Jasmonic acid and hydrogen sulfide modulate transcriptional and enzymatic changes of plasma membrane NADPH oxidases (NOXs) and decrease oxidative damage in Oryza sativa L. during thiocyanate exposure

Abstract

It is evident that the plasma membrane NADPH oxidases (NOXs) play an important role in the generation of superoxide radicals (O 2 −• ) in plants during defense responses. This study was to clarify activation of NOXs in oxidative damage in Oryza sativa during SCN − exposure, particularly in the roles of jasmonic acid (JA) and hydrogen sulfide (H 2 S) on transcriptional and enzymatic changes of NOXs. Results indicated that enzymatic activity of NOXs in both roots and shoots was significantly enhanced during SCN − exposure, whereas the application of JA and H 2 S donor (NaHS) significantly repressed NOXs activity in SCN-treated rice seedlings. Similarly, ROS analysis showed that SCN − exposure elevated the content of O 2 −• , hydrogen peroxide (H 2 O 2 ) and malondialdehyde (MDA) in rice tissues significantly, whereas decreases in O 2 −• and H 2 O 2 were detected in roots and shoots of SCN-treated rice seedlings due to application of JA and NaHS. PCR analysis revealed different expression patterns of 7 plasma membrane-localized NOX genes in rice roots and shoots against SCN − exposure, suggesting that various isogenes of NOXs might regulate and determine activity of NOXs in rice organs. In conclusion, SCN − exposure was able to trigger activation of NOXs effectively, and led to oxidative damage and lipid peroxidation; the effects of JA and NaHS on inactivation of NOXs was evident and tissue specific, which in turn modulated ROS accumulation in rice plants.

The Defensive Role of Endogenous H2S in Brassica rapa against Mercury-Selenium Combined Stress

“…Hg stress alone can induce NADPH oxidase-dependent ROS burst in plants as well [ 38 ]. It has been reported that H 2 S can protect both plant and mammalian cells from oxidative stress by repressing NADPH oxidase-dependent ROS generation [ 39 , 40 ]. Further studies are needed to understand whether H 2 S decreases ROS accumulation in plants against Hg + Se stress through the similar mechanisms mentioned above.…”

Gene network downstream plant stress response modulated by peroxisomal H2O2

“…Thus, in Arabidopsis plants, peroxisomal polyamine-dependent ROS production has been reported to promote the induction of NADPH oxidase activity which, in turn, induces an increase in oxygen consumption by an alternative mitochondrial oxidase pathway ( Andronis et al, 2014 ). Jasmonic acid produced in peroxisomes can also modulate transcriptional and enzymatic changes in plasma membrane NADPH oxidases in rice plants in response to thiocyanate ( Yu et al, 2021 ). The upregulation of CAT and GOX in Arabidopsis rboh mutants in response to Cd ( Gupta et al, 2017 ) points to a close relationship between peroxisomal ROS and NADPH oxidase-dependent ROS production which could play a key role in cellular redox homeostasis and signaling.…”

Signal Mediators in the Implementation of Jasmonic Acid’s Protective Effect on Plants under Abiotic Stresses

“…While there are many data indicating an increase in the activity of ROS-generating enzymes (especially NADPH oxidase), there are also data on the suppression of the activity of this enzyme by JA. In rice plants under cyanide-induced oxidative stress, exogenous JA reduces ROS content, NADPH oxidase activity, and expression of individual genes encoding the catalytic subunit of the enzyme [ 90 ]. The molecular mechanisms underlying the effects of JA on ROS-generating systems remain poorly understood.…”