Gene network downstream plant stress response modulated by peroxisomal H2O2

Abstract

Reactive oxygen species (ROS) act as secondary messengers that can be sensed by specific redox-sensitive proteins responsible for the activation of signal transduction culminating in altered gene expression. The subcellular site, in which modifications in the ROS/oxidation state occur, can also act as a specific cellular redox network signal. The chemical identity of ROS and their subcellular origin is actually a specific imprint on the transcriptome response. In recent years, a number of transcriptomic studies related to altered ROS metabolism in plant peroxisomes have been carried out. In this study, we conducted a meta-analysis of these transcriptomic findings to identify common transcriptional footprints for plant peroxisomal-dependent signaling at early and later time points. These footprints highlight the regulation of various metabolic pathways and gene families, which are also found in plant responses to several abiotic stresses. Major peroxisomal-dependent genes are associated with protein and endoplasmic reticulum (ER) protection at later stages of stress while, at earlier stages, these genes are related to hormone biosynthesis and signaling regulation. Furthermore, in silico analyses allowed us to assign human orthologs to some of the peroxisomal-dependent proteins, which are mainly associated with different cancer pathologies. Peroxisomal footprints provide a valuable resource for assessing and supporting key peroxisomal functions in cellular metabolism under control and stress conditions across species.

Diversification of gene expression across extremophytes and stress-sensitive species in the Brassicaceae

“…Glutathione S-transferases protect cells from oxidative damage by utilizing glutathione to scavenge reactive oxygen species (ROS) (S. Kumar & Trivedi, 2018). GSTU25 has been reported to be induced under oxidative, salt, and heat stresses (Sappl et al, 2009; Terrón-Camero et al, 2022). The convergent endodermal expression of GSTU25 in the two extremophytes might suggest a role of GSTU25 in ROS scavenging in endodermal cells, which may contribute to the salt tolerance in the two extremophytes.…”

Transcriptome sequencing of garlic reveals key genes related to the heat stress response

“…Under abiotic stress conditions, the accumulation of unfolded or misfolded proteins in the ER can lead to ER stres 25 . Li et al also found that the upregulation of ER protein processing in maize was most significant after high-temperature stress, which was consistent with our research results 26 .…”

Transcriptomic dynamics in the transition from ground to space are revealed by Virgin Galactic human-tended suborbital spaceflight

“…), primarily those represented in core DEGs of ROS Wheel Cluster III 15 . The ROS Cluster III genes are typical of a rapidresponse to high intensity light and are also represented in the rapid oxidative stress response of peroxisome-dependent genes 32 . The predominance of this category of ROS genes suggests that rapid changes in the gravity environment are perceived by plants as an acute stress requiring mitigation though the ROS signal transduction pathway.…”