotech, Nanjing, China). Quantitative PCR (qPCR) analysis was conducted using the SYBR GREEN qPCR Master MIX (Vazyme Biotech) on the Bio-Rad CFX96 as described previously [ 64 ]. The maize gene ZmEf1a was used as the endogenous control according to previous study [ 64 ].…”

Section: Methodsmentioning

confidence: 99%

“…Quantitative PCR (qPCR) analysis was conducted using the SYBR GREEN qPCR Master MIX (Vazyme Biotech) on the Bio-Rad CFX96 as described previously [ 64 ]. The maize gene ZmEf1a was used as the endogenous control according to previous study [ 64 ].…”

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Genome-wide identification of ZmMYC2 binding sites and target genes in maize

Liu,

Zhang,

Tang

et al. 2024

BMC Genomics

Self Cite

View full text Add to dashboard Cite

Background Jasmonate (JA) is the important phytohormone to regulate plant growth and adaption to stress signals. MYC2, an bHLH transcription factor, is the master regulator of JA signaling. Although MYC2 in maize has been identified, its function remains to be clarified. Results To understand the function and regulatory mechanism of MYC2 in maize, the joint analysis of DAP-seq and RNA-seq is conducted to identify the binding sites and target genes of ZmMYC2. A total of 3183 genes are detected both in DAP-seq and RNA-seq data, potentially as the directly regulating genes of ZmMYC2. These genes are involved in various biological processes including plant growth and stress response. Besides the classic cis-elements like the G-box and E-box that are bound by MYC2, some new motifs are also revealed to be recognized by ZmMYC2, such as nGCATGCAnn, AAAAAAAA, CACGTGCGTGCG. The binding sites of many ZmMYC2 regulating genes are identified by IGV-sRNA. Conclusions All together, abundant target genes of ZmMYC2 are characterized with their binding sites, providing the basis to construct the regulatory network of ZmMYC2 and better understanding for JA signaling in maize.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Genome-wide identification of ZmMYC2 binding sites and target genes in maize

Liu,

Zhang,

Tang

et al. 2024

BMC Genomics

Self Cite

View full text Add to dashboard Cite

show abstract

“…Maize is a major staple crop planted in distinct agroecological zones. A large number of maize TFs have been verified to affect drought tolerance through different mechanisms, such as ZmNAC111, ZmERF21, and ZmHsf28 (Mao et al, 2015;Wang et al, 2022;Liu et al, 2023). In this study, we characterized a member of the AL family in maize, ZmAL14, which is a negative regulator in drought stress.…”

Section: Introductionmentioning

confidence: 99%

Phosphorylation of ZmAL14 by ZmSnRK2.2 regulates drought resistance through derepressing ZmROP8 expression

Wang,

Cheng,

Guo

et al. 2024

JIPB

View full text Add to dashboard Cite

Drought stress has negative effects on crop growth and production. Characterization of transcription factors that regulate the expression of drought‐responsive genes is critical for understanding the transcriptional regulatory networks in response to drought, which facilitates the improvement of crop drought tolerance. Here, we identified an Alfin‐like (AL) family gene ZmAL14 that negatively regulates drought resistance. Overexpression of ZmAL14 exhibits susceptibility to drought while mutation of ZmAL14 enhances drought resistance. An abscisic acid (ABA)‐activated protein kinase ZmSnRK2.2 interacts and phosphorylates ZmAL14 at T38 residue. Knockout of ZmSnRK2.2 gene decreases drought resistance of maize. A dehydration‐induced Rho‐like small guanosine triphosphatase gene ZmROP8 is directly targeted and repressed by ZmAL14. Phosphorylation of ZmAL14 by ZmSnRK2.2 prevents its binding to the ZmROP8 promoter, thereby releasing the repression of ZmROP8 transcription. Overexpression of ZmROP8 stimulates peroxidase activity and reduces hydrogen peroxide accumulation after drought treatment. Collectively, our study indicates that ZmAL14 is a negative regulator of drought resistance, which can be phosphorylated by ZmSnRK2.2 through the ABA signaling pathway, thus preventing its suppression on ZmROP8 transcription during drought stress response.

show abstract