Jasmonate‐induced biosynthesis of andrographolide in Andrographis paniculata

Abstract

Andrographolide is a prominent secondary metabolite found in Andrographis paniculata that exhibits enormous pharmacological effects. In spite of immense value, the normal biosynthesis of andrographolide results in low amount of the metabolite. To induce the biosynthesis of andrographolide, we attempted elicitor-induced activation of andrographolide biosynthesis in cell cultures of A. paniculata. This was carried out by using methyl jasmonate (MeJA) as an elicitor. Among the various concentrations of MeJA tested at different time periods, 5 µM MeJA yielded 5.25 times more andrographolide content after 24 h of treatment. The accumulation of andrographolide was correlated with the expression level of known regulatory genes (hmgs, hmgr, dxs, dxr, isph and ggps) of mevalonic acid (MVA) and 2-C-methyl-d-erythritol-4-phosphate (MEP) pathways. These results established the involvement of MeJA in andrographolide biosynthesis by inducing the transcription of its biosynthetic pathways genes. The coordination of isph, ggps and hmgs expression highly influenced the andrographolide biosynthesis.

A 1-deoxy-d-xylulose 5-phosphate reductoisomerase gene probably involved in the synthesis of terpenoids in Chrysanthemum indicum var. aromaticum

“…fil.) Nees, the expression of DXR was highest at 5.0 μmol L −1 (20.97 ± 1.39) followed by 10 μmol L −1 (11.00 ± 1.46) of MeJA at 48 h (Sharma et al 2015). Our results showed that the expression of CiDXR increased rapidly after MeJA treatment (Fig.…”

“…aromaticum. This study showed that MeJA can induce the biosynthesis of terpenoids and also showed that the transcript level of DXR was correlated with terpenoid content, which could help to elucidate the expression kinetics and regulation of the key governing enzymes of the terpenoid biosynthetic pathway (Sharma et al 2015).…”

“…This induced effect had also been shown in other species such as Camptotheca acuminate Decne. (Yao et al 2008), A. villosum (Wei et al 2013), T. wilfordii (Tong et al 2015), A. annua (Xiang et al 2015), A. sinensis (Liu et al 2015), and A. paniculata (Sharma et al 2015). These results indicated that the regulation of CiDXR was positively influenced by MeJA treatment.…”

Abiotic and Biotic Elicitors–Role in Secondary Metabolites Production through In Vitro Culture of Medicinal Plants

“…The MeJA enhanced the production of bacoside A, a valuable triterpenoid saponin having nootropic therapeutic activity in in vitro shoot cultures of Bacopa monnieri [96]. In Andrographis paniculata cell culture, the MeJA induced the production of andrographolide content [97]. In Glycyrrhiza glabra, methyl jasmonate induced the production of the saponin soyasaponin [98].…”

Impact of Abiotic Elicitors on In vitro Production of Plant Secondary Metabolites: A Review

“…The treatment of MeJA to V. vinifera cell cultures also promoted anthocyanin accumulation [71]. In the cell culture of Andrographis paniculata, the MeJA induced the optimum accumulation of andrographolide at 24 h compared with 48 and 72 h of treatments [72]. In the V. vinifera cell system, a rapid accumulation of trans-resveratrol was recorded with MeJA treatement, starting from 2 h and reaching its maximum value at 96 h [70].…”